Histone H2A (acLys5) antibody

Details for Product No. ABIN649854
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Antigen
Synonyms
histone H2A 11, T6G15.120, T6G15_120, histone H2A 4, M4I22.40, M4I22_40, histone H2A 2, DDBDRAFT_0205974, DDBDRAFT_0216271, DDB_0205974, DDB_0216271, H2A-III, HIST1H2AA, RGD1564767, H2a-221, 5499, CG5 ... show more
histone H2A 11, T6G15.120, T6G15_120, histone H2A 4, M4I22.40, M4I22_40, histone H2A 2, DDBDRAFT_0205974, DDBDRAFT_0216271, DDB_0205974, DDB_0216271, H2A-III, HIST1H2AA, RGD1564767, H2a-221, 5499, CG5499, Dmel\\CG5499, H2A, H2A.F/Z, H2A.X, H2A.Z, H2AV, H2AV_DROM, H2AX, H2AZ, H2Av, H2AvD, H2a.V, H2av, H2avD, HIS, HIS2AV, HIS2AVD, His, His2, His2AV, His2AvD, HisH2Av, Hist, Hist2av, gamma-H2Av, gamma-HIS2AV, gamma-His2Av, gammaH2Av, h2AvD, his, l(3)05146, l(3)810, l(3)97Dd, l(3)L1602 show less
Epitope
acLys5
(22), (13), (11), (5), (4), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human, Rat (Rattus)
(99), (69), (66), (12), (12), (12), (2), (2), (2), (1), (1), (1)
Host
Rabbit
(100), (2)
Clonality
Monoclonal
Conjugate
Un-conjugated
(2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application
Western Blotting (WB), Immunohistochemistry (IHC)
(47), (41), (37), (22), (20), (13), (6), (4)
Pubmed 3 references available
Quantity 100 µL
Options
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Catalog No. ABIN649854
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Specificity A synthetic peptide corresponding to residues near the N-terminus of human Histone H2A was used as an immunogen.
Alternative Name Histone-H2A
Background Changes in chromatin structure play a large role in the regulation of transcription in eukaryotes (1). The nucleosome is the primary building block of chromatin, and is made up of four core histone proteins (H2A, H2B, H3 and H4) (2). It has been shown that DNA damage induces monoubiquitylation of histone H2A in the vicinity of DNA lesions. Ultraviolet -induced monoubiquitylation of H2A is dependent on functional nucleotide excision repair. It occurs after incision of the damaged strand and forms part of the cellular response to UV damage and suggests a role of this modification in DNA repair-induced chromatin remodeling (3).
Synonyms: HIST1H2AA, H2AFR, Histone H2A type 1-A, H2A/r
Molecular Weight 14 kDA
Gene ID 221613
UniProt Q96QV6
Research Area Ubiquitin-related antibodies, Signaling, Phospho-specific antibodies, Protein Modifications, Transcription Factors, Chromatin, Cell Structure
Application Notes The suggested dilution is: WB: = 1:1000 , IHC: = 1:250-500
Comment

Background: Changes in chromatin structure play a large role in the regulation of transcription in eukaryotes (1). The nucleosome is the primary building block of chromatin, and is made up of four core histone proteins (H2A, H2B, H3 and H4) (2). It has been shown that DNA damage induces monoubiquitylation of histone H2A in the vicinity of DNA lesions. Ultraviolet -induced monoubiquitylation of H2A is dependent on functional nucleotide excision repair. It occurs after incision of the damaged strand and forms part of the cellular response to UV damage and suggests a role of this modification in DNA repair-induced chromatin remodeling (3).

Restrictions For Research Use only
Format Liquid
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Histone-H2A Antibody Acetyl (K5) can be stored at -20°C for up to 12 months from time of receipt.
Expiry Date 12 months
Background publications Braunstein, Sobel, Allis et al.: "Efficient transcriptional silencing in Saccharomyces cerevisiae requires a heterochromatin histone acetylation pattern." in: Molecular and cellular biology, Vol. 16, Issue 8, pp. 4349-56, 1996 (PubMed).

Workman, Kingston: "Alteration of nucleosome structure as a mechanism of transcriptional regulation." in: Annual review of biochemistry, Vol. 67, pp. 545-79, 1998 (PubMed).

Bergink, Salomons, Hoogstraten et al.: "DNA damage triggers nucleotide excision repair-dependent monoubiquitylation of histone H2A." in: Genes & development, Vol. 20, Issue 10, pp. 1343-52, 2006 (PubMed).

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