Histone H3.3 (pSer31) antibody

Antigen: Histone H3.3

Synonyms: H3.3A, H3f3b, MGC102150, H3.3B, H3f3a, 9430068D06Rik, H3.3, His3.3, HisH3-3Q, PH3, anon-EST:fe1B6, DmelCG8989, CG8989, DmelCG5825, CG5825, MGC105794, MGC75998, h3f3a, h3f3c, h3f3d, wu:fa25h06, wu:fa96g06, wu:fb07a08, wu:fb36f01, zgc:110292, zgc:174300, DKFZp459A2130

Binding Site: pSer31

Clonality: Monoclonal

Host: Rabbit

Reactivity: Human, Rat (Rattus)

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Certificates: ISO 9001:2008

Catalog no.: ABIN649853

Additional Information

Gene ID: 3021, 3020

UniProt: P84243

Characteristics: Rabbit Monoclonal

Specificity: A synthetic peptide corresponding to residues surrounding Serine 31 of human Histone H3.3 was used as an immunogen.

Molecular Weight: 17 kDA

Comments:

Background: Changes in chromatin structure play a large role in the regulation of transcription in eukaryotes (1). The nucleosome is the primary building block of chromatin, and is made up of four core histone proteins (H2A, H2B, H3 and H4) (2). Various post-translational modifications, such as acetylation and methylation, of core histones serve as regulators of gene expression (2). Histone H3.3 is a variant histone which replaces conventional H3 in nucleosomes in active genes. It is predominant from of histone H3 in non-dividing cells and it is incorporated into chromatin independently of DNA synthesis. H3.3 is phosphorylated at serine 28 by MLTK isoform 1, RPS6KA5, and AURKB during mitosis. Also, phosphorylation at serine 31 on H3.3 is specific to regions bordering centromeres in metaphase chromosomes.

Synonyms: H3.3A, H3f3b, MGC102150, H3.3B, H3f3a, 9430068D06Rik, H3.3, His3.3, HisH3-3Q, PH3, anon-EST:fe1B6, DmelCG8989, CG8989, DmelCG5825, CG5825, MGC105794, MGC75998, h3f3a, h3f3c, h3f3d, wu:fa25h06, wu:fa96g06, wu:fb07a08, wu:fb36f01, zgc:110292, zgc:174300, DKFZp459A2130

Application Details

Application Notes: The suggested dilution is: WB: ~~ 1:1000~5000 ICC: ~~ 1:100~250 IHC: ~~ 1:100~250

Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage: Histone H3.3 Antibody Phospho (pS31) can be stored at -20°C for up to 12 months from time of receipt.

Research Area: Ubiquitin-related antibodies, Signaling, Phospho-specific antibodies, Protein Modifications, Transcription Factors, Chromatin, Cell Structure

Restrictions: For Research Use only

Publications

Product: Braunstein, Sobel, Allis et al.: "Efficient transcriptional silencing in Saccharomyces cerevisiae requires a heterochromatin histone acetylation pattern." in: Molecular and cellular biology, Vol. 16, Issue 8, pp. 4349-56, 1996 (PubMed).

Albig, Ebentheuer, Klobeck et al.: "A solitary human H3 histone gene on chromosome 1." in: Human genetics, Vol. 97, Issue 4, pp. 486-91, 1996 (PubMed).

Workman, Kingston: "Alteration of nucleosome structure as a mechanism of transcriptional regulation." in: Annual review of biochemistry, Vol. 67, pp. 545-79, 1998 (PubMed).

Xiao, Kao, Krogan et al.: "Histone H2B ubiquitylation is associated with elongating RNA polymerase II." in: Molecular and cellular biology, Vol. 25, Issue 2, pp. 637-51, 2005 (PubMed).