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Details for Product No. ABIN649852

Histone H3 (HTR12) (pThr3) antibody

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Antigen
Synonyms H3/i, H3FI, h3, Hist1h3f, hist2h3c, CENH3, CENTROMERIC HISTONE H3, F6F3.17, F6F3_17
Epitope
»Alternatives pThr3
Reactivity
»Alternatives Human
Host
»Alternatives Rabbit
Clonality Monoclonal
Conjugate
»Alternatives Un-conjugated
Application
»Alternatives Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
Pubmed 2 references available
Catalog no. ABIN649852
Quantity 100 µL
Price
507.14 $   Plus shipping costs $45.00
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Specificity A phospho specific peptide corresponding to residues surrounding Threonine 3 of human Histone H3 was used as an immunogen. This antibody detects Histone H3 phosphorylated at Theronine 3.
Alternative Name Histone H3
Background Changes in chromatin structure play a large role in the regulation of transcription in eukaryotes (1). The nucleosome is the primary building block of chromatin, and is made up of four core histone proteins (H2A, H2B, H3 and H4) (2). Acetylation of core histones regulates gene expression (2). Histone H3 is primarily acetylated at lysines 9, 14, 18, and 23 (3,4). Acetylation at lysine 9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms (3,4). Immunostaining with phospho-specific antibodies in mammalian cells reveals mitotic phosphorylation of H3 Thr 3 in prophase and its dephosphorylation during anaphase. Furthermore we find that haspin, a member of a distinctive group of protein kinases present in diverse eukaryotes, phosphorylates H3 at Thr 3 in vitro. (5).
Synonyms: HIST1H3J, H3FJ, Histone H3.1, H3/a,H3/b,H3/c,H3/d,H3/f,H3/h,H3/i,H3/j,H3/k,H3/l
Molecular Weight 17 kDA
Gene ID 8968, 8357, 8356
UniProt P68431
Research Area Ubiquitin-related antibodies, Signaling, Phospho-specific antibodies, Protein Modifications, Transcription Factors, Chromatin, Cell Structure
Application Notes The suggested dilution is: WB: ~~ 1:10000~50000 ICC: ~~ 1:100~250 IHC: ~~ 1:100~250
Comment

Background: Changes in chromatin structure play a large role in the regulation of transcription in eukaryotes (1). The nucleosome is the primary building block of chromatin, and is made up of four core histone proteins (H2A, H2B, H3 and H4) (2). Acetylation of core histones regulates gene expression (2). Histone H3 is primarily acetylated at lysines 9, 14, 18, and 23 (3,4). Acetylation at lysine 9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms (3,4). Immunostaining with phospho-specific antibodies in mammalian cells reveals mitotic phosphorylation of H3 Thr 3 in prophase and its dephosphorylation during anaphase. Furthermore we find that haspin, a member of a distinctive group of protein kinases present in diverse eukaryotes, phosphorylates H3 at Thr 3 in vitro. (5).

Restrictions For Research Use only
Format Liquid
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Preservative Sodium azide
Storage -20 °C
Storage Comment Histone H3 Antibody Phospho (pT3) can be stored at -20°C for up to 12 months from time of receipt.
Expiry Date 12 months
Background publications Braunstein, Sobel, Allis et al.: "Efficient transcriptional silencing in Saccharomyces cerevisiae requires a heterochromatin histone acetylation pattern." in: Molecular and cellular biology, Vol. 16, Issue 8, pp. 4349-56, 1996 (PubMed).

Workman, Kingston: "Alteration of nucleosome structure as a mechanism of transcriptional regulation." in: Annual review of biochemistry, Vol. 67, pp. 545-79, 1998 (PubMed).

Alternatives for antigen "Histone H3 (HTR12)", type "Antibodies"
Hosts (455), (34), (8), (2)
Reactivities (462), (303), (300), (174), (156), (12), (8), (8), (5), (4), (3), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1)
Applications (316), (188), (159), (128), (95), (83), (51), (45), (38), (24), (13), (12), (1)
Conjugates (18), (17), (17), (16), (16), (16), (16), (16), (16), (16), (16), (2), (1)
Epitopes (40), (35), (29), (29), (25), (23), (21), (18), (17), (16), (16), (14), (14), (14), (11), (10), (9), (7), (7), (6), (6), (5), (5), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
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