Histone Cluster 3, H3 (HIST3H3) (pSer10) antibody

Details for Product No. ABIN649850
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Antigen
Synonyms H3.4, H3/g, H3FT, H3t, CG33848, Dmel\CG33848, H3.2-221, Hist1, H3/i, H3FI, h3, Hist1h3f, hist2h3c, T6J4.12, T6J4_12
Epitope
pSer10
(56), (48), (40), (31), (31), (25), (25), (24), (22), (20), (19), (19), (18), (17), (17), (16), (15), (11), (10), (10), (10), (10), (9), (9), (8), (8), (8), (7), (7), (7), (7), (6), (6), (5), (5), (5), (5), (5), (5), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human, Rat (Rattus)
(707), (426), (420), (182), (169), (12), (7), (5), (4), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Host
Rabbit
(644), (81), (8), (2)
Clonality
Monoclonal
Conjugate
Un-conjugated
(29), (21), (20), (18), (17), (17), (17), (17), (17), (17), (17), (11), (11), (11), (2)
Application
Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
(529), (242), (194), (190), (169), (94), (65), (59), (50), (38), (28), (8), (1), (1)
Pubmed 2 references available
Quantity 100 µL
Options
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Catalog No. ABIN649850
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Specificity A synthetic phospho-peptide corresponding to residues surrounding Ser10 of Human Histone H3 was used as immunogen. The antibody only detects Histone H3 phosphorylated on Serine 10. Predicted to cross-react with most species, based on sequence homology.
Alternative Name Histone H3
Background Changes in chromatin structure play a large role in the regulation of transcription in eukaryotes (1). The nucleosome is the primary building block of chromatin, and is made up of four core histone proteins (H2A, H2B, H3 and H4) (2). Acetylation of core histones regulates gene expression (2). Histone H3 is primarily acetylated at lysines 9, 14, 18, and 23 (3,4). Acetylation at lysine 9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms (3,4). Phosphorylation at Ser10 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis (5).
Synonyms: HIST3H3, H3FT, Histone H3.1t, H3/g
Molecular Weight 17 kDA
Gene ID 8290
UniProt Q16695
Research Area Ubiquitin-related antibodies, Signaling, Phospho-specific antibodies, Protein Modifications, Transcription Factors, Chromatin, Cell Structure
Application Notes The suggested dilution is: WB: = 1:200000-1000000 , IHC: = 1:50-100
Comment

Background: Changes in chromatin structure play a large role in the regulation of transcription in eukaryotes (1). The nucleosome is the primary building block of chromatin, and is made up of four core histone proteins (H2A, H2B, H3 and H4) (2). Acetylation of core histones regulates gene expression (2). Histone H3 is primarily acetylated at lysines 9, 14, 18, and 23 (3,4). Acetylation at lysine 9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms (3,4). Phosphorylation at Ser10 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis (5).

Restrictions For Research Use only
Format Liquid
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Histone H3 Antibody Phospho (pS10) can be stored at -20°C for up to 12 months from time of receipt.
Expiry Date 12 months
Background publications Braunstein, Sobel, Allis et al.: "Efficient transcriptional silencing in Saccharomyces cerevisiae requires a heterochromatin histone acetylation pattern." in: Molecular and cellular biology, Vol. 16, Issue 8, pp. 4349-56, 1996 (PubMed).

Workman, Kingston: "Alteration of nucleosome structure as a mechanism of transcriptional regulation." in: Annual review of biochemistry, Vol. 67, pp. 545-79, 1998 (PubMed).

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