HtrA Serine Peptidase 2 (HTRA2) (N-Term) antibody

Antigen: HtrA Serine Peptidase 2 (HTRA2)

Synonyms: OMI, PARK13, PRSS25, Omi, mnd2, Prss25, AI481710, HTRA2

Binding Site: N-Term

Clonality: Monoclonal

Host: Rabbit

Reactivity: Human, Rat (Rattus)

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Certificates: ISO 9001:2008

Catalog no.: ABIN649902

Additional Information

Alternative name: HtrA2

Gene ID: 27429

UniProt: O43464

Description: Other names: HTRA2, OMI, PRSS25, Serine protease HTRA2, mitochondrial, Omi stress-regulated endoprotease,Serine proteinase OMI,Serine protease 25

Characteristics: Rabbit Monoclonal Antibody

Specificity: A synthetic peptide corresponding to N-terminal residues of mature form of human HtrA2 was used as immunogen. The antibody does not cross-react with other peptidase S1C family members.

Molecular Weight: 38 kDA

Comments:

Background: HtrA2 (Omi) is a serine protease that plays a significant role in programmed cell death, or apoptosis. Localized to the intermembrane space of the mitochondria, HtrA2 exists predominantly as two isoforms (1). In response to cellular stress signals, HtrA2 is released into the cytosol. One way that HtrA2 induces apoptosis is by binding to BIRC proteins (also called inhibitor of apoptosis proteins, IAPs), and thereby inhibiting their caspase-inhibitory activity (2,3).

Application Details

Application Notes: The suggested dilution is: WB: ~~ 1:500~2000 IP: ~~ 1:40 IHC: ~~ 1:250

Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage: HtrA2 Antibody (N-term) can be stored at -20°C for up to 12 months from time of receipt.

Research Area: Apoptosis/Necrosis, Cell Signaling, Cell Structure, Metabolism, Neurology

Restrictions: For Research Use only

Publications

Product: Savopoulos, Carter, Turconi et al.: "Expression, purification, and functional analysis of the human serine protease HtrA2." in: Protein expression and purification, Vol. 19, Issue 2, pp. 227-34, 2000 (PubMed).

Gray, Ward, Karran et al.: "Characterization of human HtrA2, a novel serine protease involved in the mammalian cellular stress response." in: European journal of biochemistry / FEBS, Vol. 267, Issue 18, pp. 5699-710, 2000 (PubMed).

Suzuki, Imai, Nakayama et al.: "A serine protease, HtrA2, is released from the mitochondria and interacts with XIAP, inducing cell death." in: Molecular cell, Vol. 8, Issue 3, pp. 613-21, 2001 (PubMed).