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Details for Product No. ABIN649892

Heat Shock 27kDa Protein 1 (HSPB1) (pSer15) antibody

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Antigen
Synonyms CMT2F, HMN2B, HS.76067, HSP27, HSP28, Hsp25, SRP27, 27kDa, Hsp27, HSPB1, cb153, cb660, hsp1, hsp25, hsp27, id:ibd2821, sb:cb660, zgc:103437, cmt2f, hs.76067, hsp28
Epitope
»Alternatives pSer15
Reactivity
»Alternatives Human
Host
»Alternatives Rabbit
Clonality Monoclonal
Conjugate
»Alternatives Un-conjugated
Application
»Alternatives Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)
Pubmed 1 reference available
Catalog no. ABIN649892
Quantity 100 µL
Price
507.14 $   Plus shipping costs $45.00
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Availability Will be delivered in 2 to 3 Business Days
Specificity A synthetic phospho-peptide corresponding to residues surrounding Serine 15 of human HSP27 was used as immunogen. The antibody will detect HSP27 phosphorylation on Serine 15.
Alternative Name HSP27
Background Heat shock protein (HSP) 27 is one of the small HSPs, and is involved in stress resistance and actin organization. HSP27 is expressed in response to environmental stresses such as heat shock, or estrogen stimulation in MCF-7 cells (1,2). HSP27 is phosphorylated at serines 15, 78 and 82 by MAPKAP kinase 2 as a result of the activation of the p38 MAP kinase pathway (3,4).
Synonyms: HSPB1, HSP27, HSP28, Heat shock protein beta-1, Estrogen-regulated 24 kDa protein,28 kDa heat shock protein
Molecular Weight 27 kDA
Gene ID 3315
UniProt P04792
Research Area Signaling, Phospho-specific antibodies, Cell Signaling, Protein Modifications, Neurology, Cell Structure
Application Notes The suggested dilution is: WB: ~~ 1:2000 IP: ~~ 1:20 IHC: ~~ 1:100
Comment

Background: Heat shock protein (HSP) 27 is one of the small HSPs, and is involved in stress resistance and actin organization. HSP27 is expressed in response to environmental stresses such as heat shock, or estrogen stimulation in MCF-7 cells (1,2). HSP27 is phosphorylated at serines 15, 78 and 82 by MAPKAP kinase 2 as a result of the activation of the p38 MAP kinase pathway (3,4).

Restrictions For Research Use only
Format Liquid
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Preservative Sodium azide
Storage -20 °C
Storage Comment HSP27 Antibody Phospho (pS15) can be stored at -20°C for up to 12 months from time of receipt.
Expiry Date 12 months
Background publications Rouse, Cohen, Trigon et al.: "A novel kinase cascade triggered by stress and heat shock that stimulates MAPKAP kinase-2 and phosphorylation of the small heat shock proteins." in: Cell, Vol. 78, Issue 6, pp. 1027-37, 1994 (PubMed).

Alternatives for antigen "Heat Shock 27kDa Protein 1 (HSPB1)", type "Antibodies"
Hosts (300), (120), (3)
Reactivities (403), (110), (94), (63), (50), (25), (10), (10), (8), (7), (5), (4), (3), (2), (2), (1), (1), (1), (1)
Applications (340), (154), (124), (116), (77), (71), (47), (40), (23), (20), (5), (5), (3), (3), (2), (2), (2), (1), (1), (1)
Conjugates (17), (14), (4), (4), (4), (4), (4), (4), (4), (4), (4), (2), (2), (1), (1)
Epitopes (52), (49), (38), (12), (9), (8), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
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