Mitogen-Activated Protein Kinase 9 (MAPK9) antibody

Antigen: Mitogen-Activated Protein Kinase 9 (MAPK9)

Synonyms: JNK2, SAPK, p54a, JNK2A, JNK2B, PRKM9, JNK-55, JNK2BETA, p54aSAPK, JNK2ALPHA, MAPK9, MGC137545, MAP kinase 9, MPK9

Clonality: Monoclonal

Host: Rabbit

Reactivity: Human, Rat (Rattus)

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunoprecipitation (IP)

Certificates: ISO 9001:2008

Catalog no.: ABIN649962

Additional Information

Alternative name: JNK2

Gene ID: 5601

UniProt: P45984

Description: Other names: MAPK9, JNK2, PRKM9, Mitogen-activated protein kinase 9, Stress-activated protein kinase JNK2,c-Jun N-terminal kinase 2,JNK-55

Characteristics: Rabbit Monoclonal Antibody

Specificity: A synthetic peptide corresponding to residues near the C-terminus of human JNK2 was used as an immunogen.

Molecular Weight: 54/46 kDA

Comments:

Background: JNK protein kinases are distantly related to mitogen-activated protein kinases (ERKs) and are activated by dual phosphorylation on Tyr and Thr. The JNK protein kinase group includes the 46-kDa isoform JNK1 and the 55-kDa protein kinase JNK2. The activities of both JNK isoforms are markedly increased by exposure of cells to UV radiation (1). JNK becomes activated in vivo in response to pro-inflammatory cytokines or cellular stresses. Its full activation requires the phosphorylation of a threonine and a tyrosine residue in a Thr-Pro-Tyr motif, which can be catalysed by the protein kinases mitogen-activated protein kinase kinase MKK4 and MKK7 (2). JNK2 functions in a cell-type-specific and stimulus-dependent manner, being required for apoptosis of immature thymocytes induced by anti-CD3 antibody but not for apoptosis induced by anti-Fas antibody, UVC or dexamethasone. JNK2 is not required for activation-induced cell death of mature T cells (3).

Application Details

Application Notes: The suggested dilution is: WB: ~~ 1:500 IP: ~~ 1:100 FC: ~~ 1:40 ICC: ~~ 1:100~250 IHC: ~~ 1:100~250

Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage: JNK2 Antibody can be stored at -20°C for up to 12 months from time of receipt.

Research Area: Phospho-specific antibodies, Cell Signaling, Protein Modifications, Signaling, Chromatin, Cell Structure

Restrictions: For Research Use only

Publications

Product: Sluss, Barrett, Dérijard et al.: "Signal transduction by tumor necrosis factor mediated by JNK protein kinases." in: Molecular and cellular biology, Vol. 14, Issue 12, pp. 8376-84, 1994 (PubMed).