|+1 404 474 4654|
|+1 888 205 9894 (TF)|
Histone H2B (acLys15) antibody
|Synonyms||DmelCG17949, CG17949, H2B, FLJ35099, FLJ56780, FLJ56787, MGC131639, HISTONE 2B, HISTONE H2B, HTB9, HTB2, MRN17.11, MRN17_11|
Human, Rat (Rattus)
Alternatives Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
|2 references available|
|Price||507.14 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
|Description||Other names: HIST2H2BF, Histone H2B type 2-F|
|Characteristics||Rabbit Monoclonal Antibody|
|Specificity||A synthetic acetylated peptide corresponding to residues surrounding Lysine 15 of Histone H2B was used as an immunogen. The antibody only detects Histone H2B acetylated on Lysine 15.|
|Molecular Weight||17 kDA|
Background: Changes in chromatin structure play a large role in the regulation of transcription in eukaryotes (1). The nucleosome is the primary building block of chromatin, and is made up of four core histone proteins (H2A, H2B, H3 and H4) (2). Acetylation of core histones regulates gene expression (2). Histone H2B is primarily acetylated at lysines 5, 12, 15, and 20 (3). Ubiquitylation of Histone H2B at lysine 123 by Rad6 has been implicated in transcriptional activation and lysine methylation on histone H3 (4). Histone H2B phosphorylation at Ser10 (for yeast) and Ser14 (for mammalian cells) has been linked to regulation of apoptosis and meiosis (5).
|Application Notes||The suggested dilution is: WB: ~~ 1:1000~10000 ICC: ~~ 1:100 IHC: ~~ 1:100|
|Buffer||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Histone-H2B Antibody Acetyl (K15) can be stored at -20°C for up to 12 months from time of receipt.|
|Research Area||Ubiquitin-related antibodies, Signaling, Phospho-specific antibodies, Protein Modifications, Transcription Factors, Chromatin, Cell Structure|
|Restrictions||For Research Use only|
Braunstein, Sobel, Allis et al.: "Efficient transcriptional silencing in Saccharomyces cerevisiae requires a heterochromatin histone acetylation pattern." in: Molecular and cellular biology, Vol. 16, Issue 8, pp. 4349-56, 1996 (PubMed).
Workman, Kingston: "Alteration of nucleosome structure as a mechanism of transcriptional regulation." in: Annual review of biochemistry, Vol. 67, pp. 545-79, 1998 (PubMed).