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Integrin-Linked Kinase (ILK) antibody

Antigen

Integrin-Linked Kinase (ILK)
Synonyms ESTM24, AA511515, P59, DKFZp686F1765, CT29478, DmILK, ILK, l(3)78Ca, DmelCG10504, CG10504, MGC65842, zgc:65842, ilk, MGC82501, MGC130696, MGC129022, Ilk, p59
Clonality Monoclonal
Host
Alternatives

Rabbit
Reactivity
Alternatives

Human, Rat (Rattus)
Conjugate
Alternatives Un-conjugated
Application
Alternatives Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)
2 references available
Certificates ISO 9001:2008
Catalog no. ABIN649928
Quantity 100ul  (Variants)
Price 450.00 $   Plus shipping costs $45.00
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Additional Information
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Alternative name ILK
Gene ID 3611
UniProt Q13418
Description Other names: ILK, ILK1, ILK2, Integrin-linked protein kinase, ILK-1,ILK-2,59 kDa serine/threonine-protein kinase,p59ILK
Characteristics Rabbit Monoclonal Antibody
Specificity A synthetic peptide corresponding to residues near N-term of human ILK protein was used as immunogen
Molecular Weight 51 kDA
Comments

Background: Integrin-linked kinase (ILK) is a key cytoplasmic plaque protein involved in the mediation of integrin functions. It plays a key role as a regulator during cell survival, cell proliferation, cell migration, and cell to cell adhesion (1). ILK links integrins to the actin cytoskeleton, allowing transduction of signals through integrins to the extracellular matrix (ECM). Also, ILK has been linked to the regulation of smooth contraction and cell motility in non-muscle cells through phosphorylation of myosin (2). Irregular activity level of ILK has been linked to expression of certain tumor. ILK overexpression caused by inactivating mutation in tumor suppressors (e.g. PTEN and APC) lead to tumor formation in transgenic mouse. Up-regulated ILK expression and activity are seen in Ewings scaromas, colorectal cancers, and late staged prostate cancers (3).

Application Details
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Application Notes The suggested dilution is: WB: ~~ 1:10000~200000 IP: ~~ 1:30 ICC: ~~ 1:100~250 IHC: ~~ 1:50~100
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Storage ILK Antibody can be stored at -20°C for up to 12 months from time of receipt.
Research Area Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Structure, Signaling, Chromatin
Restrictions For Research Use only

Publications
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Product Marotta, Tan, Gray et al.: "Dysregulation of integrin-linked kinase (ILK) signaling in colonic polyposis." in: Oncogene, Vol. 20, Issue 43, pp. 6250-7, 2001 (PubMed).

Wu, Dedhar: "Integrin-linked kinase (ILK) and its interactors: a new paradigm for the coupling of extracellular matrix to actin cytoskeleton and signaling complexes." in: The Journal of cell biology, Vol. 155, Issue 4, pp. 505-10, 2001 (PubMed).

Alternatives
Application Details Publications Variants back to top

Alternatives for antigen "Integrin-Linked Kinase (ILK)", type "Antibodies"
Hosts Rabbit (72), Goat (1), Mouse (1)
Reactivities Human (71), Rat (Rattus) (38), Mouse (Murine) (37), Chicken (34), Cow (Bovine) (34), Dog (Canine) (34), Horse (Equine) (15), Pig (Porcine) (15), Sheep (Ovine) (15), Cat (Feline) (1), Monkey (1)
Applications ELISA (34), Western Blotting (WB) (34), Immunofluorescence (IF) (31), Immunohistochemistry (IHC) (14), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (12), Dot Blot (Dot) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Flow Cytometry (FACS) (2), Immunoprecipitation (IP) (2), Immunocytochemistry (ICC) (1), Immunoelectron Microscopy (IEM) (1)
Conjugates Alexa Fluor 350 (4), Alexa Fluor 488 (4), Alexa Fluor 555 (4), Alexa Fluor 647 (4), PE,Cy5.5 (4), Biotin (2), FITC (2), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy7 (1)
Epitopes pSer246 (8), pThr173 (7), C-Term (5), N-Term (4), pSer259 (4), Ser246 (1), Ser259 (1), Ser343 (1), Thr173 (1), Thr197 (1), pSer343 (1), pSer476 (1)