Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase beta (IKBKB) antibody

Details for Product No. ABIN649912
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Antigen
Synonyms
ikk-beta, ikk2, ikkb, ikkbeta, IKBKB, IKKB, CG4201, DIK, DLAK, DmIKK, DmIKKBeta, DmIKKb, DmIKKbeta, DmIkkbeta, Dmel\\CG4201, Dmikkb, Dmikkbeta, IK, IKK, IKK-beta, IKKb, IKKbeta, IRD-5, IRD5, Ik, Ikkb, ... show more
ikk-beta, ikk2, ikkb, ikkbeta, IKBKB, IKKB, CG4201, DIK, DLAK, DmIKK, DmIKKBeta, DmIKKb, DmIKKbeta, DmIkkbeta, Dmel\\CG4201, Dmikkb, Dmikkbeta, IK, IKK, IKK-beta, IKKb, IKKbeta, IRD-5, IRD5, Ik, Ikkb, Ikkbeta, Ird-5, Ird5, LRK, Lak, anon-89Bd, dIKK, dIKK-beta, dIKKbeta, dLak, dmIKKbeta, ikk, ird, ird5/DmIkk[beta], IKK2, NFKBIKB, AIM-1, AI132552, IKK-2, IKK[b] show less
Reactivity
Human
(417), (152), (140), (98), (98), (86), (49), (1)
Host
Rabbit
(375), (49), (5), (1)
Clonality
Monoclonal
Conjugate
Un-conjugated
(26), (26), (26), (16), (16), (16), (10), (10), (10), (10), (10), (10), (10), (10)
Application
Western Blotting (WB), Immunohistochemistry (IHC)
(178), (168), (125), (100), (59), (49), (30), (18), (18), (4), (3), (3), (1)
Pubmed 5 references available
Quantity 100 µL
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Catalog No. ABIN649912
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Specificity A synthetic peptide corresponding to residues near the C-term of human IKK-beta was used as immunogen.
Alternative Name IKK beta
Background IKK-beta (I-Kappa-B kinase-beta) is a member of the IKK complex which is composed of IKK-alpha, IKK-beta, IKK-gamma and IKAP (1-2). Phosphorylation of I-Kappa-B on a serine residue by the IKK complex frees NF-kB from I-Kappa-B and marks it for degradation via ubiquination. IKK-beta has been shown to activate NF-kB and phosphorylate IKB-alpha and beta (3). Phosphorylation of 2 sites at the activation loop of IKK-beta is essential for activation of IKK by TNF and IL1. Once activated, IKK-beta autophosphorylates which in turn decreases IKK activity and prevents prolonged activation of the inflammatory response (4). Additionally, IKK-beta activity can also be regulated by MEKK-1 (5).
Synonyms: CHUK, IKKA, TCF16, Inhibitor of nuclear factor kappa-B kinase subunit alpha, Conserved helix-loop-helix ubiquitous kinase,Nuclear factor NF-kappa-B inhibitor kinase alpha
Molecular Weight 85 kDA
Gene ID 1147
UniProt O15111
Research Area Signaling, Phospho-specific antibodies, Cell Signaling, Protein Modifications, Chromatin, Cell Structure
Application Notes IHC: = 1:50-100, WB: = 1:1000-5000
Comment

Background: IKK-beta (I-Kappa-B kinase-beta) is a member of the IKK complex which is composed of IKK-alpha, IKK-beta, IKK-gamma and IKAP (1-2). Phosphorylation of I-Kappa-B on a serine residue by the IKK complex frees NF-kB from I-Kappa-B and marks it for degradation via ubiquination. IKK-beta has been shown to activate NF-kB and phosphorylate IKB-alpha and beta (3). Phosphorylation of 2 sites at the activation loop of IKK-beta is essential for activation of IKK by TNF and IL1. Once activated, IKK-beta autophosphorylates which in turn decreases IKK activity and prevents prolonged activation of the inflammatory response (4). Additionally, IKK-beta activity can also be regulated by MEKK-1 (5).

Restrictions For Research Use only
Format Liquid
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment IKK beta Antibody can be stored at -20°C for up to 12 months from time of receipt.
Expiry Date 12 months
Background publications Zandi, Rothwarf, Delhase et al.: "The IkappaB kinase complex (IKK) contains two kinase subunits, IKKalpha and IKKbeta, necessary for IkappaB phosphorylation and NF-kappaB activation." in: Cell, Vol. 91, Issue 2, pp. 243-52, 1997 (PubMed).

Woronicz, Gao, Cao et al.: "IkappaB kinase-beta: NF-kappaB activation and complex formation with IkappaB kinase-alpha and NIK." in: Science (New York, N.Y.), Vol. 278, Issue 5339, pp. 866-9, 1997 (PubMed).

Nakano, Shindo, Sakon et al.: "Differential regulation of IkappaB kinase alpha and beta by two upstream kinases, NF-kappaB-inducing kinase and mitogen-activated protein kinase/ERK kinase kinase-1." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 7, pp. 3537-42, 1998 (PubMed).

Scheidereit: "Signal transduction. Docking IkappaB kinases." in: Nature, Vol. 395, Issue 6699, pp. 225-6, 1998 (PubMed).

Delhase, Hayakawa, Chen et al.: "Positive and negative regulation of IkappaB kinase activity through IKKbeta subunit phosphorylation." in: Science (New York, N.Y.), Vol. 284, Issue 5412, pp. 309-13, 1999 (PubMed).

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