V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian) (ERBB2) (pThr1221), (pThr1222) antibody

Details for Product No. ABIN649826
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Antigen
Synonyms CD340, HER-2, HER-2/neu, HER2, MLN 19, NEU, NGL, TKR1, Erbb-2, Neu, c-erbB2, c-neu, mKIAA3023
Epitope
pThr1221, pThr1222
(61), (49), (49), (45), (41), (38), (35), (28), (25), (22), (21), (16), (10), (9), (9), (9), (9), (9), (9), (9), (9), (9), (9), (8), (8), (8), (8), (8), (7), (7), (7), (7), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (5), (5), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(790), (332), (227), (91), (60), (48), (36), (24), (6), (4), (4), (2)
Host
Rabbit
(661), (191), (15), (11), (5), (1)
Clonality
Monoclonal
Conjugate
Un-conjugated
(48), (38), (31), (24), (22), (20), (10), (10), (10), (10), (10), (10), (10), (10)
Application
Western Blotting (WB)
(458), (302), (214), (200), (159), (151), (102), (100), (57), (46), (36), (10), (5), (3), (2), (1), (1), (1), (1), (1), (1)
Pubmed 4 references available
Quantity 100 μL
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Catalog No. ABIN649826
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Specificity A phosphor-specific peptide corresponding to residues surrounding tyrosine 1222 of human HER2 was used as an immunogen. This antibody detects HER2 phosphorylated at tyrosine 1222.
Alternative Name HER2
Background HER2 (ErbB2, epidermal growth factor receptor 2) is one of the four members of the ErbB receptor family of transmembrane receptor-like tyrosine kinases (1). The kinase activity of HER2 can be activated without ligand if it is overexpressed, and by association with other HER proteins (2). Overexpression of HER2 is detected in almost 40% of human breast cancers (3). HER2 is considered one of the major targets for the treatment of breast cancer and other carcinomas. Binding of c-Cbl ubiquitin ligase to Tyr1112 of HER2 leads to poly-ubiquitination of HER2 and enhances its degradation (4). Similar to other growth factors, HER2 exhibits intrinsic protein tyrosine kinase activity and undergoes autophosphorylation (Y1023, Y1248, Y1139, and Y1222). A phosphorylation at tyrosine 1139 has been identified to induce the binding of Src to HER2, leading to HER2 activation of Stat3 alpha (5).
Synonyms: ERBB2, HER2, NEU, NGL, Receptor tyrosine-protein kinase erbB-2, p185erbB2,C-erbB-2,NEU proto-oncogene,Tyrosine kinase-type cell surface receptor HER2,MLN 19
Molecular Weight 185 kDA
Gene ID 2064
UniProt P04626
Research Area Ubiquitin-related antibodies, Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Structure, Signaling, Chromatin
Application Notes The suggested dilution is: WB: = 1:5000
Comment

Background: HER2 (ErbB2, epidermal growth factor receptor 2) is one of the four members of the ErbB receptor family of transmembrane receptor-like tyrosine kinases (1). The kinase activity of HER2 can be activated without ligand if it is overexpressed, and by association with other HER proteins (2). Overexpression of HER2 is detected in almost 40% of human breast cancers (3). HER2 is considered one of the major targets for the treatment of breast cancer and other carcinomas. Binding of c-Cbl ubiquitin ligase to Tyr1112 of HER2 leads to poly-ubiquitination of HER2 and enhances its degradation (4). Similar to other growth factors, HER2 exhibits intrinsic protein tyrosine kinase activity and undergoes autophosphorylation (Y1023, Y1248, Y1139, and Y1222). A phosphorylation at tyrosine 1139 has been identified to induce the binding of Src to HER2, leading to HER2 activation of Stat3 alpha (5).

Restrictions For Research Use only
Format Liquid
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment HER2 Antibody Phospho (pT1221/T1222) can be stored at -20°C for up to 12 months from time of receipt.
Expiry Date 12 months
Background publications Segatto, Lonardo, Pierce et al.: "The role of autophosphorylation in modulation of erbB-2 transforming function." in: The New biologist, Vol. 2, Issue 2, pp. 187-95, 1991 (PubMed).

Muthuswamy, Gilman, Brugge: "Controlled dimerization of ErbB receptors provides evidence for differential signaling by homo- and heterodimers." in: Molecular and cellular biology, Vol. 19, Issue 10, pp. 6845-57, 2000 (PubMed).

Klapper, Waterman, Sela et al.: "Tumor-inhibitory antibodies to HER-2/ErbB-2 may act by recruiting c-Cbl and enhancing ubiquitination of HER-2." in: Cancer research, Vol. 60, Issue 13, pp. 3384-8, 2000 (PubMed).

Montgomery, Makary, Schiffman et al.: "Endogenous anti-HER2 antibodies block HER2 phosphorylation and signaling through extracellular signal-regulated kinase." in: Cancer research, Vol. 65, Issue 2, pp. 650-6, 2005 (PubMed).

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