V-Erb-A erythroblastic Leukemia Viral Oncogene Homolog 4 (Avian) (ERBB4) (C-Term) antibody

Antigen: V-Erb-A erythroblastic Leukemia Viral Oncogene Homolog 4 (Avian) (ERBB4)

Synonyms: erbb4, si:ch211-227n20.1, HER4, MGC138404, p180erbB4, Her4

Binding Site: C-Term

Clonality: Monoclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunoprecipitation (IP)

Certificates: ISO 9001:2008

Catalog no.: ABIN649830

Additional Information

Alternative name: HER4 (ErbB4)

Gene ID: 2066

UniProt: Q15303

Description: Other names: ERBB4, HER4, Receptor tyrosine-protein kinase erbB-4, p180erbB4,Tyrosine kinase-type cell surface receptor HER4

Characteristics: Rabbit Monoclonal Antibody

Specificity: A synthetic peptide corresponding to C-terminus (cytoplasmic domain) of human ErbB4 was used as immunogen. The antibody does not cross-react with other EGF receptor family members.

Molecular Weight: 185 kDA

Comments:

Background: HER4 / ErbB4 is a receptor tyrosine kinase that belongs to the human epidermal growth factor receptor (EGFR) family. erbB4/HER4 is most predominantly expressed in several breast carcinoma cell lines, and in normal skeletal muscle, heart, pituitary, brain, and cerebellum (1). erbB4/HER4 is activated by neuregulins (NRG), betacellulin (BTC), and heparin-binding EGF-like growth factor (2). Neuregulins regulate the expression of ligand- and voltage-gated channels in neurons and skeletal muscle by the activation of ErbB 1-4. The erbB4/HER4 receptor is unique among these receptors because its C-terminal tail binds to a protein motif known as the PDZ domain (3).

Application Details

Application Notes: The suggested dilution is: WB: ~~ 1:500 FC: ~~ 1:40 ICC: ~~ 1:50

Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage: HER4 (ErbB4) Antibody (C-term) can be stored at -20°C for up to 12 months from time of receipt.

Research Area: Ubiquitin-related antibodies, Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Structure, Signaling, Chromatin

Restrictions: For Research Use only

Publications

Product: Plowman, Culouscou, Whitney et al.: "Ligand-specific activation of HER4/p180erbB4, a fourth member of the epidermal growth factor receptor family." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 90, Issue 5, pp. 1746-50, 1993 (PubMed).

Elenius, Corfas, Paul et al.: "A novel juxtamembrane domain isoform of HER4/ErbB4. Isoform-specific tissue distribution and differential processing in response to phorbol ester." in: The Journal of biological chemistry, Vol. 272, Issue 42, pp. 26761-8, 1997 (PubMed).

Garcia, Vasudevan, Buonanno: "The neuregulin receptor ErbB-4 interacts with PDZ-containing proteins at neuronal synapses." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 97, Issue 7, pp. 3596-601, 2000 (PubMed).