Search, Find and Buy Antibodies, ELISA Kits and Proteins.
Loading Loading
Order hotline:
phone +1 404 474 4654
fax +1 888 205 9894 (TF)
Details for Product No. ABIN649973

X-Ray Repair Complementing Defective Repair in Chinese Hamster Cells 5 (Double-Strand-Break Rejoining) (XRCC5) antibody

Request
Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Antigen
Synonyms XRCC5, xrcc5-a, KARP-1, KARP1, KU80, KUB2, Ku86, NFIV, AI314015, Ku80, Kup80
Reactivity
»Alternatives Human
Host
»Alternatives Rabbit
Clonality Monoclonal
Conjugate
»Alternatives Un-conjugated
Application
»Alternatives Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)
Pubmed 2 references available
Catalog no. ABIN649973
Quantity 100 µL
Price
450.00 $   Plus shipping costs $45.00
Shipping to
Availability Will be delivered in 2 to 3 Business Days
Specificity A synthetic peptide corresponding to residues in human Ku80 was used as an immunogen.
Alternative Name Ku80
Background The Ku antigen is a DNA-associated nuclear protein originally recognized by the sera of patients with autoimmune diseases (1). It plays a key role in multiple nuclear processes, such as DNA repair, chromosome maintenance, transcription regulation, and V(D)J recombination (2). The Ku protein is composed of 70 and 80 kDa subunits (Ku70 and Ku80, respectively) and contributes to genomic integrity through its ability to bind DNA double-strand breaks and facilitate repair by the non-homologous end-joining pathway (3). When bound to DNA, the Ku 70/80 heterodimer enhances the kinase activity of the catalytic subunit of the DNA-dependent protein kinase, DNA-PKcs (4).
Synonyms: XRCC5, G22P2, ATP-dependent DNA helicase 2 subunit 2, Nuclear factor IV,DNA repair protein XRCC5
Molecular Weight 86 kDA
Gene ID 7520
UniProt P13010
Research Area Ubiquitin-related antibodies, Signaling, Phospho-specific antibodies, Protein Modifications, Transcription Factors, Chromatin, Metabolism, Cell Structure
Application Notes The suggested dilution is: WB: ~~ 1:10,000 - 20,000 IP: ~~ 1:50 ICC: ~~ 1:500~1000 IHC: ~~ 1:250~500
Comment

Background: The Ku antigen is a DNA-associated nuclear protein originally recognized by the sera of patients with autoimmune diseases (1). It plays a key role in multiple nuclear processes, such as DNA repair, chromosome maintenance, transcription regulation, and V(D)J recombination (2). The Ku protein is composed of 70 and 80 kDa subunits (Ku70 and Ku80, respectively) and contributes to genomic integrity through its ability to bind DNA double-strand breaks and facilitate repair by the non-homologous end-joining pathway (3). When bound to DNA, the Ku 70/80 heterodimer enhances the kinase activity of the catalytic subunit of the DNA-dependent protein kinase, DNA-PKcs (4).

Restrictions For Research Use only
Format Liquid
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Preservative Sodium azide
Storage -20 °C
Storage Comment Ku80 Antibody can be stored at -20°C for up to 12 months from time of receipt.
Expiry Date 12 months
Background publications Yaneva, Wen, Ayala et al.: "cDNA-derived amino acid sequence of the 86-kDa subunit of the Ku antigen." in: The Journal of biological chemistry, Vol. 264, Issue 23, pp. 13407-11, 1989 (PubMed).

Chan, Ye, Veillette et al.: "DNA-dependent protein kinase phosphorylation sites in Ku 70/80 heterodimer." in: Biochemistry, Vol. 38, Issue 6, pp. 1819-28, 1999 (PubMed).

Alternatives for antigen "X-Ray Repair Complementing Defective Repair in Chinese Hamster Cells 5 (Double-Strand-Break Rejoining) (XRCC5)", type "Antibodies"
Hosts (108), (88), (12)
Reactivities (200), (43), (30), (7), (4), (4), (1)
Applications (163), (66), (64), (52), (38), (29), (26), (22), (10), (6), (3), (2), (2), (1), (1), (1)
Conjugates (7), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (18), (14), (5), (4), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Beacon