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Interferon Regulatory Factor 3 (IRF3) (pSer386) antibody

Details for Product No. ABIN649945
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Antigen
Synonyms C920001K05Rik, IRF-3, IRF3, irf3
Epitope
pSer386
(28), (25), (21), (15), (9), (9), (7), (4), (4), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(200), (80), (54), (38), (37), (37), (4)
Host
Rabbit
(180), (33), (1)
Clonality
Monoclonal
Conjugate
Un-conjugated
(4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (1), (1), (1)
Application
Western Blotting (WB), Immunofluorescence (IF)
(175), (103), (57), (36), (34), (30), (30), (17), (6), (5), (4), (4)
Pubmed 2 references available
Catalog no. ABIN649945
Quantity 100 µL
Price
507.14 $   Plus shipping costs $45.00
Options
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Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Specificity A phospho-specific peptide corresponding to residues surrounding Serine 386 of human IRF3 was used as an immunogen. The antibody only detects IRF3 phosphorylated on Serine 386.
Alternative Name IRF3
Background A family of interferon (IFN) regulatory factors (IRFs) have been shown to play a role in transcription of IFN genes as well as IFN-stimulated genes. The IRF-3 gene encodes a 50-kDa protein that binds specifically to the IFN-stimulated response element (ISRE) (1). IRF-3 is activated through phosphorylation by the kinases IKK epsilon and/or TBK1. Phosphorylation results in IRF-3 dimerization and removal of an autoinhibitory structure to allow interaction with the coactivators CBP/p300. Phosphorylation at Serine 396 through Serine 405 alleviates autoinhibition to allow interaction with CREB-binding protein and facilitates phosphorylation at Serine 385 or Serine 386 (2). Serine 386 is suggested to be the target of the IRF-3 kinase and critical determinant for the activation of IRF-3 (3).
Synonyms: IRF3, Interferon regulatory factor 3
Molecular Weight 51 kDA
Gene ID 3661
UniProt Q14653
Research Area Translation Factors, Phospho-specific antibodies, Protein Modifications, Transcription Factors, Signaling, Cell Structure
Application Notes The suggested dilution is: WB: = 1:2000-10000
Comment

Background: A family of interferon (IFN) regulatory factors (IRFs) have been shown to play a role in transcription of IFN genes as well as IFN-stimulated genes. The IRF-3 gene encodes a 50-kDa protein that binds specifically to the IFN-stimulated response element (ISRE) (1). IRF-3 is activated through phosphorylation by the kinases IKK epsilon and/or TBK1. Phosphorylation results in IRF-3 dimerization and removal of an autoinhibitory structure to allow interaction with the coactivators CBP/p300. Phosphorylation at Serine 396 through Serine 405 alleviates autoinhibition to allow interaction with CREB-binding protein and facilitates phosphorylation at Serine 385 or Serine 386 (2). Serine 386 is suggested to be the target of the IRF-3 kinase and critical determinant for the activation of IRF-3 (3).

Restrictions For Research Use only
Format Liquid
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment IRF3 Antibody Phospho (pS386) can be stored at -20°C for up to 12 months from time of receipt.
Expiry Date 12 months
Background publications Au, Moore, Lowther et al.: "Identification of a member of the interferon regulatory factor family that binds to the interferon-stimulated response element and activates expression of interferon-induced genes." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 92, Issue 25, pp. 11657-61, 1996 (PubMed).

Panne, McWhirter, Maniatis et al.: "Interferon regulatory factor 3 is regulated by a dual phosphorylation-dependent switch." in: The Journal of biological chemistry, Vol. 282, Issue 31, pp. 22816-22, 2007 (PubMed).

Hosts (180), (33), (1)
Reactivities (200), (80), (54), (38), (37), (37), (4)
Applications (175), (103), (57), (36), (34), (30), (30), (17), (6), (5), (4), (4)
Conjugates (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (1), (1), (1)
Epitopes (28), (25), (21), (15), (9), (9), (7), (4), (4), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
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