X-Ray Repair Complementing Defective Repair in Chinese Hamster Cells 6 (XRCC6) antibody

Antigen: X-Ray Repair Complementing Defective Repair in Chinese Hamster Cells 6 (XRCC6)

Synonyms: ML8, KU70, TLAA, CTC75, CTCBF, G22P1, XRCC6, MGC82304, DKFZp459H0114

Clonality: Monoclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunoprecipitation (IP)

Certificates: ISO 9001:2008

Catalog no.: ABIN649971

Additional Information

Alternative name: Ku70

Gene ID: 1152

UniProt: P12956

Characteristics: Rabbit Monoclonal IgG

Specificity: A synthetic peptide corresponding to residues in human Ku70 was used as an immunogen.

Molecular Weight: 70 kDA

Comments:

Background: ATP-dependent DNA helicase 2 subunit 1 (Ku70) is a single stranded DNA-dependent helicase originally recognized by the sera of patients with autoimmune diseases (1, 2). It plays a key role in multiple nuclear processes such as DNA repair, chromosome maintenance, transcription regulation, and V(D)J recombination (3). Ku70 forms a heterodimer with Ku80 and contributes to genomic integrity through its ability to bind DNA double-strand breaks and facilitate repair by the non-homologous end-joining (NHEJ) pathway (4). The dimer associates in a DNA-dependent manner with PRKDC to form the DNA-dependent protein kinase complex, DNA-PK (1). Together, the Ku70/Ku80 heterodimer and DNA-PKcs are required for V(D)J recombination and DNA double-strand break repair and may also play a role in transcription regulation (5). In addition, the dimer associates with NARG1 to bind to the osteocalcin promoter and activate osteocalcin expression (1).

Application Details

Application Notes: The suggested dilution is: WB: ~~ 1:2000~10000 IP: ~~ 1:10~100 FC: ~~ 1:10~100 ICC: ~~ 1:100~250 IHC: ~~ 1:100~250

Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage: Ku70 Antibody can be stored at -20°C for up to 12 months from time of receipt.

Research Area: Signaling, Phospho-specific antibodies, Protein Modifications, Transcription Factors, Chromatin, Metabolism, Cell Structure

Restrictions: For Research Use only

Publications

Product: Yaneva, Wen, Ayala et al.: "cDNA-derived amino acid sequence of the 86-kDa subunit of the Ku antigen." in: The Journal of biological chemistry, Vol. 264, Issue 23, pp. 13407-11, 1989 (PubMed).

Chan, Ye, Veillette et al.: "DNA-dependent protein kinase phosphorylation sites in Ku 70/80 heterodimer." in: Biochemistry, Vol. 38, Issue 6, pp. 1819-28, 1999 (PubMed).

Walker, Corpina, Goldberg: "Structure of the Ku heterodimer bound to DNA and its implications for double-strand break repair." in: Nature, Vol. 412, Issue 6847, pp. 607-14, 2001 (PubMed).

Koike: "Dimerization, translocation and localization of Ku70 and Ku80 proteins." in: Journal of radiation research, Vol. 43, Issue 3, pp. 223-36, 2003 (PubMed).

"The Universal Protein Resource (UniProt) 2009." in: Nucleic acids research, Vol. 37, Issue Database issue, pp. D169-74, 2008 (PubMed).