Interleukin-1 Receptor-Associated Kinase 3 (IRAK3) (C-Term) antibody

Antigen: Interleukin-1 Receptor-Associated Kinase 3 (IRAK3)

Synonyms: ASRT5, IRAKM, IRAK-M, FLJ13601, IRAK3, IrakM, AI563835, 4833428C18Rik, si:dkey-150i15.2

Binding Site: C-Term

Clonality: Monoclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunoprecipitation (IP)

Certificates: ISO 9001:2008

Catalog no.: ABIN649941

Additional Information

Alternative name: IRAK-3

Gene ID: 11213

UniProt: Q9Y616

Description: Other names: IRAK3, Interleukin-1 receptor-associated kinase 3, IL-1 receptor-associated kinase M

Characteristics: Rabbit Monoclonal Antibody

Specificity: A synthetic peptide corresponding to residues in the C-term of human IRAK-3 was used as immunogen.

Molecular Weight: 68 kDA

Comments:

Background: IRAK-3 (also known as IRAK-M) is a member of the interleukine-1 receptor-associated kinase (IRAK) family. The IRAK family is implicated in the Toll-like receptor (TLR) and Il-1R signaling pathway (1). Upon stimulation, IRAK gets phosphorylated and activates NF-kB (2). Unlike IRAK, IRAK2 and IRAK4 which are expressed ubiquitously, IRAK-3 is mostly expressed in monocytes and macrophages (3). IRAK-M and IRAK-2 lack kinase activity, while IRAK-3 has a weak capacity to be phosphorylated. Additionally, IRAK-3 has been found to negatively regulate TRL signaling to protect from endotoxic shock (5).

Application Details

Application Notes: The suggested dilution is: WB: ~~ 1:1000 IP: ~~ 1:50

Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage: IRAK-3 Antibody (C-term) can be stored at -20°C for up to 12 months from time of receipt.

Research Area: Phospho-specific antibodies, Cell Signaling, Protein Modifications, Signaling, Chromatin, Cell Structure

Restrictions: For Research Use only

Publications

Product: Cao, Henzel, Gao: "IRAK: a kinase associated with the interleukin-1 receptor." in: Science (New York, N.Y.), Vol. 271, Issue 5252, pp. 1128-31, 1996 (PubMed).

Kanakaraj, Ngo, Wu et al.: "Defective interleukin (IL)-18-mediated natural killer and T helper cell type 1 responses in IL-1 receptor-associated kinase (IRAK)-deficient mice." in: The Journal of experimental medicine, Vol. 189, Issue 7, pp. 1129-38, 1999 (PubMed).