V-Erb-A erythroblastic Leukemia Viral Oncogene Homolog 4 (Avian) (ERBB4) (pTyr1188) antibody

Antigen: V-Erb-A erythroblastic Leukemia Viral Oncogene Homolog 4 (Avian) (ERBB4)

Synonyms: erbb4, si:ch211-227n20.1, HER4, MGC138404, p180erbB4, Her4

Binding Site: pTyr1188

Clonality: Monoclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC)

Certificates: ISO 9001:2008

Catalog no.: ABIN649832

Additional Information

Alternative name: HER4 (ErbB4)

Gene ID: 2066

UniProt: Q15303

Description: Other names: ERBB4, HER4, Receptor tyrosine-protein kinase erbB-4, p180erbB4,Tyrosine kinase-type cell surface receptor HER4

Characteristics: Rabbit Monoclonal Antibody

Specificity: A phospho specific peptide corresponding to residues surrounding tyrosine 1188 of human Her4 was used as an immunogen. This antibody detects Her4 phosphorylated on tyrosine 1188.

Molecular Weight: 180 kDA

Comments:

Background: Her4 / ErbB4 is a receptor tyrosine kinase that belongs to the human epidermal growth factor receptor (EGFR) family. ErbB4/Her4 is most predominantly expressed in several breast carcinoma cell lines, and in normal skeletal muscle, heart, pituitary, brain, and cerebellum (1). ErbB4/Her4 is activated by neuregulins (NRG), betacellulin (BTC), and heparin-binding EGF-like growth factor (2). Neuregulins regulate the expression of ligand- and voltage-gated channels in neurons and skeletal muscle by the activation of ErbB 1-4. The erbB4/Her4 receptor is unique among these receptors because its C-terminal tail binds to a protein motif known as the PDZ domain (3).

Application Details

Application Notes: The suggested dilution is: WB: ~~ 1:1000~10000 ICC: ~~ 1:100

Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage: HER4 (ErbB4) Antibody Phospho (pY1188) can be stored at -20°C for up to 12 months from time of receipt.

Research Area: Ubiquitin-related antibodies, Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Structure, Signaling, Chromatin

Restrictions: For Research Use only

Publications

Product: Elenius, Corfas, Paul et al.: "A novel juxtamembrane domain isoform of HER4/ErbB4. Isoform-specific tissue distribution and differential processing in response to phorbol ester." in: The Journal of biological chemistry, Vol. 272, Issue 42, pp. 26761-8, 1997 (PubMed).

Garcia, Vasudevan, Buonanno: "The neuregulin receptor ErbB-4 interacts with PDZ-containing proteins at neuronal synapses." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 97, Issue 7, pp. 3596-601, 2000 (PubMed).