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Histone H3 (pSer10) antibody
Human, Rat (Rattus)
Alternatives Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)
|2 references available|
|Price||507.14 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
|Description||Other names: HIST3H3, H3FT, Histone H3.1t, H3/g|
|Characteristics||Rabbit Monoclonal Antibody|
|Specificity||A synthetic phospho-peptide corresponding to residues surrounding Ser10 of Human Histone H3 was used as immunogen. The antibody only detects Histone H3 phosphorylated on Serine 10. Predicted to cross-react with most species, based on sequence homology.|
|Molecular Weight||17 kDA|
Background: Changes in chromatin structure play a large role in the regulation of transcription in eukaryotes (1). The nucleosome is the primary building block of chromatin, and is made up of four core histone proteins (H2A, H2B, H3 and H4) (2). Acetylation of core histones regulates gene expression (2). Histone H3 is primarily acetylated at lysines 9, 14, 18, and 23 (3,4). Acetylation at lysine 9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms (3,4). Phosphorylation at Ser10 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis (5).
|Synonyms||H3t, H3.4, H3/g, H3FT, MGC126886, MGC126888, Hist1, H3.2-221, DmelCG31613, CG31613, h3.2, h3/m, h3f2, h3fm, hist1h3g, H3F3, H3.3A, H3F3B, MGC87782, MGC87783, H3.3B, H3F3A, h3f3a, MGC52829, MGC86731, MGC165454, zgc:56193, zgc:86731, wu:fb58e10, FLJ35099, FLJ56780, FLJ56787, MGC131639, MGC89877, HIST1H3A|
|Application Notes||The suggested dilution is: WB: ~~ 1:200000~1000000 IP: ~~ 1:40 ICC: ~~ 1:250 IHC: ~~ 1:50~100|
|Buffer||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Histone H3 Antibody Phospho (pS10) can be stored at -20°C for up to 12 months from time of receipt.|
|Research Area||Ubiquitin-related antibodies, Signaling, Phospho-specific antibodies, Protein Modifications, Transcription Factors, Chromatin, Cell Structure|
|Restrictions||For Research Use only|
Braunstein, Sobel, Allis et al.: "Efficient transcriptional silencing in Saccharomyces cerevisiae requires a heterochromatin histone acetylation pattern." in: Molecular and cellular biology, Vol. 16, Issue 8, pp. 4349-56, 1996 (PubMed).
Workman, Kingston: "Alteration of nucleosome structure as a mechanism of transcriptional regulation." in: Annual review of biochemistry, Vol. 67, pp. 545-79, 1998 (PubMed).