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Histon H2B Type 1B antibody

Antigen

Histon H2B Type 1B
Clonality Monoclonal
Host

Rabbit
Reactivity

Human
Application
Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
2 references available
Certificates ISO 9001:2008
Catalog no. ABIN649838
Quantity 100ul
Price 450.00 $   Plus shipping costs $45.00
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Additional Information
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Gene ID 440689
UniProt Q5QNW6
Description Other names: HIST2H2BF, Histone H2B type 2-F
Characteristics Rabbit Monoclonal Antibody
Specificity A synthetic peptide corresponding to residues on the N-terminus of human Histone H2B Type 1B was used as an immunogen.
Molecular Weight 14 kDA
Comments

Background: Changes in chromatin structure play a large role in the regulation of transcription in eukaryotes (1). The nucleosome is the primary building block of chromatin, and is made up of four core histone proteins (H2A, H2B, H3 and H4) (2). Acetylation of core histones regulates gene expression (2). Histone H2B is primarily acetylated at lysines 5, 12, 15, and 20 (3). Ubiquitylation of Histone H2B at lysine 123 by Rad6 has been implicated in transcriptional activation and lysine methylation on histone H3 (4).

Application Details
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Application Notes The suggested dilution is: WB: ~~ 1:1000~10000 ICC: ~~ 1:100~250 IHC: ~~ 1:100~250
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Storage Histon H2B Antibody Type 1B can be stored at -20°C for up to 12 months from time of receipt.
Research Area Ubiquitin-related antibodies, Signaling, Phospho-specific antibodies, Protein Modifications, Transcription Factors, Chromatin, Cell Structure
Restrictions For Research Use only

Publications
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Product Braunstein, Sobel, Allis et al.: "Efficient transcriptional silencing in Saccharomyces cerevisiae requires a heterochromatin histone acetylation pattern." in: Molecular and cellular biology, Vol. 16, Issue 8, pp. 4349-56, 1996 (PubMed).

Workman, Kingston: "Alteration of nucleosome structure as a mechanism of transcriptional regulation." in: Annual review of biochemistry, Vol. 67, pp. 545-79, 1998 (PubMed).