Linker For Activation of T Cells (LAT) antibody

Details for Product No. ABIN649976
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Antigen
Synonyms LAT, lat, LAT1, pp36
Reactivity
Human
(164), (75), (74), (36), (24), (12), (1)
Host
Rabbit
(116), (48), (6), (1)
Clonality
Monoclonal
Conjugate
Un-conjugated
(4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (2), (1), (1)
Application
Western Blotting (WB), Immunohistochemistry (IHC)
(125), (67), (30), (28), (25), (19), (13), (9), (5), (2), (2), (1), (1), (1)
Pubmed 2 references available
Quantity 100 μL
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Catalog No. ABIN649976
450.00 $
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Specificity A synthetic peptide corresponding to residues surrounding GRB2-binding motif 2 of human LAT was used as immunogen. This antibody detects both long and short forms of LAT.
Alternative Name LAT
Background LAT (linker for activation of T-cells) is involved in the T-cell antigen receptor (TCR) signal transduction pathway, and may play an important role downstream in activating protein tyrosine kinases (1). LAT is phosphorylated by ZAP-70/Syk protein tyrosine kinases leading to recruitment of multiple signaling molecules (2). It has been found that Tyr191 and Tyr171 are required for T-cell activation and Tyr132 is required for the activation of PLCgamma1 and Ras signaling pathways, respectively. Tyr226 and Tyr191 are required for Vav binding, whereas Tyr171 and Tyr132 are necessary for association and activation of phosphoinositide 3-kinase activity and PLCgamma1 (3).
Molecular Weight 36/38 kDA
Gene ID 27040
UniProt O43561
Research Area Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Structure
Application Notes IHC: = 1:100, WB: = 1:500
Comment

Background: LAT (linker for activation of T-cells) is involved in the T-cell antigen receptor (TCR) signal transduction pathway, and may play an important role downstream in activating protein tyrosine kinases (1). LAT is phosphorylated by ZAP-70/Syk protein tyrosine kinases leading to recruitment of multiple signaling molecules (2). It has been found that Tyr191 and Tyr171 are required for T-cell activation and Tyr132 is required for the activation of PLCgamma1 and Ras signaling pathways, respectively. Tyr226 and Tyr191 are required for Vav binding, whereas Tyr171 and Tyr132 are necessary for association and activation of phosphoinositide 3-kinase activity and PLCgamma1 (3).

Restrictions For Research Use only
Format Liquid
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment LAT Antibody can be stored at -20°C for up to 12 months from time of receipt.
Expiry Date 12 months
Background publications Zhang, Sloan-Lancaster, Kitchen et al.: "LAT: the ZAP-70 tyrosine kinase substrate that links T cell receptor to cellular activation." in: Cell, Vol. 92, Issue 1, pp. 83-92, 1998 (PubMed).

Weber, Orstavik, Torgersen et al.: "Molecular cloning of the cDNA encoding pp36, a tyrosine-phosphorylated adaptor protein selectively expressed by T cells and natural killer cells." in: The Journal of experimental medicine, Vol. 187, Issue 7, pp. 1157-61, 1998 (PubMed).

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