Mitogen-Activated Protein Kinase 9 (MAPK9) antibody

Details for Product No. ABIN649962
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Antigen
Synonyms MAPK9, JNK-55, JNK2, JNK2A, JNK2ALPHA, JNK2B, JNK2BETA, PRKM9, SAPK, SAPK1a, p54a, p54aSAPK, AI851083, Prkm9, JNK2-ALPHA1, MAP kinase 9
Reactivity
Human, Rat (Rattus)
(118), (65), (58), (17), (13), (13)
Host
Rabbit
(93), (29), (4), (3), (1)
Clonality
Monoclonal
Conjugate
Un-conjugated
(3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Immunohistochemistry (IHC)
(108), (55), (33), (31), (17), (13), (10), (5), (3), (1), (1), (1)
Pubmed 1 reference available
Quantity 100 µL
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Catalog No. ABIN649962
450.00 $
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Specificity A synthetic peptide corresponding to residues near the C-terminus of human JNK2 was used as an immunogen.
Alternative Name JNK2
Background JNK protein kinases are distantly related to mitogen-activated protein kinases (ERKs) and are activated by dual phosphorylation on Tyr and Thr. The JNK protein kinase group includes the 46-kDa isoform JNK1 and the 55-kDa protein kinase JNK2. The activities of both JNK isoforms are markedly increased by exposure of cells to UV radiation (1). JNK becomes activated in vivo in response to pro-inflammatory cytokines or cellular stresses. Its full activation requires the phosphorylation of a threonine and a tyrosine residue in a Thr-Pro-Tyr motif, which can be catalysed by the protein kinases mitogen-activated protein kinase kinase MKK4 and MKK7 (2). JNK2 functions in a cell-type-specific and stimulus-dependent manner, being required for apoptosis of immature thymocytes induced by anti-CD3 antibody but not for apoptosis induced by anti-Fas antibody, UVC or dexamethasone. JNK2 is not required for activation-induced cell death of mature T cells (3).
Synonyms: MAPK9, JNK2, PRKM9, Mitogen-activated protein kinase 9, Stress-activated protein kinase JNK2,c-Jun N-terminal kinase 2,JNK-55
Molecular Weight 54/46 kDA
Gene ID 5601
UniProt P45984
Research Area Phospho-specific antibodies, Cell Signaling, Protein Modifications, Signaling, Chromatin, Cell Structure
Application Notes IHC: = 1:100-250, WB: = 1:500
Comment

Background: JNK protein kinases are distantly related to mitogen-activated protein kinases (ERKs) and are activated by dual phosphorylation on Tyr and Thr. The JNK protein kinase group includes the 46-kDa isoform JNK1 and the 55-kDa protein kinase JNK2. The activities of both JNK isoforms are markedly increased by exposure of cells to UV radiation (1). JNK becomes activated in vivo in response to pro-inflammatory cytokines or cellular stresses. Its full activation requires the phosphorylation of a threonine and a tyrosine residue in a Thr-Pro-Tyr motif, which can be catalysed by the protein kinases mitogen-activated protein kinase kinase MKK4 and MKK7 (2). JNK2 functions in a cell-type-specific and stimulus-dependent manner, being required for apoptosis of immature thymocytes induced by anti-CD3 antibody but not for apoptosis induced by anti-Fas antibody, UVC or dexamethasone. JNK2 is not required for activation-induced cell death of mature T cells (3).

Restrictions For Research Use only
Format Liquid
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment JNK2 Antibody can be stored at -20°C for up to 12 months from time of receipt.
Expiry Date 12 months
Background publications Sluss, Barrett, Dérijard et al.: "Signal transduction by tumor necrosis factor mediated by JNK protein kinases." in: Molecular and cellular biology, Vol. 14, Issue 12, pp. 8376-84, 1994 (PubMed).

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