Indian Hedgehog (IHH) antibody

Antigen: Indian Hedgehog (IHH)

Synonyms: BDA1, HHG2, ihh, X-hh, bhh-a, IHH

Clonality: Monoclonal

Host: Rabbit

Reactivity: Human, Rat (Rattus)

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Certificates: ISO 9001:2008

Catalog no.: ABIN649930

Additional Information

Gene ID: 3549

UniProt: Q14623

Description: Other names: IHH, Indian hedgehog protein C-product, HHG-2

Characteristics: Rabbit Monoclonal Antibody

Specificity: A synthetic peptide corresponding to residues near the N-terminus of human IHH was used as an immunogen.

Molecular Weight: 45 kDA

Comments:

Background: Hedgehog, WNT, FGF and BMP signaling pathways network together during embryogenesis, tissue regeneration, and carcinogenesis. Aberrant activation of Hedgehog signaling pathway leads to pathological consequences in a variety of human tumors, such as gastric cancer and pancreatic cancer (1). The hedgehog family of morphogens are regulators of cell proliferation, differentiation and cell-cell communication. These morphogens have been shown to have important roles in organogenesis, spermatogenesis, stem cell maintenance and oncogenesis. Indian hedgehog (IHH) has been shown to be expressed in the uterine epithelium under the control of the steroid hormone, progesterone (2). It has been shown that IHH, synthesized by prehypertrophic and hypertrophic chondrocytes, regulates the site of hypertrophic differentiation by signaling to the periarticular growth plate and also determines the site of bone collar formation in the adjacent perichondrium. By providing crucial local signals from prehypertrophic and hypertrophic chondrocytes to both chondrocytes and preosteoblasts, IHH couples chondrogenesis to osteogenesis in endochondral bone development (3).

Application Details

Application Notes: The suggested dilution is: WB: ~~ 1:1000 IP: ~~ 1:30 IHC: ~~ 1:250~500

Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage: Indian Hedgehog Antibody (IHH) can be stored at -20°C for up to 12 months from time of receipt.

Research Area: Cell Signaling, Protein Modifications, Cell Structure, Metabolism

Restrictions: For Research Use only

Publications

Product: Chung, Schipani, McMahon et al.: "Indian hedgehog couples chondrogenesis to osteogenesis in endochondral bone development." in: The Journal of clinical investigation, Vol. 107, Issue 3, pp. 295-304, 2001 (PubMed).