Histone-H3T (acLys27) antibody

Details for Product No. ABIN649865
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Antigen
Epitope
acLys27
Reactivity
Human, Rat (Rattus)
Host
Rabbit
Clonality
Monoclonal
Application
Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Pubmed 2 references available
Catalog no. ABIN649865
Quantity 100 µL
Price
507.14 $   Plus shipping costs $45.00
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Specificity A synthetic acetylated peptide corresponding to residues surrounding Lys 27 of Histone H3T was used as immunogen. The antibody only detects Histone H3T acetylated on Lysine 27.
Background Changes in chromatin structure play a large role in the regulation of transcription in eukaryotes (1). The nucleosome is the primary building block of chromatin, and is made up of four core histone proteins (H2A, H2B, H3 and H4) (2). Acetylation of core histones regulates gene expression (2). Histone H3 is primarily acetylated at lysines 9, 14, 18, and 23 (3,4). Acetylation at lysine 9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms (3,4). . H3t (H.34) differs from the consensus mammalian H3 structure by four amino acid residues and it is mainly expressed in testicular cells. H3t encoding gene maps to chromosome 1 (1q42), unlike the majority of the human histone genes that form cluster on chromosome 6 and outside of replication-dependent H3 genes (5).
Synonyms: HIST2H2BF, Histone H2B type 2-F
Molecular Weight 17 kDA
Gene ID 8356, 8352, 8354
UniProt P68431
Research Area Ubiquitin-related antibodies, Signaling, Phospho-specific antibodies, Protein Modifications, Transcription Factors, Chromatin, Cell Structure
Application Notes The suggested dilution is: WB: = 1:500 , IHC: = 1:100-250
Comment

Background: Changes in chromatin structure play a large role in the regulation of transcription in eukaryotes (1). The nucleosome is the primary building block of chromatin, and is made up of four core histone proteins (H2A, H2B, H3 and H4) (2). Acetylation of core histones regulates gene expression (2). Histone H3 is primarily acetylated at lysines 9, 14, 18, and 23 (3,4). Acetylation at lysine 9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms (3,4). . H3t (H.34) differs from the consensus mammalian H3 structure by four amino acid residues and it is mainly expressed in testicular cells. H3t encoding gene maps to chromosome 1 (1q42), unlike the majority of the human histone genes that form cluster on chromosome 6 and outside of replication-dependent H3 genes (5).

Restrictions For Research Use only
Format Liquid
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Preservative Sodium azide
Storage -20 °C
Storage Comment Histone-H3T Antibody Acetyl (K27) can be stored at -20°C for up to 12 months from time of receipt.
Expiry Date 12 months
Background publications Braunstein, Sobel, Allis et al.: "Efficient transcriptional silencing in Saccharomyces cerevisiae requires a heterochromatin histone acetylation pattern." in: Molecular and cellular biology, Vol. 16, Issue 8, pp. 4349-56, 1996 (PubMed).

Workman, Kingston: "Alteration of nucleosome structure as a mechanism of transcriptional regulation." in: Annual review of biochemistry, Vol. 67, pp. 545-79, 1998 (PubMed).

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