conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK) (N-Term) antibody

Antigen: conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK)

Synonyms: IKK1, IKKA, IKBKA, TCF16, NFKBIKA, IKK-alpha, Ikka, Chuk1, Fbx24, Fbxo24, AI256658, MGC25325, CHUK, chuk, MGC83432, DKFZp459O053

Binding Site: N-Term

Clonality: Monoclonal

Host: Rabbit

Reactivity: Human, Rat (Rattus)

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunoprecipitation (IP)

Certificates: ISO 9001:2008

Catalog no.: ABIN649911

Additional Information

Alternative name: IKK alpha

Gene ID: 1147

UniProt: O15111

Description: Other names: CHUK, IKKA, TCF16, Inhibitor of nuclear factor kappa-B kinase subunit alpha, Conserved helix-loop-helix ubiquitous kinase,Nuclear factor NF-kappa-B inhibitor kinase alpha

Characteristics: Rabbit Monoclonal Antibody

Specificity: A synthetic peptide corresponding to residues in the N-term of human IKK-alpha was used as immunogen.

Molecular Weight: 85 kDA

Comments:

Background: IKK-alpha (I-Kappa-B kinase-alpha) is a member of the IKK complex which is composed of IKK-alpha, IKK-beta, IKK-gamma and IKAP (1-2). Phosphorylation of I-Kappa-B on a serine residue by the IKK complex frees NF-kB from I-Kappa-B and marks it for degradation via ubiquination. Activation of NFKB by TNF requires phosphorylation of IKK-alpha at threonine 23 and serine 176 by AKT1 and NIK respectively (3). It has been demonstrated that IKK-alpha can accumulate in the nucleus after cytokine exposure (4). In the nucleus IKK-alpha interacts with CREB-binding protein in conjunction with RELA to be recruited to the NF-kappa-B-responsive promoters in order to mediate the cytokine-induced phosphorylation and subsequent acetylation of specific residues in histone H3 (5).

Application Details

Application Notes: The suggested dilution is: WB: ~~ 1:1000 IP: ~~ 1:10 FC: ~~ 1:50 ICC: ~~ 1:500 IHC: ~~ 1:50

Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage: IKK alpha Antibody (N-term) can be stored at -20°C for up to 12 months from time of receipt.

Research Area: Signaling, Phospho-specific antibodies, Cell Signaling, Protein Modifications, Chromatin, Cell Structure

Restrictions: For Research Use only

Publications

Product: Zandi, Rothwarf, Delhase et al.: "The IkappaB kinase complex (IKK) contains two kinase subunits, IKKalpha and IKKbeta, necessary for IkappaB phosphorylation and NF-kappaB activation." in: Cell, Vol. 91, Issue 2, pp. 243-52, 1997 (PubMed).

Scheidereit: "Signal transduction. Docking IkappaB kinases." in: Nature, Vol. 395, Issue 6699, pp. 225-6, 1998 (PubMed).

Ozes, Mayo, Gustin et al.: "NF-kappaB activation by tumour necrosis factor requires the Akt serine-threonine kinase." in: Nature, Vol. 401, Issue 6748, pp. 82-5, 1999 (PubMed).

Yamamoto, Verma, Prajapati et al.: "Histone H3 phosphorylation by IKK-alpha is critical for cytokine-induced gene expression." in: Nature, Vol. 423, Issue 6940, pp. 655-9, 2003 (PubMed).

Anest, Hanson, Cogswell et al.: "A nucleosomal function for IkappaB kinase-alpha in NF-kappaB-dependent gene expression." in: Nature, Vol. 423, Issue 6940, pp. 659-63, 2003 (PubMed).