Heat Shock Protein 2 (HSPB2) (pSer82) antibody

Antigen: Heat Shock Protein 2 (HSPB2)

Binding Site: pSer82

Clonality: Monoclonal

Host: Rabbit

Reactivity: Human, Rat (Rattus)

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Certificates: ISO 9001:2008

Catalog no.: ABIN649894

Additional Information

Alternative name: HSP27

Gene ID: 3315

UniProt: P04792

Description: Other names: HSPB1, HSP27, HSP28, Heat shock protein beta-1, Estrogen-regulated 24 kDa protein,28 kDa heat shock protein

Characteristics: Rabbit Monoclonal Antibody

Specificity: A synthetic phospho-peptide corresponding to residues surrounding Ser82 of human Hsp27 was used as immunogen. The antibody only detects Hsp27 phosphorylated on Serine 82.

Molecular Weight: 27 kDA

Comments:

Background: Heat shock protein (HSP) 27 is one of the small HSPs, and is involved in stress resistance and actin organization. HSP27 is expressed in response to environmental stresses such as heat shock, or estrogen stimulation in MCF-7 cells (1,2). HSP27 is phosphorylated at serines 15, 78 and 82 by MAPKAP kinase 2 as a result of the activation of the p38 MAP kinase pathway (3,4).

Application Details

Application Notes: The suggested dilution is: WB: ~~ 1:500 IP: ~~ 1:30 ICC: ~~ 1:100 IHC: ~~ 1:250

Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage: HSP27 Antibody Phospho (pS82) can be stored at -20°C for up to 12 months from time of receipt.

Research Area: Signaling, Phospho-specific antibodies, Cell Signaling, Protein Modifications, Neurology, Cell Structure

Restrictions: For Research Use only

Publications

Product: Mendelsohn, Zhu, ONeill: "The 29-kDa proteins phosphorylated in thrombin-activated human platelets are forms of the estrogen receptor-related 27-kDa heat shock protein." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 88, Issue 24, pp. 11212-6, 1992 (PubMed).

Rouse, Cohen, Trigon et al.: "A novel kinase cascade triggered by stress and heat shock that stimulates MAPKAP kinase-2 and phosphorylation of the small heat shock proteins." in: Cell, Vol. 78, Issue 6, pp. 1027-37, 1994 (PubMed).