Janus Kinase 2 (JAK2) (pTyr1007), (pTyr1008) antibody

Details for Product No. ABIN649956
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Antigen
Synonyms JAK2bCA, jak2, JTK10, THCYT3, AI504024, C81284, Fd17, JAK-2
Epitope
pTyr1007, pTyr1008
(34), (28), (14), (13), (11), (10), (8), (4), (4), (4), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human, Rat (Rattus)
(192), (156), (151), (38), (25), (24), (12), (2), (2), (1), (1)
Host
Rabbit
(194), (11), (2)
Clonality
Monoclonal
Conjugate
Un-conjugated
(8), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (1), (1)
Application
Western Blotting (WB), Immunohistochemistry (IHC), Dot Blot (DB)
(134), (88), (82), (30), (30), (18), (15), (11), (4), (2), (2)
Pubmed 3 references available
Quantity 100 µL
Options
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Catalog No. ABIN649956
507.14 $
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Specificity A synthetic phospho-peptide corresponding to residues surrounding Tyr1007 and Tyr1008 of human JAK2 was used as immunogen. The antibody only detects JAK2 phosphorylated on Tyrosine 1007 and 1008.
Alternative Name JAK2
Background Jak2 is a member of the Janus family tyrosine kinases (Jaks), whose function is to transduce extracellular signals from cytokines and interferons. Jaks associate with cytokine receptors, upon ligand binding, Jaks phosphorylate the receptor. The phosphorylated receptors are bound by SH2-containing proteins, one class of which is the signal transducers and activators of transcription (STATs). Activated STATs, then, translocate to the nucleus to mediate gene transcription (1,2). Tyr1007/1008 of Jak2 are the homologous Tyr1054/1055 in Tyk2, and phosphorylation at these residues is needed for Tyk2 activation (3). A chromosomal aberration involving Jak2 is found in a form of pre-B acute myeloid leukemia (4).
Synonyms: JAK2, Tyrosine-protein kinase JAK2, Janus kinase 2
Molecular Weight 130 kDA
Gene ID 3717
UniProt O60674
Research Area Ubiquitin-related antibodies, Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Structure, Cancer, Signaling, Chromatin
Application Notes WB: = 1:5000-200000 , IHC: = 1:50-100
Comment

Background: Jak2 is a member of the Janus family tyrosine kinases (Jaks), whose function is to transduce extracellular signals from cytokines and interferons. Jaks associate with cytokine receptors, upon ligand binding, Jaks phosphorylate the receptor. The phosphorylated receptors are bound by SH2-containing proteins, one class of which is the signal transducers and activators of transcription (STATs). Activated STATs, then, translocate to the nucleus to mediate gene transcription (1,2). Tyr1007/1008 of Jak2 are the homologous Tyr1054/1055 in Tyk2, and phosphorylation at these residues is needed for Tyk2 activation (3). A chromosomal aberration involving Jak2 is found in a form of pre-B acute myeloid leukemia (4).

Restrictions For Research Use only
Format Liquid
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment JAK2 Antibody Phospho (pY1007/1008) can be stored at -20°C for up to 12 months from time of receipt.
Expiry Date 12 months
Background publications Gauzzi, Velazquez, McKendry et al.: "Interferon-alpha-dependent activation of Tyk2 requires phosphorylation of positive regulatory tyrosines by another kinase." in: The Journal of biological chemistry, Vol. 271, Issue 34, pp. 20494-500, 1996 (PubMed).

Peeters, Raynaud, Cools et al.: "Fusion of TEL, the ETS-variant gene 6 (ETV6), to the receptor-associated kinase JAK2 as a result of t(9;12) in a lymphoid and t(9;15;12) in a myeloid leukemia." in: Blood, Vol. 90, Issue 7, pp. 2535-40, 1997 (PubMed).

OShea, Notarangelo, Johnston et al.: "Advances in the understanding of cytokine signal transduction: the role of Jaks and STATs in immunoregulation and the pathogenesis of immunodeficiency." in: Journal of clinical immunology, Vol. 17, Issue 6, pp. 431-47, 1998 (PubMed).

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