Heat Shock 27kDa Protein 1 (HSPB1) (pSer78) antibody

Details for Product No. ABIN649893
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Antigen
Epitope
pSer78
(76), (66), (56), (25), (16), (16), (13), (12), (12), (12), (8), (8), (5), (5), (4), (4), (4), (4), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(630), (123), (119), (33), (30), (27), (25), (18), (17), (17), (17), (9), (4), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Host
Rabbit
(482), (182), (1)
Clonality
Monoclonal
Conjugate
Un-conjugated
(29), (28), (17), (13), (13), (12), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (2), (2), (2)
Application
Dot Blot (DB), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
(548), (258), (232), (141), (125), (110), (96), (40), (34), (25), (17), (16), (12), (5), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Pubmed 2 references available
Quantity 100 μL
Options
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Request Want additional data for this product?

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Specificity A synthetic phospho-peptide corresponding to residues surrounding Serine 78 of human HSP27 was used as immunogen. The antibody will detect Src phosphorylation on Serine 78.
Alternative Name HSP27 (HSPB1 Antibody Abstract)
Background Heat shock protein (HSP) 27 is one of the small HSPs, and is involved in stress resistance and actin organization. HSP27 is expressed in response to environmental stresses such as heat shock, or estrogen stimulation in MCF-7 cells (1,2). HSP27 is phosphorylated at serines 15, 78 and 82 by MAPKAP kinase 2 as a result of the activation of the p38 MAP kinase pathway (3,4).
Synonyms: HSPB1, HSP27, HSP28, Heat shock protein beta-1, Estrogen-regulated 24 kDa protein,28 kDa heat shock protein
Molecular Weight 27 kDA
Gene ID 3315
UniProt P04792
Research Area Signaling, Phospho-specific antibodies, Cell Signaling, Protein Modifications, Neurology, Cell Structure
Pathways MAPK Pathway
Application Notes The suggested dilution is: WB: = 1:2000 , IHC: = 1:250-500
Comment

Background: Heat shock protein (HSP) 27 is one of the small HSPs, and is involved in stress resistance and actin organization. HSP27 is expressed in response to environmental stresses such as heat shock, or estrogen stimulation in MCF-7 cells (1,2). HSP27 is phosphorylated at serines 15, 78 and 82 by MAPKAP kinase 2 as a result of the activation of the p38 MAP kinase pathway (3,4).

Restrictions For Research Use only
Format Liquid
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment HSP27 Antibody Phospho (pS78) can be stored at -20°C for up to 12 months from time of receipt.
Expiry Date 12 months
Background publications Rouse, Cohen, Trigon et al.: "A novel kinase cascade triggered by stress and heat shock that stimulates MAPKAP kinase-2 and phosphorylation of the small heat shock proteins." in: Cell, Vol. 78, Issue 6, pp. 1027-37, 1994 (PubMed).

Mendelsohn, Zhu, ONeill: "The 29-kDa proteins phosphorylated in thrombin-activated human platelets are forms of the estrogen receptor-related 27-kDa heat shock protein." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 88, Issue 24, pp. 11212-6, 1992 (PubMed).

Validation Images
Catalog No. ABIN649893
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