Tyrosine-Protein Kinase JAK3 (JAK3) (C-Term) antibody
| Antigen | Tyrosine-Protein Kinase JAK3 (JAK3) |
| Synonyms | JAKL, LJAK, JAK-3, L-JAK, JAK3_HUMAN |
| Binding Site |
 Alternatives C-Term |
| Clonality | Monoclonal |
| Host |
Alternatives Rabbit |
| Reactivity |
Alternatives Human |
| Conjugate |
Alternatives Un-conjugated |
| Application |
Alternatives Western Blotting (WB), Flow Cytometry (FACS), Immunoprecipitation (IP) |
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1 reference available |
| Certificates | ISO 9001:2008 |
| Catalog no. | ABIN649958 |
| Quantity | 100ul |
| Price | 450.00 $ Plus shipping costs $45.00 |
| Shipping to |
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| Availability | Will be delivered in 2 to 3 Business Days |
Additional Information
| Alternative name | JAK3 |
| Gene ID | 3718 |
| UniProt | P52333 |
| Description | Other names: JAK3, Tyrosine-protein kinase JAK3, Leukocyte janus kinase |
| Characteristics | Rabbit Monoclonal Antibody |
| Specificity | A synthetic peptide corresponding to residues on the C-terminus of human JAK3 was used as an immunogen. |
| Molecular Weight | 115 kDA |
| Comments |
Background: Jak3 is a member of the Janus family tyrosine kinases (Jaks), whose function is to transduce extracellular signals from cytokines and interferons. Jaks associate with cytokine receptors, upon ligand binding, Jaks phosphorylate the receptor. The phosphorylated receptors are bound by SH2-containing proteins, one class of which is the signal transducers and activators of transcription (STATs). Activated STATs translocate to the nucleus to mediate gene transcription (1, 2). Jak3 is activated after binding to the gamma chain of Interleukin receptors, specifically IL-2, IL-4, IL-7, IL-9, and IL-15 receptors. Jak3 has been implicated in mast cell-mediated immediate hypersensitivity reactions, autoimmune disorders, and acute lymphoblastic leukemia. Jak3 inhibitor has shown clinical potential as a new class of immunomodulatory agents with anti-leukemic and immunosuppressive properties (3). |
Application Details
| Application Notes | The suggested dilution is: WB: ~~ 1:2000 IP: ~~ 1:50 FC: ~~ 1:30 |
| Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
| Storage | JAK3 Antibody (C-term) can be stored at -20°C for up to 12 months from time of receipt. |
| Research Area | Ubiquitin-related antibodies, Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Structure, Chromatin, Signaling |
| Restrictions | For Research Use only |
Publications
| Product |
Leonard, OShea: "Jaks and STATs: biological implications." in: Annual review of immunology, Vol. 16, pp. 293-322, 1998 (PubMed).
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Alternatives
| Hosts | Rabbit (36), Mouse (7) |
| Reactivities | Human (41), Mouse (Murine) (36), Rat (Rattus) (34), Cow (Bovine) (29), Pig (Porcine) (29), Horse (Equine) (28), Dog (Canine) (24), Chicken (1) |
| Applications | Immunofluorescence (IF) (31), Western Blotting (WB) (15), ELISA (11), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (6), Immunocytochemistry (ICC) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Immunohistochemistry (IHC) (3), Immunoprecipitation (IP) (2), Flow Cytometry (FACS) (1), Immunoelectron Microscopy (IEM) (1) |
| Conjugates | Alexa Fluor 350 (3), Alexa Fluor 488 (3), Alexa Fluor 555 (3), Alexa Fluor 647 (3), PE,Cy5.5 (3), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy7 (1) |
| Epitopes | pTyr785 (6), pTyr981,pTyr980 (4), N-Term (1), pTyr221 (1) |
2013 BioMed Central Research Awards
