Antigen Identified By Monoclonal Antibody Ki-67 (MKI67) antibody

Details for Product No. ABIN649965
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Antigen
Synonyms KIA, MIB-1, Ki67, Ki-67, D630048A14Rik, MKI67, kia, mki67, MGC132156
Reactivity
Human
(192), (33), (27), (2), (1), (1), (1)
Host
Rabbit
(116), (81), (6), (2)
Clonality
Monoclonal
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
(95), (73), (61), (54), (31), (23), (19), (10), (9), (7), (3), (1), (1), (1)
Pubmed 2 references available
Catalog no. ABIN649965
Quantity 100 µL
Price
450.00 $   Plus shipping costs $45.00
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Specificity A synthetic peptide corresponding to residues in human Ki-67 was used as an immunogen.
Alternative Name Ki-67
Background Ki-67 antigen is a nuclear antigen specifically associated with cell proliferation. Ki-67 is expressed in all proliferating cells which are in the active phases of the cell cycle (late G1, S, G2, and mitosis), but absent in resting cells. In G1, it is predominately localized in the perinucleolar region and also found in nuclear matrix in the later phases (1). Ki-67 is thought to be involved in the maintaining of cell proliferation, however, its exact mechanism for function is unknown. Due to the fact that Ki-67 can be used to determine the growth fraction (the proportion of the cells committed to the cell cycle), it is consider an important marker in human tumor diagnostics and prognostics (2).
Molecular Weight 395 kDA
Gene ID 12576
UniProt P46013
Research Area Signaling, Phospho-specific antibodies, Protein Modifications, Chromatin, Cell Structure
Application Notes WB: = 1:2000-10000 , IHC: = 1:500-1000
Comment

Background: Ki-67 antigen is a nuclear antigen specifically associated with cell proliferation. Ki-67 is expressed in all proliferating cells which are in the active phases of the cell cycle (late G1, S, G2, and mitosis), but absent in resting cells. In G1, it is predominately localized in the perinucleolar region and also found in nuclear matrix in the later phases (1). Ki-67 is thought to be involved in the maintaining of cell proliferation, however, its exact mechanism for function is unknown. Due to the fact that Ki-67 can be used to determine the growth fraction (the proportion of the cells committed to the cell cycle), it is consider an important marker in human tumor diagnostics and prognostics (2).

Restrictions For Research Use only
Format Liquid
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Preservative Sodium azide
Storage -20 °C
Storage Comment Ki-67 Antibody can be stored at -20°C for up to 12 months from time of receipt.
Expiry Date 12 months
Background publications Gerdes: "Ki-67 and other proliferation markers useful for immunohistological diagnostic and prognostic evaluations in human malignancies." in: Seminars in cancer biology, Vol. 1, Issue 3, pp. 199-206, 1991 (PubMed).

Scholzen, Gerdes: "The Ki-67 protein: from the known and the unknown." in: Journal of cellular physiology, Vol. 182, Issue 3, pp. 311-22, 2000 (PubMed).

Hosts (116), (81), (6), (2)
Reactivities (192), (33), (27), (2), (1), (1), (1)
Applications (95), (73), (61), (54), (31), (23), (19), (10), (9), (7), (3), (1), (1), (1)
Conjugates (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (25), (8), (5), (4), (2), (2), (1), (1), (1), (1), (1), (1), (1)
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