Integrin-Linked Kinase (ILK) antibody

Antigen: Integrin-Linked Kinase (ILK)

Synonyms: ESTM24, AA511515, P59, DKFZp686F1765, CT29478, DmILK, ILK, l(3)78Ca, DmelCG10504, CG10504, MGC65842, zgc:65842, ilk, MGC82501, MGC130696, MGC129022, Ilk, p59

Clonality: Monoclonal

Host: Rabbit

Reactivity: Human, Rat (Rattus)

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Certificates: ISO 9001:2008

Catalog no.: ABIN649929

Additional Information

Alternative name: ILK

Gene ID: 3611

UniProt: Q13418

Description: Other names: ILK, ILK1, ILK2, Integrin-linked protein kinase, ILK-1,ILK-2,59 kDa serine/threonine-protein kinase,p59ILK

Characteristics: Rabbit Monoclonal Antibody

Specificity: A synthetic peptide corresponding to residues in human ILK was used as an immunogen.

Molecular Weight: 51 kDA

Comments:

Background: Integrin-linked kinase (ILK) is a key cytoplasmic plaque protein involved in the mediation of integrin functions. It plays a key role as a regulator during cell survival, cell proliferation, cell migration, and cell to cell adhesion (1). ILK links integrins to the actin cytoskeleton, allowing transduction of signals through integrins to the extracellular matrix. Also, ILK has been linked to the regulation of smooth contraction and cell motility in non-muscle cells through phosphorylation of myosin (2). Irregular activity level of ILK has been linked to expression of certain tumor. ILK overexpression caused by inactivating mutation in tumor suppressors (e.g. PTEN and APC) lead to tumor formation in transgenic mouse. Up-regulated ILK expression and activity are seen in Ewings scaromas, colorectal cancers, and late staged prostate cancers (3).

Application Details

Application Notes: The suggested dilution is: WB: ~~ 1:5000~10000 FC: ~~ 1:60 ICC: ~~ 1:100~250

Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage: ILK Antibody can be stored at -20°C for up to 12 months from time of receipt.

Research Area: Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Structure, Signaling, Chromatin

Restrictions: For Research Use only

Publications

Product: Marotta, Tan, Gray et al.: "Dysregulation of integrin-linked kinase (ILK) signaling in colonic polyposis." in: Oncogene, Vol. 20, Issue 43, pp. 6250-7, 2001 (PubMed).

Wu, Dedhar: "Integrin-linked kinase (ILK) and its interactors: a new paradigm for the coupling of extracellular matrix to actin cytoskeleton and signaling complexes." in: The Journal of cell biology, Vol. 155, Issue 4, pp. 505-10, 2001 (PubMed).