Heat Shock Protein 2 (HSPB2) antibody

Antigen: Heat Shock Protein 2 (HSPB2)

Clonality: Monoclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Certificates: ISO 9001:2008

Catalog no.: ABIN649891

Additional Information

Alternative name: HSP27

Gene ID: 3315

UniProt: P04792

Description: Other names: HSPB1, HSP27, HSP28, Heat shock protein beta-1, Estrogen-regulated 24 kDa protein,28 kDa heat shock protein

Characteristics: Rabbit Monoclonal Antibody

Specificity: A synthetic peptide corresponding to residues near the N-terminus of human Hsp 27 was used as an immunogen.

Molecular Weight: 27 kDA

Comments:

Background: Heat shock protein (HSP) 27 is one of the small HSPs, and is involved in stress resistance and actin organization. HSP27 is expressed in response to environmental stresses such as heat shock, or estrogen stimulation in MCF-7 cells (1,2). HSP27 is phosphorylated at serines 15, 78 and 82 by MAPKAP kinase 2 as a result of the activation of the p38 MAP kinase pathway (3,4).

Application Details

Application Notes: The suggested dilution is: WB: ~~ 1:1000~10000 ICC: ~~ 1:50 IHC: ~~ 1:100

Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage: HSP27 Antibody can be stored at -20°C for up to 12 months from time of receipt.

Research Area: Signaling, Phospho-specific antibodies, Cell Signaling, Protein Modifications, Neurology, Cell Structure

Restrictions: For Research Use only

Publications

Product: Rouse, Cohen, Trigon et al.: "A novel kinase cascade triggered by stress and heat shock that stimulates MAPKAP kinase-2 and phosphorylation of the small heat shock proteins." in: Cell, Vol. 78, Issue 6, pp. 1027-37, 1994 (PubMed).