X-Ray Repair Complementing Defective Repair in Chinese Hamster Cells 5 (Double-Strand-Break Rejoining) (XRCC5) antibody

Antigen: X-Ray Repair Complementing Defective Repair in Chinese Hamster Cells 5 (Double-Strand-Break Rejoining) (XRCC5)

Synonyms: XRCC5, MGC179425, DKFZp469K071

Clonality: Monoclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Certificates: ISO 9001:2008

Catalog no.: ABIN649972

Additional Information

Alternative name: Ku80

Gene ID: 7520

UniProt: P13010

Description: Other names: XRCC5, G22P2, ATP-dependent DNA helicase 2 subunit 2, Nuclear factor IV,DNA repair protein XRCC5

Characteristics: Rabbit Monoclonal Antibody

Specificity: A synthetic peptide corresponding to residues in human Ku80 was used as an immunogen.

Molecular Weight: 86 kDA

Comments:

Background: The Ku antigen is a DNA-associated nuclear protein originally recognized by the sera of patients with autoimmune diseases (1). It plays a key role in multiple nuclear processes, such as DNA repair, chromosome maintenance, transcription regulation, and V(D)J recombination (2). The Ku protein is composed of 70 and 80 kDa subunits (Ku70 and Ku80, respectively) and contributes to genomic integrity through its ability to bind DNA double-strand breaks and facilitate repair by the non-homologous end-joining pathway (3). When bound to DNA, the Ku 70/80 heterodimer enhances the kinase activity of the catalytic subunit of the DNA-dependent protein kinase, DNA-PKcs (4).

Application Details

Application Notes: The suggested dilution is: WB: ~~ 1:10000 FC: ~~ 1:160 ICC: ~~ 1:100~250 IHC: ~~ 1:250~500

Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage: Ku80 Antibody can be stored at -20°C for up to 12 months from time of receipt.

Research Area: Ubiquitin-related antibodies, Signaling, Phospho-specific antibodies, Protein Modifications, Transcription Factors, Chromatin, Metabolism, Cell Structure

Restrictions: For Research Use only

Publications

Product: Yaneva, Wen, Ayala et al.: "cDNA-derived amino acid sequence of the 86-kDa subunit of the Ku antigen." in: The Journal of biological chemistry, Vol. 264, Issue 23, pp. 13407-11, 1989 (PubMed).

Chan, Ye, Veillette et al.: "DNA-dependent protein kinase phosphorylation sites in Ku 70/80 heterodimer." in: Biochemistry, Vol. 38, Issue 6, pp. 1819-28, 1999 (PubMed).