Antigen Identified By Monoclonal Antibody Ki-67 (MKI67) antibody

Antigen: Antigen Identified By Monoclonal Antibody Ki-67 (MKI67)

Synonyms: KIA, Ki67, Ki-67, D630048A14Rik, MKI67, kia, mki67, MGC132156

Clonality: Monoclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Certificates: ISO 9001:2008

Catalog no.: ABIN649965

Additional Information

Alternative name: Ki-67

Gene ID: 12576

UniProt: P46013

Characteristics: Rabbit Monoclonal Antibody

Specificity: A synthetic peptide corresponding to residues in human Ki-67 was used as an immunogen.

Molecular Weight: 395 kDA

Comments:

Background: Ki-67 antigen is a nuclear antigen specifically associated with cell proliferation. Ki-67 is expressed in all proliferating cells which are in the active phases of the cell cycle (late G1, S, G2, and mitosis), but absent in resting cells. In G1, it is predominately localized in the perinucleolar region and also found in nuclear matrix in the later phases (1). Ki-67 is thought to be involved in the maintaining of cell proliferation, however, its exact mechanism for function is unknown. Due to the fact that Ki-67 can be used to determine the growth fraction (the proportion of the cells committed to the cell cycle), it is consider an important marker in human tumor diagnostics and prognostics (2).

Application Details

Application Notes: The suggested dilution is: WB: ~~ 1:2000~10000 IP: ~~ 1:30 FC: ~~ 1:60 ICC: ~~ 1:250~500 IHC: ~~ 1:500~1000

Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage: Ki-67 Antibody can be stored at -20°C for up to 12 months from time of receipt.

Research Area: Signaling, Phospho-specific antibodies, Protein Modifications, Chromatin, Cell Structure

Restrictions: For Research Use only

Publications

Product: Gerdes: "Ki-67 and other proliferation markers useful for immunohistological diagnostic and prognostic evaluations in human malignancies." in: Seminars in cancer biology, Vol. 1, Issue 3, pp. 199-206, 1991 (PubMed).

Scholzen, Gerdes: "The Ki-67 protein: from the known and the unknown." in: Journal of cellular physiology, Vol. 182, Issue 3, pp. 311-22, 2000 (PubMed).