|+1 404 474 4654|
|+1 888 205 9894 (TF)|
Interferon Regulatory Factor 3 (IRF3) (pSer386) antibody
|Synonyms||IRF-3, MGC91046, C920001K05Rik, MGC94729, IRF3, irf3|
Alternatives Western Blotting (WB), Immunocytochemistry (ICC)
|2 references available|
|Price||507.14 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
|Description||Other names: IRF3, Interferon regulatory factor 3|
|Characteristics||Rabbit Monoclonal Antibody|
|Specificity||A phospho-specific peptide corresponding to residues surrounding Serine 386 of human IRF3 was used as an immunogen. The antibody only detects IRF3 phosphorylated on Serine 386.|
|Molecular Weight||51 kDA|
Background: A family of interferon (IFN) regulatory factors (IRFs) have been shown to play a role in transcription of IFN genes as well as IFN-stimulated genes. The IRF-3 gene encodes a 50-kDa protein that binds specifically to the IFN-stimulated response element (ISRE) (1). IRF-3 is activated through phosphorylation by the kinases IKK epsilon and/or TBK1. Phosphorylation results in IRF-3 dimerization and removal of an autoinhibitory structure to allow interaction with the coactivators CBP/p300. Phosphorylation at Serine 396 through Serine 405 alleviates autoinhibition to allow interaction with CREB-binding protein and facilitates phosphorylation at Serine 385 or Serine 386 (2). Serine 386 is suggested to be the target of the IRF-3 kinase and critical determinant for the activation of IRF-3 (3).
|Application Notes||The suggested dilution is: WB: ~~ 1:2000~10000 ICC: ~~ 1:250~500|
|Buffer||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||IRF3 Antibody Phospho (pS386) can be stored at -20°C for up to 12 months from time of receipt.|
|Research Area||Translation Factors, Phospho-specific antibodies, Protein Modifications, Transcription Factors, Signaling, Cell Structure|
|Restrictions||For Research Use only|
Au, Moore, Lowther et al.: "Identification of a member of the interferon regulatory factor family that binds to the interferon-stimulated response element and activates expression of interferon-induced genes." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 92, Issue 25, pp. 11657-61, 1996 (PubMed).
Panne, McWhirter, Maniatis et al.: "Interferon regulatory factor 3 is regulated by a dual phosphorylation-dependent switch." in: The Journal of biological chemistry, Vol. 282, Issue 31, pp. 22816-22, 2007 (PubMed).