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Histone H3.3 (pSer28), (pSer31) antibody

Details for Product No. ABIN649864
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Antigen
Synonyms
CG8989, Dmel\\CG8989, H3.3, H3.3B, His3.3, HisH3-3Q, PH3, anon-EST:fe1B6, dH3.3B, h3f3a, h3f3c, h3f3d, wu:fa25h06, wu:fa96g06, wu:fb07a08, wu:fb36f01, zgc:110292, zgc:174300, H3F3A, 9430068D06Rik, H3- ... show more
CG8989, Dmel\\CG8989, H3.3, H3.3B, His3.3, HisH3-3Q, PH3, anon-EST:fe1B6, dH3.3B, h3f3a, h3f3c, h3f3d, wu:fa25h06, wu:fa96g06, wu:fb07a08, wu:fb36f01, zgc:110292, zgc:174300, H3F3A, 9430068D06Rik, H3-IX, H3-X, H3F3C, CG5825, Dmel\\CG5825, H3.3A, His-3.3A, dH3.3A show less
Epitope
pSer28, pSer31
(7), (2), (1), (1), (1), (1)
Reactivity
Human
(43), (14), (13), (2)
Host
Rabbit
(39), (6)
Clonality
Monoclonal
Conjugate
Un-conjugated
(3), (3), (3)
Application
Western Blotting (WB)
(37), (27), (16), (11), (9), (9), (3), (3)
Pubmed 2 references available
Quantity 100 µL
Options
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Catalog No. ABIN649864
507.14 $
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Specificity A synthetic phospho-peptide corresponding to residues surrounding serine 28 and serine 31 of human Histone H3.3 protein.
Alternative Name Histone-H3.3
Background Changes in chromatin structure play a large role in the regulation of transcription in eukaryotes (1). The nucleosome is the primary building block of chromatin, and is made up of four core histone proteins (H2A, H2B, H3 and H4) (2). Various post-translational modifications, such as acetylation and methylation, of core histones serve as regulators of gene expression (2). Histone H3.3 is a variant histone which replaces conventional H3 in nucleosomes in active genes. It is predominant from of histone H3 in non-dividing cells and it is incorporated into chromatin independently of DNA synthesis. H3.3 is phosphorylated at serine 28 by MLTK isoform 1, RPS6KA5, and AURKB during mitosis. Also, phosphorylation at serine 31 on H3.3 is specific to regions bordering centromeres in metaphase chromosomes.
Synonyms: H3f3b, H3.3b, Histone H3.3
Molecular Weight 17 kDA
Gene ID 3020, 3021
UniProt P84243
Research Area Ubiquitin-related antibodies, Signaling, Phospho-specific antibodies, Protein Modifications, Transcription Factors, Chromatin, Cell Structure
Application Notes The suggested dilution is: WB: = 1:2000
Comment

Background: Changes in chromatin structure play a large role in the regulation of transcription in eukaryotes (1). The nucleosome is the primary building block of chromatin, and is made up of four core histone proteins (H2A, H2B, H3 and H4) (2). Various post-translational modifications, such as acetylation and methylation, of core histones serve as regulators of gene expression (2). Histone H3.3 is a variant histone which replaces conventional H3 in nucleosomes in active genes. It is predominant from of histone H3 in non-dividing cells and it is incorporated into chromatin independently of DNA synthesis. H3.3 is phosphorylated at serine 28 by MLTK isoform 1, RPS6KA5, and AURKB during mitosis. Also, phosphorylation at serine 31 on H3.3 is specific to regions bordering centromeres in metaphase chromosomes.

Restrictions For Research Use only
Format Liquid
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Histone-H3.3 Antibody Phospho-pS28&31 can be stored at -20°C for up to 12 months from time of receipt.
Expiry Date 12 months
Background publications Braunstein, Sobel, Allis et al.: "Efficient transcriptional silencing in Saccharomyces cerevisiae requires a heterochromatin histone acetylation pattern." in: Molecular and cellular biology, Vol. 16, Issue 8, pp. 4349-56, 1996 (PubMed).

Workman, Kingston: "Alteration of nucleosome structure as a mechanism of transcriptional regulation." in: Annual review of biochemistry, Vol. 67, pp. 545-79, 1998 (PubMed).

Validation Images
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