Histone H2A (acLys5) antibody

Antigen: Histone H2A

Synonyms: histone H2A 11, HTA11, histone H2A 4, HTA4, T6G15.120, T6G15_120, histone H2A, HTA2, M4I22.40, M4I22_40, ATHTA1, histone H2A 1, HTA1, MRB17.14, MRB17_14, RAT5, RESISTANT TO AGROBACTERIUM TRANSFORMATION 5

Binding Site: acLys5

Clonality: Monoclonal

Host: Rabbit

Reactivity: Human, Rat (Rattus)

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Certificates: ISO 9001:2008

Catalog no.: ABIN649854

Additional Information

Alternative name: Histone-H2A

Gene ID: 221613

UniProt: Q96QV6

Description: Other names: HIST1H2AA, H2AFR, Histone H2A type 1-A, H2A/r

Characteristics: Rabbit Monoclonal Antibody

Specificity: A synthetic peptide corresponding to residues near the N-terminus of human Histone H2A was used as an immunogen.

Molecular Weight: 14 kDA

Comments:

Background: Changes in chromatin structure play a large role in the regulation of transcription in eukaryotes (1). The nucleosome is the primary building block of chromatin, and is made up of four core histone proteins (H2A, H2B, H3 and H4) (2). It has been shown that DNA damage induces monoubiquitylation of histone H2A in the vicinity of DNA lesions. Ultraviolet -induced monoubiquitylation of H2A is dependent on functional nucleotide excision repair. It occurs after incision of the damaged strand and forms part of the cellular response to UV damage and suggests a role of this modification in DNA repair-induced chromatin remodeling (3).

Synonyms: histone H2A 11, HTA11, histone H2A 4, HTA4, T6G15.120, T6G15_120, histone H2A, HTA2, M4I22.40, M4I22_40, ATHTA1, histone H2A 1, HTA1, MRB17.14, MRB17_14, RAT5, RESISTANT TO AGROBACTERIUM TRANSFORMATION 5

Application Details

Application Notes: The suggested dilution is: WB: ~~ 1:1000 ICC: ~~ 1:500~1000 IHC: ~~ 1:250~500

Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage: Histone-H2A Antibody Acetyl (K5) can be stored at -20°C for up to 12 months from time of receipt.

Research Area: Ubiquitin-related antibodies, Signaling, Phospho-specific antibodies, Protein Modifications, Transcription Factors, Chromatin, Cell Structure

Restrictions: For Research Use only

Publications

Product: Braunstein, Sobel, Allis et al.: "Efficient transcriptional silencing in Saccharomyces cerevisiae requires a heterochromatin histone acetylation pattern." in: Molecular and cellular biology, Vol. 16, Issue 8, pp. 4349-56, 1996 (PubMed).

Workman, Kingston: "Alteration of nucleosome structure as a mechanism of transcriptional regulation." in: Annual review of biochemistry, Vol. 67, pp. 545-79, 1998 (PubMed).

Bergink, Salomons, Hoogstraten et al.: "DNA damage triggers nucleotide excision repair-dependent monoubiquitylation of histone H2A." in: Genes & development, Vol. 20, Issue 10, pp. 1343-52, 2006 (PubMed).