Linker For Activation of T Cells (LAT) antibody
| Antigen |
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| Synonyms |
LAT1, pp36, MGC108666, LAT, MGC157208, lat |
| Clonality |
Monoclonal |
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Host
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Reactivity
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Conjugate
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Alexa Fluor 488 (5), PE (5), Alexa Fluor 350 (3), Alexa Fluor 555 (3), Alexa Fluor 647 (3), Biotin (3), Cy3 (3), Cy5 (3), Cy5.5 (3), Cy7 (3), FITC (3), Gold (3), HRP (3), PE-Cy3 (3), PE-Cy5 (3), PE-Cy5.5 (3), PE-Cy7 (3), APC (2), Alexa Fluor 700 (2), Dyomics 647 (2)
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Application
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 Alternatives Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunoprecipitation (IP)
Western Blotting (WB) (84), Immunofluorescence (IF) (47), ELISA (45), Immunoprecipitation (IP) (33), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (20), Flow Cytometry (FACS) (16), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (9), Immunohistochemistry (IHC) (8), Immunocytochemistry (ICC) (7), Immunoelectron Microscopy (IEM) (3), Immunohistochemistry (Frozen Sections) (IHC (fro)) (2), Dot Blot (DB) (1)
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| Catalog no. |
ABIN649976 |
| Quantity |
100ul |
| Price |
450.00 $ Plus shipping costs $45.00
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Bulk discount
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| Shipping to |
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| Availability |
Will be delivered in 2 to 3 Business Days |
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Alternative name
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LAT
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Gene ID
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27040
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UniProt
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O43561
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Characteristics
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Rabbit Monoclonal Antibody
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Specificity
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A synthetic peptide corresponding to residues surrounding GRB2-binding motif 2 of human LAT was used as immunogen. This antibody detects both long and short forms of LAT.
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Molecular Weight
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36/38 kDA
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Comments
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Background: LAT (linker for activation of T-cells) is involved in the T-cell antigen receptor (TCR) signal transduction pathway, and may play an important role downstream in activating protein tyrosine kinases (1). LAT is phosphorylated by ZAP-70/Syk protein tyrosine kinases leading to recruitment of multiple signaling molecules (2). It has been found that Tyr191 and Tyr171 are required for T-cell activation and Tyr132 is required for the activation of PLCgamma1 and Ras signaling pathways, respectively. Tyr226 and Tyr191 are required for Vav binding, whereas Tyr171 and Tyr132 are necessary for association and activation of phosphoinositide 3-kinase activity and PLCgamma1 (3).
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Application Notes
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The suggested dilution is: WB: ~~ 1:500 IP: ~~ 1:60 FC: ~~ 1:40 IHC: ~~ 1:100
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Buffer
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50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
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Storage
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LAT Antibody can be stored at -20°C for up to 12 months from time of receipt.
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Research Area
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Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Structure
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Restrictions
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For Research Use only
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Product
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Zhang, Sloan-Lancaster, Kitchen et al.: "LAT: the ZAP-70 tyrosine kinase substrate that links T cell receptor to cellular activation." in: Cell, Vol. 92, Issue 1, pp. 83-92, 1998 (PubMed).
Weber, Orstavik, Torgersen et al.: "Molecular cloning of the cDNA encoding pp36, a tyrosine-phosphorylated adaptor protein selectively expressed by T cells and natural killer cells." in: The Journal of experimental medicine, Vol. 187, Issue 7, pp. 1157-61, 1998 (PubMed).
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Alternatives for antigen "Linker For Activation of T Cells (LAT)", type "Antibodies"
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Hosts
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Rabbit (101), Mouse (37), Goat (4), Sheep (1)
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Reactivities
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Human (136), Rat (Rattus) (79), Mouse (Murine) (76), Chicken (19), Cow (Bovine) (19), Dog (Canine) (19), Rabbit (18), Cat (Feline) (1)
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Applications
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Western Blotting (WB) (84), Immunofluorescence (IF) (47), ELISA (45), Immunoprecipitation (IP) (33), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (20), Flow Cytometry (FACS) (16), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (9), Immunohistochemistry (IHC) (8), Immunocytochemistry (ICC) (7), Immunoelectron Microscopy (IEM) (3), Immunohistochemistry (Frozen Sections) (IHC (fro)) (2), Dot Blot (DB) (1)
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Conjugates
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Alexa Fluor 488 (5), PE (5), Alexa Fluor 350 (3), Alexa Fluor 555 (3), Alexa Fluor 647 (3), Biotin (3), Cy3 (3), Cy5 (3), Cy5.5 (3), Cy7 (3), FITC (3), Gold (3), HRP (3), PE-Cy3 (3), PE-Cy5 (3), PE-Cy5.5 (3), PE-Cy7 (3), APC (2), Alexa Fluor 700 (2), Dyomics 647 (2)
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Epitopes
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C-Term (30), pTyr200 (20), pTyr161 (19), Cytoplasmic Domain (11), Internal Region (4), pTyr191 (4), AA 31-233 (3), pTyr220,C-Term (3), pTyr171 (2), AA 30-113 (1), AA 70-120 (1), C-Term,AA 191-221 (1), N-Term (1), pTyr132 (1), pTyr226 (1)
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