Lamin B Receptor Receptor antibody

Antigen: Lamin B Receptor Receptor

Clonality: Monoclonal

Host: Rabbit

Reactivity: Human, Rat (Rattus)

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Certificates: ISO 9001:2008

Catalog no.: ABIN649975

Additional Information

Gene ID: 3930

UniProt: Q14739

Description: Other names: LBR, Lamin-B receptor, Integral nuclear envelope inner membrane protein,LMN2R

Characteristics: Rabbit Monoclonal Antibody

Specificity: A synthetic peptide corresponding to residues of human Lamin B Receptor was used as immunogen.

Molecular Weight: 71 kDA

Comments:

Background: Lamins are nuclear membrane proteins that serve to maintain specific cellular functions, such as DNA replication and chromatin organization (1). Lamin B receptor (LBR) is an integral protein of the nuclear envelope inner membrane. It is phosphorylated by CDC2 protein kinase in mitosis when the inner nuclear membrane breaks down into vesicles that dissociate from the lamina and the chromatin. It is phosphorylated by different protein kinases in interphase when the membrane is associated with these structures (2). The cleavage of lamins results in nuclear disregulation and cell death (3,4).

Application Details

Application Notes: The suggested dilution is: WB: ~~ 1:5000 IP: ~~ 1:60 ICC: ~~ 1:500

Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage: Lamin B Receptor Antibody Receptor can be stored at -20°C for up to 12 months from time of receipt.

Research Area: Signaling, Phospho-specific antibodies, Cell Signaling, Cell Structure, Protein Modifications, Transcription Factors

Restrictions: For Research Use only

Publications

Product: Oberhammer, Hochegger, Fröschl et al.: "Chromatin condensation during apoptosis is accompanied by degradation of lamin A+B, without enhanced activation of cdc2 kinase." in: The Journal of cell biology, Vol. 126, Issue 4, pp. 827-37, 1994 (PubMed).

Ye, Worman: "Primary structure analysis and lamin B and DNA binding of human LBR, an integral protein of the nuclear envelope inner membrane." in: The Journal of biological chemistry, Vol. 269, Issue 15, pp. 11306-11, 1994 (PubMed).

Rao, Perez, White: "Lamin proteolysis facilitates nuclear events during apoptosis." in: The Journal of cell biology, Vol. 135, Issue 6 Pt 1, pp. 1441-55, 1997 (PubMed).