Kinesin Family Member 22 (KIF22) (pSer427) antibody

Details for Product No. ABIN649968
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Antigen
Synonyms A-328A3.2, KID, KNSL4, OBP, OBP-1, OBP-2, SEMDJL2, AU021460, C81217, Kid, Kif22a, KIF22, T1E22.130, T1E22_130, DKFZp459I1739
Epitope
pSer427
(7), (2), (1), (1)
Reactivity
Human
(27), (4), (4), (2), (2)
Host
Rabbit
(16), (14)
Clonality
Monoclonal
Application
Western Blotting (WB)
(27), (15), (13), (2), (2), (2), (2), (1), (1), (1), (1)
Pubmed 3 references available
Catalog no. ABIN649968
Quantity 100 µL
Price
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Specificity A phospho-specific peptide corresponding to residues surrounding Serine 427 of human KID was used as an immunogen. This antibody only detects KID phosphorylated on Serine 427.
Alternative Name KID
Background KID (KNSL4, kinesin-like DNA-binding protein) is a member of the kinesin family that is involved in spindle formation and the movements of chromosomes during mitosis and meiosis (1). KID colocalizes with mitotic chromosomes and is enriched in the kinetochore at anaphase. The N-terminal region of KID shares greater than 35% sequence homology with the motor domains of other kinesins. The C-terminal domain has limited but significant homology to NOD, a Drosophila protein and member of the kinesin family. KID also contains a putative nuclear localization signal (2). It was demonstrated that SIAH, a human homologue of the Drosophila seven in absentia (Sina), is involved in the degradation of KID via the ubiquitin-proteasome pathway (3). It was also reported that KID function is regulated by phosphorylation. Ser427 and Thr463 were identified as M phase-specific phosphorylation sites (4).
Synonyms: KIF22, KID, KNSL4, Kinesin-like protein KIF22, Kinesin-like DNA-binding protein,Kinesin-like protein 4
Molecular Weight 73 kDA
Gene ID 3835
UniProt Q14807
Research Area Ubiquitin-related antibodies, Phospho-specific antibodies, Protein Modifications, Transcription Factors, Signaling, Chromatin, Cell Structure
Application Notes The suggested dilution is: WB: = 1:1000-2000
Comment

Background: KID (KNSL4, kinesin-like DNA-binding protein) is a member of the kinesin family that is involved in spindle formation and the movements of chromosomes during mitosis and meiosis (1). KID colocalizes with mitotic chromosomes and is enriched in the kinetochore at anaphase. The N-terminal region of KID shares greater than 35% sequence homology with the motor domains of other kinesins. The C-terminal domain has limited but significant homology to NOD, a Drosophila protein and member of the kinesin family. KID also contains a putative nuclear localization signal (2). It was demonstrated that SIAH, a human homologue of the Drosophila seven in absentia (Sina), is involved in the degradation of KID via the ubiquitin-proteasome pathway (3). It was also reported that KID function is regulated by phosphorylation. Ser427 and Thr463 were identified as M phase-specific phosphorylation sites (4).

Restrictions For Research Use only
Format Liquid
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Preservative Sodium azide
Storage -20 °C
Storage Comment KID Antibody Phospho (pS427) can be stored at -20°C for up to 12 months from time of receipt.
Expiry Date 12 months
Background publications Tokai, Fujimoto-Nishiyama, Toyoshima et al.: "Kid, a novel kinesin-like DNA binding protein, is localized to chromosomes and the mitotic spindle." in: The EMBO journal, Vol. 15, Issue 3, pp. 457-67, 1996 (PubMed).

Song, Murakami, Yang et al.: "Human genes for KNSL4 and MAZ are located close to one another on chromosome 16p11.2." in: Genomics, Vol. 52, Issue 3, pp. 374-7, 1998 (PubMed).

Germani, Bruzzoni-Giovanelli, Fellous et al.: "SIAH-1 interacts with alpha-tubulin and degrades the kinesin Kid by the proteasome pathway during mitosis." in: Oncogene, Vol. 19, Issue 52, pp. 5997-6006, 2001 (PubMed).

Hosts (16), (14)
Reactivities (27), (4), (4), (2), (2)
Applications (27), (15), (13), (2), (2), (2), (2), (1), (1), (1), (1)
Epitopes (7), (2), (1), (1)
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Validation Images
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