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Linker For Activation of T Cells (LAT) antibody

Antigen

Linker For Activation of T Cells (LAT)

Synonyms LAT1, pp36, MGC108666, LAT, MGC157208, lat
Clonality Monoclonal
Host
Alternatives

Rabbit

Reactivity
Alternatives

Human

Conjugate
Alternatives Un-conjugated
Application
Alternatives Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunoprecipitation (IP)
2 references available
Certificates ISO 9001:2008
Catalog no. ABIN649976
Quantity 100ul
Price 450.00 $   Plus shipping costs $45.00
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Additional Information

Alternative name LAT
Gene ID 27040
UniProt O43561
Characteristics Rabbit Monoclonal Antibody
Specificity A synthetic peptide corresponding to residues surrounding GRB2-binding motif 2 of human LAT was used as immunogen. This antibody detects both long and short forms of LAT.
Molecular Weight 36/38 kDA
Comments

Background: LAT (linker for activation of T-cells) is involved in the T-cell antigen receptor (TCR) signal transduction pathway, and may play an important role downstream in activating protein tyrosine kinases (1). LAT is phosphorylated by ZAP-70/Syk protein tyrosine kinases leading to recruitment of multiple signaling molecules (2). It has been found that Tyr191 and Tyr171 are required for T-cell activation and Tyr132 is required for the activation of PLCgamma1 and Ras signaling pathways, respectively. Tyr226 and Tyr191 are required for Vav binding, whereas Tyr171 and Tyr132 are necessary for association and activation of phosphoinositide 3-kinase activity and PLCgamma1 (3).

Application Details

Application Notes The suggested dilution is: WB: ~~ 1:500 IP: ~~ 1:60 FC: ~~ 1:40 IHC: ~~ 1:100
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Storage LAT Antibody can be stored at -20°C for up to 12 months from time of receipt.
Research Area Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Structure
Restrictions For Research Use only

Publications

Product Zhang, Sloan-Lancaster, Kitchen et al.: "LAT: the ZAP-70 tyrosine kinase substrate that links T cell receptor to cellular activation." in: Cell, Vol. 92, Issue 1, pp. 83-92, 1998 (PubMed).

Weber, Orstavik, Torgersen et al.: "Molecular cloning of the cDNA encoding pp36, a tyrosine-phosphorylated adaptor protein selectively expressed by T cells and natural killer cells." in: The Journal of experimental medicine, Vol. 187, Issue 7, pp. 1157-61, 1998 (PubMed).

Alternatives

Alternatives for antigen "Linker For Activation of T Cells (LAT)", type "Antibodies"
Hosts Rabbit (101), Mouse (37), Goat (4), Sheep (1)
Reactivities Human (136), Rat (Rattus) (79), Mouse (Murine) (76), Chicken (19), Cow (Bovine) (19), Dog (Canine) (19), Rabbit (18), Cat (Feline) (1)
Applications Western Blotting (WB) (84), Immunofluorescence (IF) (47), ELISA (45), Immunoprecipitation (IP) (33), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (20), Flow Cytometry (FACS) (16), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (9), Immunohistochemistry (IHC) (8), Immunocytochemistry (ICC) (7), Immunoelectron Microscopy (IEM) (3), Immunohistochemistry (Frozen Sections) (IHC (fro)) (2), Dot Blot (DB) (1)
Conjugates Alexa Fluor 488 (5), PE (5), Alexa Fluor 350 (3), Alexa Fluor 555 (3), Alexa Fluor 647 (3), Biotin (3), Cy3 (3), Cy5 (3), Cy5.5 (3), Cy7 (3), FITC (3), Gold (3), HRP (3), PE-Cy3 (3), PE-Cy5 (3), PE-Cy5.5 (3), PE-Cy7 (3), APC (2), Alexa Fluor 700 (2), Dyomics 647 (2)
Epitopes C-Term (30), pTyr200 (20), pTyr161 (19), Cytoplasmic Domain (11), Internal Region (4), pTyr191 (4), AA 31-233 (3), pTyr220,C-Term (3), pTyr171 (2), AA 30-113 (1), AA 70-120 (1), C-Term,AA 191-221 (1), N-Term (1), pTyr132 (1), pTyr226 (1)