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Histone H2B (acLys15) antibody

Antigen

Histone H2B
Synonyms DmelCG17949, CG17949, H2B, FLJ35099, FLJ56780, FLJ56787, MGC131639, HISTONE 2B, HISTONE H2B, HTB9, HTB2, MRN17.11, MRN17_11
Binding Site
Alternatives

acLys15
Clonality Monoclonal
Host
Alternatives

Rabbit
Reactivity
Alternatives

Human, Rat (Rattus)
Conjugate
Alternatives Un-conjugated
Application
Alternatives Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
2 references available
Certificates ISO 9001:2008
Catalog no. ABIN649856
Quantity 100ul
Price 507.14 $   Plus shipping costs $45.00
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Additional Information
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Alternative name Histone-H2B
Gene ID 440689
UniProt Q5QnW6
Description Other names: HIST2H2BF, Histone H2B type 2-F
Characteristics Rabbit Monoclonal Antibody
Specificity A synthetic acetylated peptide corresponding to residues surrounding Lysine 15 of Histone H2B was used as an immunogen. The antibody only detects Histone H2B acetylated on Lysine 15.
Molecular Weight 17 kDA
Comments

Background: Changes in chromatin structure play a large role in the regulation of transcription in eukaryotes (1). The nucleosome is the primary building block of chromatin, and is made up of four core histone proteins (H2A, H2B, H3 and H4) (2). Acetylation of core histones regulates gene expression (2). Histone H2B is primarily acetylated at lysines 5, 12, 15, and 20 (3). Ubiquitylation of Histone H2B at lysine 123 by Rad6 has been implicated in transcriptional activation and lysine methylation on histone H3 (4). Histone H2B phosphorylation at Ser10 (for yeast) and Ser14 (for mammalian cells) has been linked to regulation of apoptosis and meiosis (5).

Application Details
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Application Notes The suggested dilution is: WB: ~~ 1:1000~10000 ICC: ~~ 1:100 IHC: ~~ 1:100
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Storage Histone-H2B Antibody Acetyl (K15) can be stored at -20°C for up to 12 months from time of receipt.
Research Area Ubiquitin-related antibodies, Signaling, Phospho-specific antibodies, Protein Modifications, Transcription Factors, Chromatin, Cell Structure
Restrictions For Research Use only

Publications
Application Details Alternatives back to top

Product Braunstein, Sobel, Allis et al.: "Efficient transcriptional silencing in Saccharomyces cerevisiae requires a heterochromatin histone acetylation pattern." in: Molecular and cellular biology, Vol. 16, Issue 8, pp. 4349-56, 1996 (PubMed).

Workman, Kingston: "Alteration of nucleosome structure as a mechanism of transcriptional regulation." in: Annual review of biochemistry, Vol. 67, pp. 545-79, 1998 (PubMed).

Alternatives
Application Details Publications back to top

Alternatives for antigen "Histone H2B", type "Antibodies"
Hosts Rabbit (75), Sheep (4), Mouse (1)
Reactivities Human (72), Mouse (Murine) (40), Rat (Rattus) (29), Cow (Bovine) (23), Chicken (22), Dog (Canine) (20), Horse (Equine) (19), Pig (Porcine) (19), Mammalian (3), Fruit Fly (Drosophila melanogaster) (2), Zebrafish (Danio rerio) (2), Chimpanzee (1), Hamster (1), Yeast (Saccharomyces cerevisiae) (1)
Applications Western Blotting (WB) (59), Immunofluorescence (IF) (37), ELISA (29), Immunohistochemistry (IHC) (20), Immunoprecipitation (IP) (20), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (19), Immunocytochemistry (ICC) (12), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Flow Cytometry (FACS) (1), Immunoelectron Microscopy (IEM) (1)
Conjugates Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1)
Epitopes N-Term (7), acLys5 (6), acLys12 (4), acLys20 (4), acLys15 (3), AA 111-125 (2), AA 1-22 (1), AA 118-126 (1), C-Term (1), acLys12, acLys15, acLys20 (1), acLys16 (1), acetylated (1), pSer14 (1)