HIAP-1 (BIR3) antibody

Antigen: HIAP-1 (BIR3)

Clonality: Monoclonal

Host: Rabbit

Reactivity: Human, Rat (Rattus)

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Certificates: ISO 9001:2008

Catalog no.: ABIN649836

Additional Information

Gene ID: 330

UniProt: Q13489

Description: Other names: BIRC3, API2, IAP1, MIHC, RNF49, Baculoviral IAP repeat-containing protein 3, RING finger protein 49

Characteristics: Rabbit Monoclonal Antibody

Specificity: A synthetic peptide corresponding to residues, before the breakpoint for translocation to form BIRC3-MALT1, of Human BIP3 was used as immunogen. The antibody does not cross-react with other IAP family members.

Molecular Weight: 72 kDA

Comments:

Background: cIAP-2, also known as HIAP-1, is a member of the inhibitor of apoptosis (IAP) family of proteins. These proteins contain a BIR (baculovirus IAP repeat) domain near the amino-terminus (1). cIAP-2 is localized to the cytoplasm. The BIR motifs region interacts with TNF receptor associated factors 1 and 2 (TRAF1 and TRAF2) to form a heteromeric complex, which is then recruited to the tumor necrosis factor receptor 2 (TNFR2). This complex is thought to play a role in inhibition of apoptosis (2,3). cIAP-2 inhibits cytochrome c-induced activation of caspase-9, thereby preventing initiation of the caspase cascade and apoptosis (4).

Application Details

Application Notes: The suggested dilution is: WB: ~~ 1:5000 IP: ~~ 1:50 ICC: ~~ 1:100 IHC: ~~ 1:50

Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage: HIAP-1 (BIR3) Antibody can be stored at -20°C for up to 12 months from time of receipt.

Research Area: Apoptosis/Necrosis, Signaling, Chromatin, Cell Structure

Restrictions: For Research Use only

Publications

Product: Rothe, Pan, Henzel et al.: "The TNFR2-TRAF signaling complex contains two novel proteins related to baculoviral inhibitor of apoptosis proteins." in: Cell, Vol. 83, Issue 7, pp. 1243-52, 1996 (PubMed).

Deveraux, Roy, Stennicke et al.: "IAPs block apoptotic events induced by caspase-8 and cytochrome c by direct inhibition of distinct caspases." in: The EMBO journal, Vol. 17, Issue 8, pp. 2215-23, 1998 (PubMed).