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Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha (NFKBIA) (pSer320) antibody

Details for Product No. ABIN649910
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Antigen
Synonyms IKBA, MAD-3, NFKBI, AI462015, Nfkbi, RL/IF-1, ECI-6/IKBA
Epitope
pSer320
(42), (39), (31), (29), (24), (16), (14), (7), (7), (7), (6), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(286), (166), (145), (41), (38), (14), (14)
Host
Rabbit
(261), (32), (5)
Clonality
Monoclonal
Conjugate
Un-conjugated
(9), (7), (7), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3)
Application
Western Blotting (WB)
(217), (117), (89), (53), (40), (39), (38), (12), (11), (8), (4), (3), (3), (2), (1)
Pubmed 5 references available
Quantity 100 µL
Options
Shipping to United States (Change)
Availability Will be delivered in 2 to 3 Business Days
Catalog No. ABIN649910
507.14 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Specificity A phospho specific peptide corresponding to residues surrounding Serine 32 of human IkappaB-alpha was used as an immunogen.
Alternative Name IkappaB-alpha
Background The NF-kappaB/Rel transcription factors are present in the cytosol in an inactive state, complexed with the inhibitory IkappaB-alpha protein (1-5). In response to many different NF-kappaB-inducing agents including T-cell mitogens, proinflammatory cytokines, and viral transactivators, the inhibitory IkappaB-alpha is rapidly phosphorylated and degraded (2). Phosphorylation of IkappaB-alpha at Ser32 and Ser36 has been shown to stimulate conjugation with ubiquitin and subsequent degradation of IkappaB-alpha (3). Activation of NF-kappaB can also be achieved after tyrosine phosphorylation of IkappaBalpha at tyrosine 42, an event that also ultimately leads to the dissociation of NF-kappaB and IkappaBalpha (4). Phosphorylation of IkappaB-alpha at Ser32 and Tyr42 is critical for activation of NF-kappaB, which then translocates to the nucleus and induces transcription of genes that protect organism (1-5).
Molecular Weight 40 kDA
Gene ID 6709
UniProt P25963
Research Area Ubiquitin-related antibodies, Phospho-specific antibodies, Protein Modifications, Cell Structure
Application Notes The suggested dilution is: WB: = 1:1000-10000
Comment

Background: The NF-kappaB/Rel transcription factors are present in the cytosol in an inactive state, complexed with the inhibitory IkappaB-alpha protein (1-5). In response to many different NF-kappaB-inducing agents including T-cell mitogens, proinflammatory cytokines, and viral transactivators, the inhibitory IkappaB-alpha is rapidly phosphorylated and degraded (2). Phosphorylation of IkappaB-alpha at Ser32 and Ser36 has been shown to stimulate conjugation with ubiquitin and subsequent degradation of IkappaB-alpha (3). Activation of NF-kappaB can also be achieved after tyrosine phosphorylation of IkappaBalpha at tyrosine 42, an event that also ultimately leads to the dissociation of NF-kappaB and IkappaBalpha (4). Phosphorylation of IkappaB-alpha at Ser32 and Tyr42 is critical for activation of NF-kappaB, which then translocates to the nucleus and induces transcription of genes that protect organism (1-5).

Restrictions For Research Use only
Format Liquid
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment IkappaB-alpha Antibody Phospho (S32) can be stored at -20°C for up to 12 months from time of receipt.
Expiry Date 12 months
Background publications Brockman, Scherer, McKinsey et al.: "Coupling of a signal response domain in I kappa B alpha to multiple pathways for NF-kappa B activation." in: Molecular and cellular biology, Vol. 15, Issue 5, pp. 2809-18, 1995 (PubMed).

Traenckner, Pahl, Henkel et al.: "Phosphorylation of human I kappa B-alpha on serines 32 and 36 controls I kappa B-alpha proteolysis and NF-kappa B activation in response to diverse stimuli." in: The EMBO journal, Vol. 14, Issue 12, pp. 2876-83, 1995 (PubMed).

Brown, Gerstberger, Carlson et al.: "Control of I kappa B-alpha proteolysis by site-specific, signal-induced phosphorylation." in: Science (New York, N.Y.), Vol. 267, Issue 5203, pp. 1485-8, 1995 (PubMed).

Chen, Parent, Maniatis: "Site-specific phosphorylation of IkappaBalpha by a novel ubiquitination-dependent protein kinase activity." in: Cell, Vol. 84, Issue 6, pp. 853-62, 1996 (PubMed).

Witherow, Garrison, Miller et al.: "beta-Arrestin inhibits NF-kappaB activity by means of its interaction with the NF-kappaB inhibitor IkappaBalpha." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 23, pp. 8603-7, 2004 (PubMed).

Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Catalog No. ABIN649910
507.14 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
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