Search, Find and Buy Antibodies, ELISA Kits and Proteins.
Loading Loading
Order hotline:
phone +1 404 474 4654
fax +1 888 205 9894 (TF)
Details for Product No. ABIN649849

Histone H3 (HTR12) (acLys14) antibody

Request
Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Antigen
Synonyms H3/i, H3FI, h3, Hist1h3f, hist2h3c, CENH3, CENTROMERIC HISTONE H3, F6F3.17, F6F3_17
Epitope
»Alternatives acLys14
Reactivity
»Alternatives Human, Rat (Rattus)
Host
»Alternatives Rabbit
Clonality Monoclonal
Conjugate
»Alternatives Un-conjugated
Application
»Alternatives Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)
Pubmed 2 references available
Catalog no. ABIN649849
Quantity 100 µL
Price
507.14 $   Plus shipping costs $45.00
Shipping to
Availability Will be delivered in 2 to 3 Business Days
Specificity A synthetic acetyl-specific peptide corresponding to residues around Lysine 14 was used as an immunogen. This antibody recognizes Histone H3 acetylated on K14.
Alternative Name Histone H3
Background Changes in chromatin structure play a large role in the regulation of transcription in eukaryotes (1). The nucleosome is the primary building block of chromatin, and is made up of four core histone proteins (H2A, H2B, H3 and H4) (2). Acetylation of core histones regulates gene expression (2). Histone H3 is primarily acetylated at lysines 9, 14, 18, and 23 (3,4). Acetylation at lysine 9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms (3,4). . H3t (H.34) differs from the consensus mammalian H3 structure by four amino acid residues and it is mainly expressed in testicular cells. H3t encoding gene maps to chromosome 1 (1q42), unlike the majority of the human histone genes that form cluster on chromosome 6 and outside of replication-dependent H3 genes (5).
Synonyms: HIST1H3J, H3FJ, Histone H3.1, H3/a,H3/b,H3/c,H3/d,H3/f,H3/h,H3/i,H3/j,H3/k,H3/l
Molecular Weight 17 kDA
Gene ID 8356, 8352, 8350
UniProt P68431
Research Area Ubiquitin-related antibodies, Signaling, Phospho-specific antibodies, Protein Modifications, Transcription Factors, Chromatin, Cell Structure
Application Notes The suggested dilution is: WB: ~~ 1:500 IP: ~~ 1:30 ICC: ~~ 1:250~500 IHC: ~~ 1:100~250
Comment

Background: Changes in chromatin structure play a large role in the regulation of transcription in eukaryotes (1). The nucleosome is the primary building block of chromatin, and is made up of four core histone proteins (H2A, H2B, H3 and H4) (2). Acetylation of core histones regulates gene expression (2). Histone H3 is primarily acetylated at lysines 9, 14, 18, and 23 (3,4). Acetylation at lysine 9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms (3,4). . H3t (H.34) differs from the consensus mammalian H3 structure by four amino acid residues and it is mainly expressed in testicular cells. H3t encoding gene maps to chromosome 1 (1q42), unlike the majority of the human histone genes that form cluster on chromosome 6 and outside of replication-dependent H3 genes (5).

Restrictions For Research Use only
Format Liquid
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Preservative Sodium azide
Storage -20 °C
Storage Comment Histone H3 Antibody Acetyl (K14) can be stored at -20°C for up to 12 months from time of receipt.
Expiry Date 12 months
Background publications Braunstein, Sobel, Allis et al.: "Efficient transcriptional silencing in Saccharomyces cerevisiae requires a heterochromatin histone acetylation pattern." in: Molecular and cellular biology, Vol. 16, Issue 8, pp. 4349-56, 1996 (PubMed).

Workman, Kingston: "Alteration of nucleosome structure as a mechanism of transcriptional regulation." in: Annual review of biochemistry, Vol. 67, pp. 545-79, 1998 (PubMed).

Alternatives for antigen "Histone H3 (HTR12)", type "Antibodies"
Hosts (455), (34), (8), (2)
Reactivities (462), (303), (299), (174), (156), (12), (8), (8), (5), (4), (3), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1)
Applications (316), (188), (159), (128), (95), (83), (50), (45), (38), (24), (13), (12), (1)
Conjugates (18), (17), (17), (16), (16), (16), (16), (16), (16), (16), (16), (2), (1)
Epitopes (40), (35), (30), (29), (25), (23), (21), (18), (17), (16), (16), (14), (14), (14), (11), (10), (9), (7), (7), (6), (5), (5), (5), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Beacon