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Interferon Regulatory Factor 3 (IRF3) (pSer386) antibody

Antigen

Interferon Regulatory Factor 3 (IRF3)
Synonyms IRF-3, MGC91046, C920001K05Rik, MGC94729, IRF3, irf3
Binding Site
Alternatives

pSer386
Clonality Monoclonal
Host
Alternatives

Rabbit
Reactivity
Alternatives

Human
Conjugate
Alternatives Un-conjugated
Application
Alternatives Western Blotting (WB), Immunocytochemistry (ICC)
2 references available
Certificates ISO 9001:2008
Catalog no. ABIN649945
Quantity 100ul
Price 507.14 $   Plus shipping costs $45.00
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Additional Information
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Alternative name IRF3
Gene ID 3661
UniProt Q14653
Description Other names: IRF3, Interferon regulatory factor 3
Characteristics Rabbit Monoclonal Antibody
Specificity A phospho-specific peptide corresponding to residues surrounding Serine 386 of human IRF3 was used as an immunogen. The antibody only detects IRF3 phosphorylated on Serine 386.
Molecular Weight 51 kDA
Comments

Background: A family of interferon (IFN) regulatory factors (IRFs) have been shown to play a role in transcription of IFN genes as well as IFN-stimulated genes. The IRF-3 gene encodes a 50-kDa protein that binds specifically to the IFN-stimulated response element (ISRE) (1). IRF-3 is activated through phosphorylation by the kinases IKK epsilon and/or TBK1. Phosphorylation results in IRF-3 dimerization and removal of an autoinhibitory structure to allow interaction with the coactivators CBP/p300. Phosphorylation at Serine 396 through Serine 405 alleviates autoinhibition to allow interaction with CREB-binding protein and facilitates phosphorylation at Serine 385 or Serine 386 (2). Serine 386 is suggested to be the target of the IRF-3 kinase and critical determinant for the activation of IRF-3 (3).

Application Details
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Application Notes The suggested dilution is: WB: ~~ 1:2000~10000 ICC: ~~ 1:250~500
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Storage IRF3 Antibody Phospho (pS386) can be stored at -20°C for up to 12 months from time of receipt.
Research Area Translation Factors, Phospho-specific antibodies, Protein Modifications, Transcription Factors, Signaling, Cell Structure
Restrictions For Research Use only

Publications
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Product Au, Moore, Lowther et al.: "Identification of a member of the interferon regulatory factor family that binds to the interferon-stimulated response element and activates expression of interferon-induced genes." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 92, Issue 25, pp. 11657-61, 1996 (PubMed).

Panne, McWhirter, Maniatis et al.: "Interferon regulatory factor 3 is regulated by a dual phosphorylation-dependent switch." in: The Journal of biological chemistry, Vol. 282, Issue 31, pp. 22816-22, 2007 (PubMed).

Alternatives
Application Details Publications back to top

Alternatives for antigen "Interferon Regulatory Factor 3 (IRF3)", type "Antibodies"
Hosts Rabbit (99), Mouse (19), Goat (1)
Reactivities Human (120), Mouse (Murine) (57), Rat (Rattus) (53), Cow (Bovine) (38), Dog (Canine) (38), Pig (Porcine) (38), Horse (Equine) (18), Rabbit (18), Monkey (2), Cat (Feline) (1), Chicken (1)
Applications Western Blotting (WB) (82), ELISA (48), Immunofluorescence (IF) (48), Immunohistochemistry (IHC) (23), Flow Cytometry (FACS) (21), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (19), Immunoprecipitation (IP) (9), Immunocytochemistry (ICC) (8), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (6), Immunoelectron Microscopy (IEM) (2), Dot Blot (Dot) (1), Immunoassay (IA) (1)
Conjugates Alexa Fluor 350 (3), Alexa Fluor 488 (3), Alexa Fluor 555 (3), Alexa Fluor 647 (3), Biotin (2), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), FITC (2), Gold (2), HRP (2), PE (2), PE,Cy5.5 (2), PE,Cy3 (1), PE,Cy5 (1), PE,Cy7 (1), PE-Cy3 (1), PE-Cy5 (1), PE-Cy5.5 (1), PE-Cy7 (1), Un-conjugated (1)
Epitopes pSer386 (19), C-Term (8), pSer396 (6), AA 108-166 (4), Transcript Variant 1 (3), pSer385,C-Term (3), pSer386,C-Term (3), pSer396,C-Term (3), Internal Region (2), AA 100-150 (1), AA 206-427 (1), AA 388-402 (1), Middle Region (1), N-Term (1), Pro173 (1), pSer385 (1), pSer398 (1), pThr387 (1)