V-Erb-A erythroblastic Leukemia Viral Oncogene Homolog 4 (Avian) (ERBB4) (pTyr1056) antibody

Antigen: V-Erb-A erythroblastic Leukemia Viral Oncogene Homolog 4 (Avian) (ERBB4)

Synonyms: erbb4, si:ch211-227n20.1, HER4, MGC138404, p180erbB4, Her4

Binding Site: pTyr1056

Clonality: Monoclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN649834

Additional Information

Alternative name: Her4

Gene ID: 861

UniProt: Q15303

Characteristics: Rabbit Monoclonal Antibody

Specificity: A phospho-specific peptide corresponding to residues surrounding Tyrosine 1056 in human HER4/ErbB4 was used as an immunogen.

Molecular Weight: 180 kDA

Comments:

Background: HER4 / ErbB4 is a receptor tyrosine kinase that belongs to the human epidermal growth factor receptor (EGFR) family. ErbB4/HER4 is most predominantly expressed in several breast carcinoma cell lines, and in normal skeletal muscle, heart, pituitary, brain, and cerebellum (1). ErbB4/HER4 is activated by neuregulins (NRG), betacellulin (BTC), and heparin-binding EGF-like growth factor (2). Neuregulins regulate the expression of ligand- and voltage-gated channels in neurons and skeletal muscle by the activation of ErbB 1-4. The erbB4/HER4 receptor is unique among these receptors because its C-terminal tail binds to a protein motif known as the PDZ domain (3).

Application Details

Application Notes: The suggested dilution is: WB: ~~ 1:1000~10000

Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage: Her4 Antibody Phospho (pY1056) can be stored at -20°C for up to 12 months from time of receipt.

Research Area: Ubiquitin-related antibodies, Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Structure, Signaling, Chromatin

Restrictions: For Research Use only

Publications

Product: Plowman, Culouscou, Whitney et al.: "Ligand-specific activation of HER4/p180erbB4, a fourth member of the epidermal growth factor receptor family." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 90, Issue 5, pp. 1746-50, 1993 (PubMed).

Garcia, Vasudevan, Buonanno: "The neuregulin receptor ErbB-4 interacts with PDZ-containing proteins at neuronal synapses." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 97, Issue 7, pp. 3596-601, 2000 (PubMed).