|+1 404 474 4654|
|+1 888 205 9894 (TF)|
Janus Kinase 2 (JAK2) (pTyr1007, pTyr1008) antibody
|Synonyms||JTK10, Fd17, C81284, AI504024, JAK2, DKFZp469G074|
Human, Rat (Rattus)
Alternatives Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)
|3 references available|
|Price||507.14 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
|Description||Other names: JAK2, Tyrosine-protein kinase JAK2, Janus kinase 2|
|Characteristics||Rabbit Monoclonal Antibody|
|Specificity||A synthetic phospho-peptide corresponding to residues surrounding Tyr1007 and Tyr1008 of human JAK2 was used as immunogen. The antibody only detects JAK2 phosphorylated on Tyrosine 1007 and 1008.|
|Molecular Weight||130 kDA|
Background: Jak2 is a member of the Janus family tyrosine kinases (Jaks), whose function is to transduce extracellular signals from cytokines and interferons. Jaks associate with cytokine receptors, upon ligand binding, Jaks phosphorylate the receptor. The phosphorylated receptors are bound by SH2-containing proteins, one class of which is the signal transducers and activators of transcription (STATs). Activated STATs, then, translocate to the nucleus to mediate gene transcription (1,2). Tyr1007/1008 of Jak2 are the homologous Tyr1054/1055 in Tyk2, and phosphorylation at these residues is needed for Tyk2 activation (3). A chromosomal aberration involving Jak2 is found in a form of pre-B acute myeloid leukemia (4).
|Application Notes||The suggested dilution is: WB: ~~ 1:5000~200000 IP: ~~ 1:50 ICC: ~~ 1:50~100 IHC: ~~ 1:50~100|
|Buffer||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||JAK2 Antibody Phospho (pY1007/1008) can be stored at -20°C for up to 12 months from time of receipt.|
|Research Area||Ubiquitin-related antibodies, Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Structure, Cancer, Signaling, Chromatin|
|Restrictions||For Research Use only|
Gauzzi, Velazquez, McKendry et al.: "Interferon-alpha-dependent activation of Tyk2 requires phosphorylation of positive regulatory tyrosines by another kinase." in: The Journal of biological chemistry, Vol. 271, Issue 34, pp. 20494-500, 1996 (PubMed).
Peeters, Raynaud, Cools et al.: "Fusion of TEL, the ETS-variant gene 6 (ETV6), to the receptor-associated kinase JAK2 as a result of t(9;12) in a lymphoid and t(9;15;12) in a myeloid leukemia." in: Blood, Vol. 90, Issue 7, pp. 2535-40, 1997 (PubMed).
OShea, Notarangelo, Johnston et al.: "Advances in the understanding of cytokine signal transduction: the role of Jaks and STATs in immunoregulation and the pathogenesis of immunodeficiency." in: Journal of clinical immunology, Vol. 17, Issue 6, pp. 431-47, 1998 (PubMed).