Kinesin Family Member 22 (KIF22) (pSer427) antibody

Antigen: Kinesin Family Member 22 (KIF22)

Synonyms: KID, OBP, KNSL4, OBP-1, OBP-2, KIF22, MGC148851, T1E22.130, T1E22_130, DKFZp459I1739

Binding Site: pSer427

Clonality: Monoclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN649968

Additional Information

Alternative name: KID

Gene ID: 3835

UniProt: Q14807

Description: Other names: KIF22, KID, KNSL4, Kinesin-like protein KIF22, Kinesin-like DNA-binding protein,Kinesin-like protein 4

Characteristics: Rabbit Monoclonal Antibody

Specificity: A phospho-specific peptide corresponding to residues surrounding Serine 427 of human KID was used as an immunogen. This antibody only detects KID phosphorylated on Serine 427.

Molecular Weight: 73 kDA

Comments:

Background: KID (KNSL4, kinesin-like DNA-binding protein) is a member of the kinesin family that is involved in spindle formation and the movements of chromosomes during mitosis and meiosis (1). KID colocalizes with mitotic chromosomes and is enriched in the kinetochore at anaphase. The N-terminal region of KID shares greater than 35% sequence homology with the motor domains of other kinesins. The C-terminal domain has limited but significant homology to NOD, a Drosophila protein and member of the kinesin family. KID also contains a putative nuclear localization signal (2). It was demonstrated that SIAH, a human homologue of the Drosophila seven in absentia (Sina), is involved in the degradation of KID via the ubiquitin-proteasome pathway (3). It was also reported that KID function is regulated by phosphorylation. Ser427 and Thr463 were identified as M phase-specific phosphorylation sites (4).

Application Details

Application Notes: The suggested dilution is: WB: ~~ 1:1000~2000

Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage: KID Antibody Phospho (pS427) can be stored at -20°C for up to 12 months from time of receipt.

Research Area: Ubiquitin-related antibodies, Phospho-specific antibodies, Protein Modifications, Transcription Factors, Signaling, Chromatin, Cell Structure

Restrictions: For Research Use only

Publications

Product: Tokai, Fujimoto-Nishiyama, Toyoshima et al.: "Kid, a novel kinesin-like DNA binding protein, is localized to chromosomes and the mitotic spindle." in: The EMBO journal, Vol. 15, Issue 3, pp. 457-67, 1996 (PubMed).

Song, Murakami, Yang et al.: "Human genes for KNSL4 and MAZ are located close to one another on chromosome 16p11.2." in: Genomics, Vol. 52, Issue 3, pp. 374-7, 1998 (PubMed).

Germani, Bruzzoni-Giovanelli, Fellous et al.: "SIAH-1 interacts with alpha-tubulin and degrades the kinesin Kid by the proteasome pathway during mitosis." in: Oncogene, Vol. 19, Issue 52, pp. 5997-6006, 2001 (PubMed).