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Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha (NFKBIA) antibody

Antigen

Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha (NFKBIA)
Synonyms IKBA, MAD-3, NFKBI
Clonality Monoclonal
Host
Alternatives

Rabbit
Reactivity
Alternatives

Human, Rat (Rattus)
Conjugate
Alternatives Un-conjugated
Application
Alternatives Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FACS)
1 reference available
Certificates ISO 9001:2008
Catalog no. ABIN649908
Quantity 100ul  (Variants)
Price 450.00 $   Plus shipping costs $45.00
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Additional Information
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Alternative name IkappaB-alpha
Gene ID 4792
UniProt P25963
Description Other names: NFKBIA, IKBA, MAD3, NFKBI, NF-kappa-B inhibitor alpha, Major histocompatibility complex enhancer-binding protein MAD3
Characteristics Rabbit Monoclonal Antibody
Specificity A synthetic peptide corresponding to residues on the C terminus of human IkappaB-alpha was used as an immunogen.
Molecular Weight 37 kDA
Comments

Background: The NF-kappaB/Rel transcription factors are present in the cytosol in an inactive state, complexed with the inhibitory IkappaB-alpha protein (1-4). In response to many different NF-kappaB-inducing agents including T-cell mitogens, proinflammatory cytokines, and viral transactivators the inhibitory IkappaB-alpha is rapidly phosphorylated and degraded (2). Phosphorylation of IkappaB-alpha at Ser32 and Ser36 has been shown to stimulate conjugation with ubiquitin and subsequent degradation of IkappaB-alpha (3). Phosphorylation of IkappaB-alpha at Ser32 and Ser36 is critical for activation of NF-kappaB, which then translocates to the nucleus and induces transcription of genes that protect organism (1-4).

Application Details
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Application Notes The suggested dilution is: WB: ~~ 1:1000~10000 IP: ~~ 1:10 ICC: ~~ 1:50~100 IHC: ~~ 1:100~250
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Storage IkappaB-alpha Antibody can be stored at -20°C for up to 12 months from time of receipt.
Research Area Ubiquitin-related antibodies, Phospho-specific antibodies, Protein Modifications, Cell Structure
Restrictions For Research Use only

Publications
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Product Brockman, Scherer, McKinsey et al.: "Coupling of a signal response domain in I kappa B alpha to multiple pathways for NF-kappa B activation." in: Molecular and cellular biology, Vol. 15, Issue 5, pp. 2809-18, 1995 (PubMed).

Alternatives
Application Details Publications Variants back to top

Alternatives for antigen "Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha (NFKBIA)", type "Antibodies"
Hosts Rabbit (148), Mouse (26), Sheep (4), Goat (1)
Reactivities Human (171), Mouse (Murine) (94), Rat (Rattus) (79), Cow (Bovine) (20), Pig (Porcine) (20), Sheep (Ovine) (15), Dog (Canine) (9), Chicken (7), Cat (Feline) (1), Hamster (1), Rabbit (1)
Applications Western Blotting (WB) (116), ELISA (66), Immunohistochemistry (IHC) (56), Immunofluorescence (IF) (51), Immunoprecipitation (IP) (37), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (31), Immunocytochemistry (ICC) (8), Gel Shift (GS) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Immunohistochemistry (Frozen Sections) (IHC (fro)) (3), Dot Blot (DB) (2), Dot Blot (Dot) (2), Flow Cytometry (FACS) (2), Electrophoretic Mobility-Shift Assay (EMSA) (1), Immunoelectron Microscopy (IEM) (1), Immunohistochemistry (Fixed) (IHC (fx)) (1)
Conjugates Biotin (8), Alexa Fluor 350 (4), Alexa Fluor 488 (4), Alexa Fluor 555 (4), Alexa Fluor 647 (4), PE,Cy5.5 (4), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy7 (1)
Epitopes pTyr42 (14), pSer32, pSer36 (13), C-Term (7), N-Term (5), pTyr305 (5), pSer36,pSer32 (4), AA 32-291 (3), Ser32, Ser36 (3), AA 34-48 (2), Pser32,pSer36 (2), Ser32 (2), Ser32,Ser36 (2), Tyr181 (2), Tyr42 (2), AA 130-143 (1), AA 32-36 (1), AA 32-47 (1), Internal Region (1), Ser23 (1), pSer32 (1), pSer32,pSer36 (1), pSer320 (1), pSer36 (1), pSer893 (1)