Histone Cluster 3, H3 (HIST3H3) (acLys18) antibody

Details for Product No. ABIN649859
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Antigen
Epitope
acLys18
(79), (56), (53), (51), (35), (31), (29), (27), (26), (26), (25), (24), (24), (22), (19), (18), (18), (16), (16), (15), (14), (13), (13), (13), (13), (12), (12), (12), (12), (12), (12), (12), (11), (11), (11), (11), (11), (10), (10), (9), (9), (9), (8), (8), (8), (7), (7), (5), (5), (5), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human, Rat (Rattus)
(962), (489), (474), (28), (19), (14), (7), (6), (5), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Host
Rabbit
(849), (128), (12), (4), (1)
Clonality
Monoclonal
Conjugate
Un-conjugated
(39), (32), (32), (23), (23), (23), (17), (16), (15), (15), (15), (15), (15), (15), (4)
Application
Immunohistochemistry (IHC), Western Blotting (WB)
(758), (309), (281), (271), (150), (130), (105), (93), (66), (50), (36), (22), (17), (16), (7), (3), (1), (1), (1)
Pubmed 2 references available
Quantity 100 μL
Options
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Specificity A synthetic acetylated peptide corresponding to residues surrounding Lys18 of Histone H3 was used as immunogen. The antibody only detects Histone H3 acetylated on Lysine 18.
Alternative Name Histone-H3 (HIST3H3 Antibody Abstract)
Background Changes in chromatin structure play a large role in the regulation of transcription in eukaryotes (1). The nucleosome is the primary building block of chromatin, and is made up of four core histone proteins (H2A, H2B, H3 and H4) (2). Acetylation of core histones regulates gene expression (2). Histone H3 is primarily acetylated at lysines 9, 14, 18, and 23 (3,4). Acetylation at lysine 9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms (3,4).
Synonyms: HIST2H2BF, Histone H2B type 2-F
Molecular Weight 17 kDA
Gene ID 8358, 8356, 8350
UniProt P68431
Research Area Ubiquitin-related antibodies, Signaling, Phospho-specific antibodies, Protein Modifications, Transcription Factors, Chromatin, Cell Structure
Application Notes IHC: = 1:100-250, WB: = 1:500
Comment

Background: Changes in chromatin structure play a large role in the regulation of transcription in eukaryotes (1). The nucleosome is the primary building block of chromatin, and is made up of four core histone proteins (H2A, H2B, H3 and H4) (2). Acetylation of core histones regulates gene expression (2). Histone H3 is primarily acetylated at lysines 9, 14, 18, and 23 (3,4). Acetylation at lysine 9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms (3,4).

Restrictions For Research Use only
Format Liquid
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Histone-H3 Antibody Acetyl (K18) can be stored at -20°C for up to 12 months from time of receipt.
Expiry Date 12 months
Background publications Workman, Kingston: "Alteration of nucleosome structure as a mechanism of transcriptional regulation." in: Annual review of biochemistry, Vol. 67, pp. 545-79, 1998 (PubMed).

Braunstein, Sobel, Allis et al.: "Efficient transcriptional silencing in Saccharomyces cerevisiae requires a heterochromatin histone acetylation pattern." in: Molecular and cellular biology, Vol. 16, Issue 8, pp. 4349-56, 1996 (PubMed).

Validation Images
Catalog No. ABIN649859
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