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conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK) (N-Term) antibody

Details for Product No. ABIN649911
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Antigen
Synonyms ikk, ikk1, ikka, ikkalpha, CHUK, IKBKA, IKK-alpha, IKK1, IKKA, NFKBIKA, TCF16, AI256658, Chuk1, Fbx24, Fbxo24, Ikka, zgc:56539
Epitope
N-Term
(35), (28), (22), (14), (12), (11), (10), (10), (8), (8), (8), (7), (7), (7), (7), (6), (5), (4), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Reactivity
Human, Rat (Rattus)
(259), (99), (91), (48), (24), (24), (24), (12), (1)
Host
Rabbit
(220), (42), (2), (1)
Clonality
Monoclonal
Conjugate
Un-conjugated
(10), (8), (8), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4)
Application
Western Blotting (WB), Immunohistochemistry (IHC)
(219), (109), (55), (43), (40), (39), (30), (26), (6), (3), (2), (1), (1)
Pubmed 5 references available
Quantity 100 µL
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Catalog No. ABIN649911
450.00 $
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Specificity A synthetic peptide corresponding to residues in the N-term of human IKK-alpha was used as immunogen.
Alternative Name IKK alpha
Background IKK-alpha (I-Kappa-B kinase-alpha) is a member of the IKK complex which is composed of IKK-alpha, IKK-beta, IKK-gamma and IKAP (1-2). Phosphorylation of I-Kappa-B on a serine residue by the IKK complex frees NF-kB from I-Kappa-B and marks it for degradation via ubiquination. Activation of NFKB by TNF requires phosphorylation of IKK-alpha at threonine 23 and serine 176 by AKT1 and NIK respectively (3). It has been demonstrated that IKK-alpha can accumulate in the nucleus after cytokine exposure (4). In the nucleus IKK-alpha interacts with CREB-binding protein in conjunction with RELA to be recruited to the NF-kappa-B-responsive promoters in order to mediate the cytokine-induced phosphorylation and subsequent acetylation of specific residues in histone H3 (5).
Synonyms: CHUK, IKKA, TCF16, Inhibitor of nuclear factor kappa-B kinase subunit alpha, Conserved helix-loop-helix ubiquitous kinase,Nuclear factor NF-kappa-B inhibitor kinase alpha
Molecular Weight 85 kDA
Gene ID 1147
UniProt O15111
Research Area Signaling, Phospho-specific antibodies, Cell Signaling, Protein Modifications, Chromatin, Cell Structure
Application Notes The suggested dilution is: WB: = 1:1000 , IHC: = 1:50
Comment

Background: IKK-alpha (I-Kappa-B kinase-alpha) is a member of the IKK complex which is composed of IKK-alpha, IKK-beta, IKK-gamma and IKAP (1-2). Phosphorylation of I-Kappa-B on a serine residue by the IKK complex frees NF-kB from I-Kappa-B and marks it for degradation via ubiquination. Activation of NFKB by TNF requires phosphorylation of IKK-alpha at threonine 23 and serine 176 by AKT1 and NIK respectively (3). It has been demonstrated that IKK-alpha can accumulate in the nucleus after cytokine exposure (4). In the nucleus IKK-alpha interacts with CREB-binding protein in conjunction with RELA to be recruited to the NF-kappa-B-responsive promoters in order to mediate the cytokine-induced phosphorylation and subsequent acetylation of specific residues in histone H3 (5).

Restrictions For Research Use only
Format Liquid
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment IKK alpha Antibody (N-term) can be stored at -20°C for up to 12 months from time of receipt.
Expiry Date 12 months
Background publications Zandi, Rothwarf, Delhase et al.: "The IkappaB kinase complex (IKK) contains two kinase subunits, IKKalpha and IKKbeta, necessary for IkappaB phosphorylation and NF-kappaB activation." in: Cell, Vol. 91, Issue 2, pp. 243-52, 1997 (PubMed).

Scheidereit: "Signal transduction. Docking IkappaB kinases." in: Nature, Vol. 395, Issue 6699, pp. 225-6, 1998 (PubMed).

Ozes, Mayo, Gustin et al.: "NF-kappaB activation by tumour necrosis factor requires the Akt serine-threonine kinase." in: Nature, Vol. 401, Issue 6748, pp. 82-5, 1999 (PubMed).

Yamamoto, Verma, Prajapati et al.: "Histone H3 phosphorylation by IKK-alpha is critical for cytokine-induced gene expression." in: Nature, Vol. 423, Issue 6940, pp. 655-9, 2003 (PubMed).

Anest, Hanson, Cogswell et al.: "A nucleosomal function for IkappaB kinase-alpha in NF-kappaB-dependent gene expression." in: Nature, Vol. 423, Issue 6940, pp. 659-63, 2003 (PubMed).

Validation Images
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