Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A) antibody

Antigen: Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A)

Synonyms: HIF1, MOP1, PASD8, bHLHe78, HIF-1alpha, HIF1-ALPHA, HIF1A, HIF-1a, HIF1a, hif-1a, hif1a

Clonality: Monoclonal

Host: Rabbit

Reactivity: Human, Rat (Rattus)

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Certificates: ISO 9001:2008

Catalog no.: ABIN649837

Additional Information

Alternative name: HIF-1 alpha

Gene ID: 3091

UniProt: Q16665

Description: Other names: HIF1A, MOP1, Hypoxia-inducible factor 1 alpha, ARNT-interacting protein,Member of PAS protein 1,Basic-helix-loop-helix-PAS protein MOP1

Characteristics: Rabbit Monoclonal Antibody

Specificity: A synthetic peptide corresponding to residues near the C-terminus of human HIF-1 alpha was used as an immunogen.

Molecular Weight: 120 kDA

Comments:

Background: The HIF1A gene encodes the HIF-1alpha subunit of hypoxia-inducible factor 1, a transcription factor that is essential for cardiovascular development and systemic O2 homeostasis (1). HIF-1 is found in mammalian cells cultured under reduced O2 tension and is necessary for transcriptional activation mediated by the erythropoietin gene enhancer in hypoxic cells. It has been shown that both HIF-1 subunits are basic-helix-loop-helix proteins containing a PAS domain, defined by its presence in the in the mammalian ARNT and AHR proteins. HIF-1 alpha is most closely related to Sim (2). HIF-1 binds to cis-acting hypoxia-response elements within the erythropoietin, vascular endothelial growth factor, and other genes to activate transcription in hypoxic cells. Results indicate that increased transcriptional activity mediated by HIF-1 in hypoxic cells results from both increased HIF-1alpha protein levels and increased activity of HIF-1alpha transactivation domains (3).

Application Details

Application Notes: The suggested dilution is: WB: ~~ 1:1000~2000 ICC: ~~ 1:100~250 IHC: ~~ 1:250~500

Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage: HIF-1 alpha Antibody can be stored at -20°C for up to 12 months from time of receipt.

Research Area: Ubiquitin-related antibodies, Translation Factors, Phospho-specific antibodies, Protein Modifications, Transcription Factors, Signaling, Cell Structure

Restrictions: For Research Use only

Publications

Product: Wang, Jiang, Rue et al.: "Hypoxia-inducible factor 1 is a basic-helix-loop-helix-PAS heterodimer regulated by cellular O2 tension." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 92, Issue 12, pp. 5510-4, 1995 (PubMed).

Jiang, Zheng, Leung et al.: "Transactivation and inhibitory domains of hypoxia-inducible factor 1alpha. Modulation of transcriptional activity by oxygen tension." in: The Journal of biological chemistry, Vol. 272, Issue 31, pp. 19253-60, 1997 (PubMed).

Iyer, Leung, Semenza: "The human hypoxia-inducible factor 1alpha gene: HIF1A structure and evolutionary conservation." in: Genomics, Vol. 52, Issue 2, pp. 159-65, 1998 (PubMed).