Histone H2B antibody

Antigen: Histone H2B

Synonyms: DmelCG17949, CG17949, H2B, FLJ35099, FLJ56780, FLJ56787, MGC131639, HISTONE 2B, HISTONE H2B, HTB9, HTB2, MRN17.11, MRN17_11

Clonality: Monoclonal

Host: Rabbit

Reactivity: Human, Rat (Rattus)

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Certificates: ISO 9001:2008

Catalog no.: ABIN649845

Additional Information

Gene ID: 440689

UniProt: Q5QNW6

Description: Other names: HIST2H2BF, Histone H2B type 2-F

Characteristics: Rabbit Polyclonal IgG Affinity Purified

Specificity: A synthetic peptide corresponding to residues of human histone H2B was used as an immunogen.

Molecular Weight: 14 kDA

Comments:

Background: Changes in chromatin structure play a key role in the regulation of transcription in eukaryotes (1). The nucleosome is the primary building block of chromatin, and is made up of four core histone proteins (H2A, H2B, H3 and H4) (2). Acetylation of core histones regulates gene expression (2). Histone H2B is acetylated in the N-terminal region, primary at lysines 5, 12, 15, and 20 (3). Ubiquitylation of histone H2B at lysine 123 by Rad6 has been implicated in transcriptional activation and lysine methylation on histone H3 (4). Histone H2B phosphorylation at Ser10 (for yeast) and Ser14 (for mammalian cells) has been linked to regulation of cell apoptosis and meiosis (5).

Application Details

Application Notes: The suggested dilution is: WB: ~~ 1:50000~100000 IP: ~~ 1:50 ICC: ~~ 1:50~100 IHC: ~~ 1:100~250

Purification: Purified

Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage: Histone H2B Antibody can be stored at -20°C for up to 12 months from time of receipt.

Research Area: Ubiquitin-related antibodies, Signaling, Phospho-specific antibodies, Protein Modifications, Transcription Factors, Chromatin, Cell Structure

Restrictions: For Research Use only

Publications

Product: Braunstein, Sobel, Allis et al.: "Efficient transcriptional silencing in Saccharomyces cerevisiae requires a heterochromatin histone acetylation pattern." in: Molecular and cellular biology, Vol. 16, Issue 8, pp. 4349-56, 1996 (PubMed).

Workman, Kingston: "Alteration of nucleosome structure as a mechanism of transcriptional regulation." in: Annual review of biochemistry, Vol. 67, pp. 545-79, 1998 (PubMed).