Histone Cluster 3, H3 (HIST3H3) (2meLys9) antibody

Details for Product No. ABIN649863
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Antigen
Synonyms H3.4, H3/g, H3FT, H3t
Epitope
2meLys9
(45), (43), (39), (30), (28), (25), (24), (23), (21), (21), (19), (19), (18), (17), (17), (16), (14), (11), (11), (9), (9), (8), (8), (7), (7), (7), (5), (5), (5), (5), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human, Rat (Rattus)
(629), (406), (399), (184), (169), (12), (7), (6), (4), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Host
Rabbit
(581), (73), (8), (2)
Clonality
Monoclonal
Conjugate
Un-conjugated
(18), (18), (18), (17), (17), (17), (17), (17), (17), (17), (17), (2)
Application
Western Blotting (WB)
(465), (217), (184), (169), (164), (86), (63), (59), (43), (27), (19), (8), (1)
Pubmed 2 references available
Catalog no. ABIN649863
Quantity 100 µL
Price
507.14 $   Plus shipping costs $45.00
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Specificity A synthetic (Dimethyl-K)-peptide corresponding to residues surrounding Lys9 of Histone H3 was used as immunogen. The antibody only detects Histone H3 dimethylated on Lysine 9. Predicted to cross-react with most species, based on sequence homology.
Alternative Name Histone-H3
Background Changes in chromatin structure play a large role in the regulation of transcription in eukaryotes (1). The nucleosome is the primary building block of chromatin, and is made up of four core histone proteins (H2A, H2B, H3 and H4) (2). Various post-translational modifications, such as acetylation and methylation, of core histones serve as regulators of gene expression (2). Histone H3 is primarily acetylated at lysines 9, 14, 18, and 23 (3,4). Methylation also serves as a predominant form of post-translational modification of Histone H3, and has been linked to transcriptional regulation and epigenetic silencing via heterochromatin assembly (5). Lysines 4, 9 and 27 in the N-terminal tail of Histone H3 are the preferred sites of methylation.
Synonyms: HIST3H3, H3FT, Histone H3.1t, H3/g
Molecular Weight 17 kDA
Gene ID 8290
UniProt Q16695
Research Area Ubiquitin-related antibodies, Signaling, Phospho-specific antibodies, Protein Modifications, Transcription Factors, Chromatin, Cell Structure
Application Notes The suggested dilution is: WB: = 1:1000
Comment

Background: Changes in chromatin structure play a large role in the regulation of transcription in eukaryotes (1). The nucleosome is the primary building block of chromatin, and is made up of four core histone proteins (H2A, H2B, H3 and H4) (2). Various post-translational modifications, such as acetylation and methylation, of core histones serve as regulators of gene expression (2). Histone H3 is primarily acetylated at lysines 9, 14, 18, and 23 (3,4). Methylation also serves as a predominant form of post-translational modification of Histone H3, and has been linked to transcriptional regulation and epigenetic silencing via heterochromatin assembly (5). Lysines 4, 9 and 27 in the N-terminal tail of Histone H3 are the preferred sites of methylation.

Restrictions For Research Use only
Format Liquid
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Preservative Sodium azide
Storage -20 °C
Storage Comment Histone-H3 Antibody Dimethyl (K9) can be stored at -20°C for up to 12 months from time of receipt.
Expiry Date 12 months
Background publications Braunstein, Sobel, Allis et al.: "Efficient transcriptional silencing in Saccharomyces cerevisiae requires a heterochromatin histone acetylation pattern." in: Molecular and cellular biology, Vol. 16, Issue 8, pp. 4349-56, 1996 (PubMed).

Workman, Kingston: "Alteration of nucleosome structure as a mechanism of transcriptional regulation." in: Annual review of biochemistry, Vol. 67, pp. 545-79, 1998 (PubMed).

Hosts (581), (73), (8), (2)
Reactivities (629), (406), (399), (184), (169), (12), (7), (6), (4), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Applications (465), (217), (184), (169), (164), (86), (63), (59), (43), (27), (19), (8), (1)
Conjugates (18), (18), (18), (17), (17), (17), (17), (17), (17), (17), (17), (2)
Epitopes (45), (43), (39), (30), (28), (25), (24), (23), (21), (21), (19), (19), (18), (17), (17), (16), (14), (11), (11), (9), (9), (8), (8), (7), (7), (7), (5), (5), (5), (5), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
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