Interferon Regulatory Factor 3 (IRF3) (C-Term) antibody

Antigen: Interferon Regulatory Factor 3 (IRF3)

Synonyms: IRF-3, MGC91046, C920001K05Rik, MGC94729, IRF3, irf3

Binding Site: C-Term

Clonality: Monoclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunoprecipitation (IP)

Certificates: ISO 9001:2008

Catalog no.: ABIN649943

Additional Information

Alternative name: IRF-3

Gene ID: 3661

UniProt: Q14653

Description: Other names: IRF3, Interferon regulatory factor 3

Characteristics: Rabbit Monoclonal Antibody

Specificity: A synthetic peptide corresponding to residues on the C-terminus of human IRF-3 was used as an immunogen.

Molecular Weight: 51 kDA

Comments:

Background: A family of interferon (IFN) regulatory factors (IRFs) has been shown to play a role in the transcription of IFN genes as well as IFN-stimulated genes. The IRF-3 gene encodes a 50-kDa protein that binds specifically to the IFN-stimulated response element (ISRE) but not to the IRF-1 binding site PRD-I. Overexpression of IRF-3 stimulates expression of the IFN-stimulated gene 15 (ISG15) promoter, an ISRE-containing promoter. The high amino acid homology between IRF-3 and ISG factor 3 gamma polypeptide (ISGF3 gamma) and their similar binding properties indicate that, like ISGF3 gamma, IRF-3 may activate transcription by complex formation with other transcriptional factors, possibly members of the Stat family (1). IRF-3 is expressed constitutively in a variety of tissues, and the relative levels of IRF-3 mRNA do not change in virus-infected or IFN-treated cells. (2). In uninfected cells, the IRF-3 component of DRAF1 resides in the cytoplasm. The cytoplasmic localization of IRF-3 is dependent on a nuclear export signal, and IRF-3 is recognized by the chromosome region maintenance 1 (CRM1) (also known as exportin 1) shuttling receptor. Following infection and specific phosphorylation, IRF-3 accumulates in the nucleus where it associates with CBP and p300 (3).

Application Details

Application Notes: The suggested dilution is: WB: ~~ 1:1000~2000 IP: ~~ 1:20 FC: ~~ 1:10 IHC: ~~ 1:100~250

Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage: IRF-3 Antibody (C-term) can be stored at -20°C for up to 12 months from time of receipt.

Research Area: Translation Factors, Phospho-specific antibodies, Protein Modifications, Transcription Factors, Signaling, Cell Structure

Restrictions: For Research Use only

Publications

Product: Au, Moore, Lowther et al.: "Identification of a member of the interferon regulatory factor family that binds to the interferon-stimulated response element and activates expression of interferon-induced genes." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 92, Issue 25, pp. 11657-61, 1996 (PubMed).

Lin, Heylbroeck, Pitha et al.: "Virus-dependent phosphorylation of the IRF-3 transcription factor regulates nuclear translocation, transactivation potential, and proteasome-mediated degradation." in: Molecular and cellular biology, Vol. 18, Issue 5, pp. 2986-96, 1998 (PubMed).