Add to Basket Bulk discount
Order hotline:
+1 404 474 4654
+1 888 205 9894 (TF)

Histone H3 (2meLys9) antibody

Antigen

Histone H3
Synonyms
H3t, H3.4, H3/g, H3FT, MGC126886, MGC126888, Hist1, H3.2-221, DmelCG31613, CG31613, h3.2, h3/m, h3f2, h3fm, hist1h3g, H3F3, H3.3A, H3F3B, MGC87782, MGC87783, H3.3B, H3F3A, h3f3a, MGC52829, MGC86731, M ... show more
Binding Site
Alternatives

2meLys9
Clonality Monoclonal
Host
Alternatives

Rabbit
Reactivity
Alternatives

Human, Rat (Rattus)
Conjugate
Alternatives Un-conjugated
Application
Alternatives Western Blotting (WB), Immunocytochemistry (ICC)
2 references available
Certificates ISO 9001:2008
Catalog no. ABIN649863
Quantity 100ul
Price 507.14 $   Plus shipping costs $45.00
Bulk discount
Shipping to
Availability Will be delivered in 2 to 3 Business Days

Additional Information
Application Details Publications Alternatives back to top

Alternative name Histone-H3
Gene ID 8290
UniProt Q16695
Description Other names: HIST3H3, H3FT, Histone H3.1t, H3/g
Characteristics Rabbit Monoclonal Antibody
Specificity A synthetic (Dimethyl-K)-peptide corresponding to residues surrounding Lys9 of Histone H3 was used as immunogen. The antibody only detects Histone H3 dimethylated on Lysine 9. Predicted to cross-react with most species, based on sequence homology.
Molecular Weight 17 kDA
Comments

Background: Changes in chromatin structure play a large role in the regulation of transcription in eukaryotes (1). The nucleosome is the primary building block of chromatin, and is made up of four core histone proteins (H2A, H2B, H3 and H4) (2). Various post-translational modifications, such as acetylation and methylation, of core histones serve as regulators of gene expression (2). Histone H3 is primarily acetylated at lysines 9, 14, 18, and 23 (3,4). Methylation also serves as a predominant form of post-translational modification of Histone H3, and has been linked to transcriptional regulation and epigenetic silencing via heterochromatin assembly (5). Lysines 4, 9 and 27 in the N-terminal tail of Histone H3 are the preferred sites of methylation.
Synonyms H3t, H3.4, H3/g, H3FT, MGC126886, MGC126888, Hist1, H3.2-221, DmelCG31613, CG31613, h3.2, h3/m, h3f2, h3fm, hist1h3g, H3F3, H3.3A, H3F3B, MGC87782, MGC87783, H3.3B, H3F3A, h3f3a, MGC52829, MGC86731, MGC165454, zgc:56193, zgc:86731, wu:fb58e10, FLJ35099, FLJ56780, FLJ56787, MGC131639, MGC89877, HIST1H3A

Application Details
Publications Alternatives back to top

Application Notes The suggested dilution is: WB: ~~ 1:1000 ICC: ~~ 1:100
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Storage Histone-H3 Antibody Dimethyl (K9) can be stored at -20°C for up to 12 months from time of receipt.
Research Area Ubiquitin-related antibodies, Signaling, Phospho-specific antibodies, Protein Modifications, Transcription Factors, Chromatin, Cell Structure
Restrictions For Research Use only

Publications
Application Details Alternatives back to top

Product Braunstein, Sobel, Allis et al.: "Efficient transcriptional silencing in Saccharomyces cerevisiae requires a heterochromatin histone acetylation pattern." in: Molecular and cellular biology, Vol. 16, Issue 8, pp. 4349-56, 1996 (PubMed).

Workman, Kingston: "Alteration of nucleosome structure as a mechanism of transcriptional regulation." in: Annual review of biochemistry, Vol. 67, pp. 545-79, 1998 (PubMed).

Alternatives
Application Details Publications back to top

Alternatives for antigen "Histone H3", type "Antibodies"
Hosts Rabbit (453), Mouse (26), Sheep (6), Rat (1)
Reactivities Human (465), Rat (Rattus) (341), Mouse (Murine) (337), Cow (Bovine) (256), Pig (Porcine) (252), Fruit Fly (Drosophila melanogaster) (234), Rabbit (19), Dog (Canine) (18), Sheep (Ovine) (18), All Species (1), Arabidopsis thaliana (1), Hamster (1), Mammalian (1), Rice (Oryza sativa) (1), Tetrahymena (1), Yeast (1)
Applications Immunofluorescence (IF) (300), Western Blotting (WB) (204), ELISA (139), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (76), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (50), Immunohistochemistry (IHC) (45), Immunoprecipitation (IP) (35), Immunocytochemistry (ICC) (28), Immunoelectron Microscopy (IEM) (17), Flow Cytometry (FACS) (7), Immunohistochemistry (Frozen Sections) (IHC (fro)) (6), Dot Blot (DB) (1), Dot Blot (Dot) (1), Functional Studies (Func) (1)
Conjugates Alexa Fluor 488 (18), Alexa Fluor 350 (17), Alexa Fluor 555 (17), Alexa Fluor 647 (17), Biotin (17), Cy3 (17), Cy5 (17), Cy5.5 (17), Cy7 (17), FITC (17), Gold (17), HRP (17), PE (17), PE,Cy3 (16), PE,Cy5 (16), PE,Cy5.5 (16), PE,Cy7 (16), Agarose Beads (2), PE-Cy3 (1), PE-Cy5 (1), PE-Cy5.5 (1), PE-Cy7 (1)
Epitopes pSer28 (43), pSer10 (38), pThr3 (38), pThr11 (26), C-Term (25), 3meLys4 (22), acLys9 (22), 2meLys9 (21), acLys18 (21), 2meLys27 (20), 2meLys4 (20), N-Term (20), acLys23 (20), 3meLys36 (19), 2meLys36 (18), meLys79 (17), Lys27 (4), acLys14 (4), meLys9 (4), Lys4 (3), meLys4 (3), 2meArg2 (2), 3meLys27 (2), Lys36 (2), Lys9 (2), Mono Methyl Lysine 4 (2), Tri Methyl Lysine 4 (2), acLys27 (2), acLys56 (2), acetylated (2), ciArg2, ciArg8, ciArg17 (2), 2meArg8 (1), 2meLys3 (1), 2meLys4, 2meLys9 (1), 3meLys9 (1), AA 1-30 (1), AA 66-82 (1), AA 7-20 (1), Arg2 (1), Lys14,Lys9 (1), Lys18 (1), Lys79 (1), N-Term,AA 1-30 (1), N-Term,Lys9 (1), Ser10 (1), ciArg2,ciArg8,ciArg17 (1), meLys9, 2meLys9, 3meLys9 (1), pSer10,pSer28 (1)