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Histone H2B (acLys5) antibody

Details for Product No. ABIN649858
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Antigen
Synonyms CG17949, Dmel\\CG17949, H2B, HISTONE H2B, MRN17.11, MRN17_11, histone B2, CG33884, Dmel\CG33884, H2B.1, H2B/l, H2BFL, dJ221C16.3, 2610022J01Rik, H2bfs, R74621, Hist1h2bc, HISTONE 2B
Epitope
acLys5
(22), (21), (18), (11), (10), (8), (4), (3), (3), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human, Rat (Rattus)
(166), (101), (42), (39), (37), (27), (12), (3), (3), (2), (2), (2), (1), (1), (1), (1)
Host
Rabbit
(163), (5), (5)
Clonality
Monoclonal
Conjugate
Un-conjugated
(4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3)
Application
Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
(133), (82), (62), (58), (31), (30), (18), (18), (9), (1), (1)
Pubmed 2 references available
Quantity 100 µL
Options
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Catalog No. ABIN649858
507.14 $
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Specificity A synthetic acetylated peptide corresponding to residues surrounding Lys 5 of Histone H2B was used as an immunogen. The antibody only detects Histone H2B acetylated on Lys 5.
Alternative Name Histone-H2B
Background Changes in chromatin structure play a large role in the regulation of transcription in eukaryotes (1). The nucleosome is the primary building block of chromatin, and is made up of four core histone proteins (H2A, H2B, H3 and H4) (2). Acetylation of core histones regulates gene expression (2). Histone H2B is primarily acetylated at lysines 5, 12, 15, and 20 (3). Ubiquitylation of Histone H2B at lysine 123 by Rad6 has been implicated in transcriptional activation and lysine methylation on histone H3 (4). Histone H2B phosphorylation at Ser10 (for yeast) and Ser14 (for mammalian cells) has been linked to regulation of apoptosis and meiosis (5).
Synonyms: HIST2H2BF, Histone H2B type 2-F
Molecular Weight 14 kDA
Gene ID 440689
UniProt Q5QNW6
Research Area Ubiquitin-related antibodies, Signaling, Phospho-specific antibodies, Protein Modifications, Transcription Factors, Chromatin, Cell Structure
Application Notes The suggested dilution is: WB: = 1:50000- , IHC: = 1:250-500
Comment

Background: Changes in chromatin structure play a large role in the regulation of transcription in eukaryotes (1). The nucleosome is the primary building block of chromatin, and is made up of four core histone proteins (H2A, H2B, H3 and H4) (2). Acetylation of core histones regulates gene expression (2). Histone H2B is primarily acetylated at lysines 5, 12, 15, and 20 (3). Ubiquitylation of Histone H2B at lysine 123 by Rad6 has been implicated in transcriptional activation and lysine methylation on histone H3 (4). Histone H2B phosphorylation at Ser10 (for yeast) and Ser14 (for mammalian cells) has been linked to regulation of apoptosis and meiosis (5).

Restrictions For Research Use only
Format Liquid
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Histone-H2B Antibody Acetyl (K5) can be stored at -20°C for up to 12 months from time of receipt.
Expiry Date 12 months
Background publications Braunstein, Sobel, Allis et al.: "Efficient transcriptional silencing in Saccharomyces cerevisiae requires a heterochromatin histone acetylation pattern." in: Molecular and cellular biology, Vol. 16, Issue 8, pp. 4349-56, 1996 (PubMed).

Workman, Kingston: "Alteration of nucleosome structure as a mechanism of transcriptional regulation." in: Annual review of biochemistry, Vol. 67, pp. 545-79, 1998 (PubMed).

Validation Images
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