X-Ray Repair Complementing Defective Repair in Chinese Hamster Cells 5 (Double-Strand-Break Rejoining) (XRCC5) antibody

Details for Product No. ABIN649972
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Antigen
Synonyms AI314015, Ku80, Ku86, Kup80, KARP-1, KARP1, KU80, KUB2, NFIV, xrcc5-a, XRCC5
Reactivity
Human
(188), (43), (27), (6), (4), (4), (1)
Host
Rabbit
(120), (65), (10)
Clonality
Monoclonal
Conjugate
Un-conjugated
(5), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Immunohistochemistry (IHC)
(158), (73), (54), (54), (53), (30), (25), (23), (10), (2), (2), (1), (1), (1), (1)
Pubmed 2 references available
Quantity 100 µL
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Catalog No. ABIN649972
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Specificity A synthetic peptide corresponding to residues in human Ku80 was used as an immunogen.
Alternative Name Ku80
Background The Ku antigen is a DNA-associated nuclear protein originally recognized by the sera of patients with autoimmune diseases (1). It plays a key role in multiple nuclear processes, such as DNA repair, chromosome maintenance, transcription regulation, and V(D)J recombination (2). The Ku protein is composed of 70 and 80 kDa subunits (Ku70 and Ku80, respectively) and contributes to genomic integrity through its ability to bind DNA double-strand breaks and facilitate repair by the non-homologous end-joining pathway (3). When bound to DNA, the Ku 70/80 heterodimer enhances the kinase activity of the catalytic subunit of the DNA-dependent protein kinase, DNA-PKcs (4).
Synonyms: XRCC5, G22P2, ATP-dependent DNA helicase 2 subunit 2, Nuclear factor IV,DNA repair protein XRCC5
Molecular Weight 86 kDA
Gene ID 7520
UniProt P13010
Research Area Ubiquitin-related antibodies, Signaling, Phospho-specific antibodies, Protein Modifications, Transcription Factors, Chromatin, Metabolism, Cell Structure
Application Notes IHC: = 1:250-500, WB: = 1:10000
Comment

Background: The Ku antigen is a DNA-associated nuclear protein originally recognized by the sera of patients with autoimmune diseases (1). It plays a key role in multiple nuclear processes, such as DNA repair, chromosome maintenance, transcription regulation, and V(D)J recombination (2). The Ku protein is composed of 70 and 80 kDa subunits (Ku70 and Ku80, respectively) and contributes to genomic integrity through its ability to bind DNA double-strand breaks and facilitate repair by the non-homologous end-joining pathway (3). When bound to DNA, the Ku 70/80 heterodimer enhances the kinase activity of the catalytic subunit of the DNA-dependent protein kinase, DNA-PKcs (4).

Restrictions For Research Use only
Format Liquid
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Ku80 Antibody can be stored at -20°C for up to 12 months from time of receipt.
Expiry Date 12 months
Background publications Yaneva, Wen, Ayala et al.: "cDNA-derived amino acid sequence of the 86-kDa subunit of the Ku antigen." in: The Journal of biological chemistry, Vol. 264, Issue 23, pp. 13407-11, 1989 (PubMed).

Chan, Ye, Veillette et al.: "DNA-dependent protein kinase phosphorylation sites in Ku 70/80 heterodimer." in: Biochemistry, Vol. 38, Issue 6, pp. 1819-28, 1999 (PubMed).

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