Kinase insert Domain Receptor (A Type III Receptor tyrosine Kinase) (KDR) antibody

Antigen: Kinase insert Domain Receptor (A Type III Receptor tyrosine Kinase) (KDR)

Synonyms: Flk1, Ly73, Flk-1, Krd-1, VEGFR2, VEGFR-2, sVEGFR-2, 6130401C07, FLK1, CD309, VEGFR, Vegfr-2, MGC93590, KDR, FLK-1, flk-1, vegfr-2

Clonality: Monoclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Certificates: ISO 9001:2008

Catalog no.: ABIN649963

Additional Information

Alternative name: KDR (VEGFR-2)

Gene ID: 3791

UniProt: P35968

Description: Other names: KDR, FLK1, Vascular endothelial growth factor receptor 2, Kinase insert domain receptor,Protein-tyrosine kinase receptor Flk-1

Characteristics: Rabbit Monoclonal Antibody

Specificity: A synthetic peptide corresponding to residues in extracellular domain of human KDR/VEGFR2 was used as an immunogen. It is predicted that this antibody does not cross react with other members of VEGF receptor family.

Molecular Weight: 210/230 kDA

Comments:

Background: The expression of VEGF and KDR correlates highly with the normal ocular vascularization in humans, but VEGF may also be necessary for nonvascular retinal developmental functions, especially for the coordination of neural retinal development and the preliminary steps of the establishment of the definitive stable retinal vasculature (1). KDR/flk-1 is one of two receptors for vascular endothelial growth factor, a potent angiogenic peptide. KDR/flk-1 is an early marker for endothelial cell progenitors, and its expression is restricted to endothelial cells in vivo (2). Therapeutic angiogenesis is likely to require the administration of factors that complement each other. Activation of the receptor tyrosine kinase KDR/Flk1 by vascular endothelial growth factor (VEGF) is crucial, but molecular interactions of other factors with VEGF and Flk1 have been studied to a limited extent. Activation of KDR/Flt1 by PGF resulted in intermolecular transphosphorylation of Flk1, thereby amplifying VEGF-driven angiogenesis through KDR/Flk1. Even though VEGF and PGF both bind KDR/Flt1, PGF uniquely stimulated the phosphorylation of specific KDR/Flt1 tyrosine residues and the expression of distinct downstream target genes (3).

Application Details

Application Notes: The suggested dilution is: WB: ~~ 1:500 IP: ~~ 1:20

Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage: KDR (VEGFR-2) Antibody can be stored at -20°C for up to 12 months from time of receipt.

Research Area: Ubiquitin-related antibodies, Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Structure, Cell Cycle, Signaling, Chromatin

Restrictions: For Research Use only

Publications

Product: Autiero, Waltenberger, Communi et al.: "Role of PlGF in the intra- and intermolecular cross talk between the VEGF receptors Flt1 and Flk1." in: Nature medicine, Vol. 9, Issue 7, pp. 936-43, 2003 (PubMed).