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Histone H3 (pThr3) antibody

Antigen

Histone H3
Synonyms
H3t, H3.4, H3/g, H3FT, MGC126886, MGC126888, Hist1, H3.2-221, DmelCG31613, CG31613, h3.2, h3/m, h3f2, h3fm, hist1h3g, H3F3, H3.3A, H3F3B, MGC87782, MGC87783, H3.3B, H3F3A, h3f3a, MGC52829, MGC86731, M ... show more
Binding Site
Alternatives

pThr3
Clonality Monoclonal
Host
Alternatives

Rabbit
Reactivity
Alternatives

Human
Conjugate
Alternatives Un-conjugated
Application
Alternatives Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
2 references available
Certificates ISO 9001:2008
Catalog no. ABIN649852
Quantity 100ul
Price 507.14 $   Plus shipping costs $45.00
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Additional Information
Application Details Publications Alternatives back to top

Gene ID 8968, 8357, 8356, 8351, 8353, 8350, 8355, 8354, 83
UniProt P68431
Description Other names: HIST1H3J, H3FJ, Histone H3.1, H3/a,H3/b,H3/c,H3/d,H3/f,H3/h,H3/i,H3/j,H3/k,H3/l
Characteristics Rabbit Monoclonal Antibody
Specificity A phospho specific peptide corresponding to residues surrounding Threonine 3 of human Histone H3 was used as an immunogen. This antibody detects Histone H3 phosphorylated at Theronine 3.
Molecular Weight 17 kDA
Comments

Background: Changes in chromatin structure play a large role in the regulation of transcription in eukaryotes (1). The nucleosome is the primary building block of chromatin, and is made up of four core histone proteins (H2A, H2B, H3 and H4) (2). Acetylation of core histones regulates gene expression (2). Histone H3 is primarily acetylated at lysines 9, 14, 18, and 23 (3,4). Acetylation at lysine 9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms (3,4). Immunostaining with phospho-specific antibodies in mammalian cells reveals mitotic phosphorylation of H3 Thr 3 in prophase and its dephosphorylation during anaphase. Furthermore we find that haspin, a member of a distinctive group of protein kinases present in diverse eukaryotes, phosphorylates H3 at Thr 3 in vitro. (5).
Synonyms H3t, H3.4, H3/g, H3FT, MGC126886, MGC126888, Hist1, H3.2-221, DmelCG31613, CG31613, h3.2, h3/m, h3f2, h3fm, hist1h3g, H3F3, H3.3A, H3F3B, MGC87782, MGC87783, H3.3B, H3F3A, h3f3a, MGC52829, MGC86731, MGC165454, zgc:56193, zgc:86731, wu:fb58e10, FLJ35099, FLJ56780, FLJ56787, MGC131639, MGC89877, HIST1H3A

Application Details
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Application Notes The suggested dilution is: WB: ~~ 1:10000~50000 ICC: ~~ 1:100~250 IHC: ~~ 1:100~250
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Storage Histone H3 Antibody Phospho (pT3) can be stored at -20°C for up to 12 months from time of receipt.
Research Area Ubiquitin-related antibodies, Signaling, Phospho-specific antibodies, Protein Modifications, Transcription Factors, Chromatin, Cell Structure
Restrictions For Research Use only

Publications
Application Details Alternatives back to top

Product Braunstein, Sobel, Allis et al.: "Efficient transcriptional silencing in Saccharomyces cerevisiae requires a heterochromatin histone acetylation pattern." in: Molecular and cellular biology, Vol. 16, Issue 8, pp. 4349-56, 1996 (PubMed).

Workman, Kingston: "Alteration of nucleosome structure as a mechanism of transcriptional regulation." in: Annual review of biochemistry, Vol. 67, pp. 545-79, 1998 (PubMed).

Alternatives
Application Details Publications back to top

Alternatives for antigen "Histone H3", type "Antibodies"
Hosts Rabbit (188), Mouse (26), Sheep (6)
Reactivities Human (196), Rat (Rattus) (91), Mouse (Murine) (82), Cow (Bovine) (42), Fruit Fly (Drosophila melanogaster) (38), Pig (Porcine) (38), All Species (1), Arabidopsis thaliana (1), Mammalian (1), Rabbit (1), Rice (1), Tetrahymena (1)
Applications Western Blotting (WB) (149), Immunofluorescence (IF) (88), ELISA (86), Immunohistochemistry (IHC) (45), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (27), Immunocytochemistry (ICC) (22), Immunoprecipitation (IP) (19), Flow Cytometry (FACS) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Dot Blot (Dot) (1), Functional Studies (Func) (1), Immunoelectron Microscopy (IEM) (1)
Conjugates Alexa Fluor 488 (8), Alexa Fluor 350 (7), Alexa Fluor 555 (7), Alexa Fluor 647 (7), PE,Cy5.5 (7), Agarose Beads (2), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy7 (1)
Epitopes pSer10 (25), N-Term (17), pSer28 (17), pThr11 (13), pThr3 (9), C-Term (7), 2meLys9 (6), 3meLys4 (4), Lys27 (4), acLys14 (4), acLys9 (4), meLys9 (4), Lys4 (3), meLys4 (3), 2meLys27 (2), 2meLys4 (2), 3meLys27 (2), Lys36 (2), Lys9 (2), Mono Methyl Lysine 4 (2), Mono/bis-Citrulline 2-8-17 (2), Tri Methyl Lysine 4 (2), acLys18 (2), acLys23 (2), acLys27 (2), acLys56 (2), acetylated (2), ciArg2, ciArg8, ciArg17 (2), 2meArg2 (1), 2meArg8 (1), 2meLys4, 2meLys9 (1), 3meLys36 (1), 3meLys9 (1), AA 66-82 (1), AA 7-20 (1), Arg2 (1), Lys14,Lys9 (1), Lys79 (1), N Term, asymmetric dimethyl Arg2 (R2ADMA) (1), Ser10 (1), meLys9, 2meLys9, 3meLys9 (1), pSer10,pSer28 (1), pSer140 (1), pSer24,pSer20 (1)