|+1 404 474 4654|
|+1 888 205 9894 (TF)|
Interferon Regulatory Factor 3 (IRF3) (N-Term) antibody
|Synonyms||IRF-3, MGC91046, C920001K05Rik, MGC94729, IRF3, irf3|
Alternatives Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)
|2 references available|
|Price||450.00 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
|Description||Other names: IRF3, Interferon regulatory factor 3|
|Characteristics||Rabbit Monoclonal Antibody|
|Specificity||A synthetic peptide corresponding to residues surrounding the N-terminus of human IRF-3 was used as an immunogen.|
|Molecular Weight||51 kDA|
Background: A family of interferon (IFN) regulatory factors (IRFs) have been shown to play a role in transcription of IFN genes as well as IFN-stimulated genes. The IRF-3 gene encodes a 50-kDa protein that binds specifically to the IFN-stimulated response element (ISRE) but not to the IRF-1 binding site PRD-I. Overexpression of IRF-3 stimulates expression of the IFN-stimulated gene 15 (ISG15) promoter, an ISRE-containing promoter. The high amino acid homology between IRF-3 and ISG factor 3 gamma polypeptide (ISGF3 gamma) and their similar binding properties indicate that, like ISGF3 gamma, IRF-3 may activate transcription by complex formation with other transcriptional factors, possibly members of the Stat family (1). IRF-3 is expressed constitutively in a variety of tissues, and the relative levels of IRF-3 mRNA do not change in virus-infected or IFN-treated cells (2). In uninfected cells, the IRF-3 component of DRAF1 resides in the cytoplasm. The cytoplasmic localization of IRF-3 is dependent on a nuclear export signal, and IRF-3 is recognized by the chromosome region maintenance 1 (CRM1) (also known as exportin 1) shuttling receptor. Following infection and specific phosphorylation, IRF-3 accumulates in the nucleus where it associates with CBP and p300 (3).
|Application Notes||The suggested dilution is: WB: ~~ 1:2000~10000 FC: ~~ 1:20 IHC: ~~ 1:100~250|
|Buffer||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||IRF-3 Antibody (N-term) can be stored at -20°C for up to 12 months from time of receipt.|
|Research Area||Translation Factors, Phospho-specific antibodies, Protein Modifications, Transcription Factors, Signaling, Cell Structure|
|Restrictions||For Research Use only|
Au, Moore, Lowther et al.: "Identification of a member of the interferon regulatory factor family that binds to the interferon-stimulated response element and activates expression of interferon-induced genes." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 92, Issue 25, pp. 11657-61, 1996 (PubMed).
Lin, Heylbroeck, Pitha et al.: "Virus-dependent phosphorylation of the IRF-3 transcription factor regulates nuclear translocation, transactivation potential, and proteasome-mediated degradation." in: Molecular and cellular biology, Vol. 18, Issue 5, pp. 2986-96, 1998 (PubMed).