Tyrosine-Protein Kinase JAK3 (JAK3) antibody

Antigen: Tyrosine-Protein Kinase JAK3 (JAK3)

Synonyms: JAKL, LJAK, JAK-3, L-JAK, JAK3_HUMAN

Clonality: Monoclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC)

Certificates: ISO 9001:2008

Catalog no.: ABIN649957

Additional Information

Alternative name: JAK3

Gene ID: 4436

UniProt: P52333

Characteristics: Rabbit Monoclonal IgG

Specificity: A synthetic peptide corresponding to residues on the N-terminus in human JAK3 was used as an immunogen.

Molecular Weight: 115 kDA

Comments:

Background: JAK3 is a member of the Janus family (JAK) tyrosine kinases involved in cytokine receptor-mediated intracellular signal transduction. The JAK family is constitutively associated with cytokine receptors, upon binding to the receptor, the cytokine becomes phosphorylated (1). JAK also phosphorylates the cytokine receptor on tyrosine (Tyr) residues that serve as docking sites for cytoplasmic signal transducers and activators of transcription (STAT) proteins (2). Activated STATs translocate to the nucleus to mediate gene transcription (1). JAK3 is unique among JAK family members because it serves a function in normal lymphocyte development. In addition, JAK3 is the only one that interacts with the common gamma chain of the following Interleukin receptors: IL-2, IL-4, IL-7, IL-9, and IL-15 receptors (3). Loss of JAK3 function causes a severe block in T-cell and natural killer (NK) cell development (4). Defects in JAK3 are a cause of severe combined immunodeficiency (SCID), characterized by an absence of T-cell-mediated cellular immunity (5).

Application Details

Application Notes: The suggested dilution is: WB: ~~ 1:10000~20000 ICC: ~~ 1:250~500

Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage: JAK3 Antibody can be stored at -20°C for up to 12 months from time of receipt.

Research Area: Ubiquitin-related antibodies, Phospho-specific antibodies, Cell Signaling, Cell Structure, Protein Modifications, Signaling, Chromatin

Restrictions: For Research Use only

Publications

Product: Malabarba, Kirken, Rui et al.: "Activation of JAK3, but not JAK1, is critical to interleukin-4 (IL4) stimulated proliferation and requires a membrane-proximal region of IL4 receptor alpha." in: The Journal of biological chemistry, Vol. 270, Issue 16, pp. 9630-7, 1995 (PubMed).

Leonard, OShea: "Jaks and STATs: biological implications." in: Annual review of immunology, Vol. 16, pp. 293-322, 1998 (PubMed).

Pesu, Candotti, Husa et al.: "Jak3, severe combined immunodeficiency, and a new class of immunosuppressive drugs." in: Immunological reviews, Vol. 203, pp. 127-42, 2005 (PubMed).

Cornejo, Kharas, Werneck et al.: "Constitutive JAK3 activation induces lymphoproliferative syndromes in murine bone marrow transplantation models." in: Blood, Vol. 113, Issue 12, pp. 2746-54, 2009 (PubMed).

Vaysberg, Lambert, Krams et al.: "Activation of the JAK/STAT pathway in Epstein Barr virus+-associated posttransplant lymphoproliferative disease: role of interferon-gamma." in: American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons, Vol. 9, Issue 10, pp. 2292-302, 2009 (PubMed).